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Which enzyme will they use to stick the fragment and the plasmid together?
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- MVHLTPEEKSAVTALWGKVNVDEVGGEALGRLLVVYPWTQRFFESFGDLSTPDAVMGNPKVKAHGKKVLGAFSDGLAHLDNLKGTFATLSELHCDKLHVDPENFRLLGNVLVCVLAHHFGKEFTPPVQAAYQKVVAGVANALAHKYH MVHLTPEEKSAVTALWGKVNVDEVGGEALGRLLVVYPWTQRFFESFGDLSTPDAVMGNPKVKAHGKKVLGAFSDGLAHLDNLKGTFATLSELHCDKLHVDPENFRLLGNVLVCVLAHHFGKEFTPPVQAAYQKVVAGVANALAHKYH MVH ltp eeksavta lwgkvnvdevggealg rllv vypwtqrffesfg dlstp davmgnpkvkahgkkvlgafsdglahl dnlkgtf atlselhcdkhvdpenfrllgnvlcvlahhfgkef tppvqaayqkvvagvanalahkyh MVH ltp eeksavta lwgkvnvdevggealg rllv vypwtqrffesfg dlstp davmgnpkvkahgkkvlgafsdglahl dnlkgtf atlselhcdkhvdpenfrllgnvlcvlahhfgkef tppvqaayqkvvagvanalahkyh…T с A G T TCT TCC TCA TCG CCT ccc CTA CCA CTG CCG ATT ACT ATC Ile ACC Thr TTC TTA TTG CTT CTC Phe Leu Leu ATA ATG Net GTT GTC GTA GTG с Val Ser Pro ACA ACG GCT GCC GCA GCG TAT TAC TAA TAG CAT CAC CAA CAG AAT AAC AAA AAG A Ala Tyr ✰✰✰ 2) Mutant E makes Met-His-Val-Arg His 1) Mutant D makes Met-His-Val-Gly-Asn-Thr G Cys TGA TGG Trp CGT CGC TGT TGC Gln GAT Asp GAC GAA Glu GAG Standard genetic code Asn 5'-ATGICACIGTAIGGT|AAC|ACTIGAGICCC|TGACC-3' 3'-TAC|CTG|CATICCG|TTG|TGA|CTC|GGG|ACTGG-5' Met His Val Gly Asn Thr Glu Pro STOP Lys CGA CGG AGT AGC AGA AGG GGT GGC GGA GGG Arg FURG TUAO HUKO HLAG Ser The DNA segment below encodes the short peptide of 8 amino acids. Mutant strains produce the altered proteins shown below. Arg Using the genetic code table provided, a) describe the exact single base pair alteration that must have occurred in the DNA to give rise to each mutant protein, b) name the kind of mutation it is, and c) show how it leads to the mutant protein. Assume all mutations in each…Second Letter U A G | Phe UCU UUC UUA Tyr UGU UGC Cys /u Stop UGA Stop | A Stop UGG Trp G UUU UAU UAC UCC Ser | UCA UAA Leu UUG UCG UAG CU Leu ccc CAU CAC CAA CUU His CGU CUC CGC | Arg | C Pro CUA CCA Gln CGA A CUG CCG CAG CG AGU Ser U AUU AUC ACU ACC AAU Asn Thr AAC AAA lle AGC AUA АCА AGA | Lys A Arg G AUG Met | ACG AAG AGG GUU GUC GCU GAU GAC Asp GGU GGC GGA U Val |GCC GCA Gly C A Ala GUA GAA Glu GUG GCG GAG GGG G
- 1 10 20 30 40 50 60 70 -I---- 5' АTCGGTCТCGGCTACTACАТАAАСGCGCGCATATATCGAТАТСТАGСТАGСТАТCGGTCTAGGCTACTАC 3' TAGCCAGAGCCGATGATGTATTTGCGCGCGTATATAGCTATAGATCGATCGATAGCCAGATCCGATGATG I--------I--- --I--- --I------ --I--- -I---------I Promoter 80 90 100 110 120 130 140 -I---- 5' CAGGTATсGGTCTGATCTAССТAGCTTCTтсттстстстстсссссGCGGGGGCTGTACTATСATGCGTCG 3' GTCCATAGCCAGACTAGATCGATCGAAGAGAAGAGAGAGAGGGGGCGCCCCCGACATGATAGTACGCAGC -I- -I------- -I--- -I---------I RBS 150 160 170 180 190 200 210 -----I--- ---I-- ------I- ---I---------I---- -I---------I 5' тстCGGCTАСТАCGTAAACGCGCGCATATAтCGATATCTAGCТAGСТАТСGGTстCGGCTACTAсGTAAA 3' AGAGCCGATGATGCATTTGCGCGCGTATATAGCTATAGATCGATCGATAGCCAGAGCCGATGATGCATTT 220 230 240 250 --I----- ---I-- ------I--- -I 5' CCCTATTAGCATGGGTCATATTTGTGTCTGCTTGTTGGGT 3' GGGATAATCGTACCCAGTAGAAACACAGACGAAGAACCCA a. What are the nucleotides of the MRNA from gene Z? b. What are the amino acids encoded by gene Z? ( Vate VSecond letter U A G UUU Phe UUC, U UUA UCU) UCC UCA UAU UAC FTyr UGU] UGCCYS UUG FLeu UCG, Ser UAA Stop UGA Stop A UAG Stop UGG Trp G CAU 1 CGU CGC Arg CUU CCU His CÁC S САА CỤC ССС Leu Pro CỦA ССА CGA CUG CCG CAG GIn CGG AAU LAsn AGU ], AUU ACU* AUC }ile A AUA АСC АСА ААС AAA Ser AGC. AGA Thr JArg AUG Met ACG AAG FLys AGG GAU ASP GACS GAA GAGJ Giu GGG) GUU GUC - Val GUA GCU] GCC GGU GGC Ala Gly GCA GGA Glu GUG GCG Given the double-stranded DNA molecule shown below, what is the sequence of the mRNA corresponding to the coding strand (the one that would be made by RNA polymerase reading the template strand). Label the 5' and 3' termini. Coding strand 5'- ТАTGAAАTTTAAATTT -3' Template strand 3'- АТАСТТТАААТТТАAA — 5' а. What are the amino acid sequences encoded peptides by the three possible reading frames? Please write your answer like this: Pro-His-Stop-Leu etc. Reading frame 1 starts with the first 5' nucleotide. ORF1: Enter your answer here ORF2: Enter your answer here ORF3: Enter…Rh-hr Kell Duffy Xg Results D C E с Kp Kp Js Js Fy Fy Xg AHG 0.2 M DTT + + + 0 + + 2+ 2+ + 0 0 0 0 + 0 + 0 0 0 0 + Neg Neg 0 0 + 0 + + 0 + 0 0 + +2+ Neg +2+ 2+ + + + + 0 + 0 + 0 0 + + 0 + + 0 + 0 + 0 + 0 +2+ 24 + 0 0 0 0 + 0 + 0 0 + + 0 Neg Neg ID4 0 + + 0 0 0 + 0 + 0 + +2+ 2+ Neg Neg ID5 0 0 + 0 0 + 0 + + 0 + 0 + + ID6 0 0 + 0 + + 0 0 0 0 + + + 0 + 0 2+ Neg ID7 0 0 + 0 0 + 0 0 0 + 0 0 0 0 + 0 0 + + Neg Neg ID8 + 0 + + 0 0 + 0 + 0 0 + 0 0 + 0 + + 0 + + Neg Neg ID9 0 0 + 0 0 0 + 0 0 + + 0 0 0 + 0 + + + 0 Neg Neg ID10 000 + 0 0 + + + 0 0 0 + 0 + + 0 + 0 + 0 Neg Neg ID11 000 + 00+ 0+ 0 0 + 0 + 0 + 0 + 0 0 + +Neg Neg AC Neg AC, auto control; SC, screening cells; ID, identification panel; Neg. Negative reaction Note: SC1 to SC3 are screening cells, ID1 to ID11 are panel cells. All screening and panel cells have negative results with the IS phase and Thermo Phase. 1. What is the result of the Antibody Screening test? 2. Which of the following panel cells contain only the Js" antigen? 3.…
- 5’- TTGACATGCG ATGTCGTAGG GATATATAAT CCACACAGTC GACAATTGAC ATCCCGGTCA CCTATAATCG TGAACACATA TAAGGAGTCT GACTATGGTA AGTAAATATG GTCATTAGTT ATGTGAACAG CCATTGGAGT CCACACTGAC TGAGATTAAG AGATGCCGAT AAAGTGGATA GCATTTTAAC TTGACAATGG ACGATCGATC GTAATTACCA ATGTGAATCG TAGTTGCGCA TTTCGGACGT -3’ a) Counting from the first nucleotide (A) of the Shine-Dalgarno consensus sequence, show the sequence of the first 20 nucleotides in the mRNA encoded by this piece of DNA (specify 5’ & 3’ ends). b) What is the sequence of protein X (specify the amino and carboxyl termini)? c)A mutation occurs in the original DNA strand for protein X such that the adenosine nucleotide at position +35 is deleted. What is the effect of this mutation? d) A mutation occurs such in the original DNA for protein X strand such that the adenosine nucleotide at position +48 is changed to a thymine. What is the effect of this mutation?S Savwas Rea lize NGSS ommunity/classes/da298ebd9 c514500827f97e044ab6ee8/assignments/2c3a 98df8d654edaab97cc6399 5c23fd/content/1ccf1054-b803-361d-b16a-8b4 G saint mother teresa. A Sa int Report K Maddox Bry ant - gr. Vs-nhm Growth lan's father keeps bees, and lan spends time observing their behavior. He is especially interested in bee communication and has even seen the waggle dance. The waggle dance communicates the location of food to other bees in the hive. All honeybees know the waggle dance from birth. Choose the correct words to complete the sentences. The waggle dance is an example of a(n) Choose... behavior, because bees can perform it correctly the first time. Bees exhibit Choose... Choose... ovide food and protection for the hive's young. learned predatory courtship instinctiveHindII --- 5' GTC - GAC 3', HaeIII --- 5' CC - GG 3', EcoRI --- 5' G - AATTC 3' and BamI --- 5' CCTAG - G 3' 5' AGAATTCTTACGCCGGACGTACCTAGGTTTAGTCGACTC CGCCGCCCCTAGGGTCATCA 3' 3' TCTTAAGAATGCGGCCTGCATGGATCCAAATCAGCTGAGGCGGCGGGGATCCCAGTAGT 5' Number of pieces of DNA , and blunt end fragment (s), and sticky end fragment(s)
- Refer to the codon diagram on. Which of the following is a codon that will terminate translation? * Phe AGU Cys (C) G Trp (W) G UN A C C Arg (R) Leu (L) A Ser (S) Lys (K) A UG Asn (N) His (H) Thr Gin (Q) (T) Arg (R) lle (1) Ctart 是3 MetOMY Friend are waiting The mall me with for-unit£L -on-for, at - 2 Zeena ndos the bug colleje every day on - at -to - by Head way 3- I Pachaseda cup board my- new hog Plug at- un til-PoY-toIs it correct?