They have purified a 1100 bp HindIII restriction fragment that they plan to sequence. As a first step, they decided to construct a restriction map of the fragment for the enzymes EcoRI and SmaI. Below is shown an agarose gel of the appropriate digests. Draw a restriction map of the fragment and show the distances, in base pairs, between the HindIII, EcoRI, and SmaI sites. *

Essentials of Pharmacology for Health Professions
7th Edition
ISBN:9781305441620
Author:WOODROW
Publisher:WOODROW
Chapter5: Abbreviations And Systems Of Measurement
Section: Chapter Questions
Problem 5CRQ
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They have purified a 1100 bp HindIII restriction fragment that they plan to sequence. As a first step, they decided to construct a restriction map of the fragment for the enzymes EcoRI and SmaI. Below is shown an agarose gel of the appropriate digests. Draw a restriction map of the fragment and show the distances, in base pairs, between the HindIII, EcoRI, and SmaI sites. *

Fragment
+ECORI
Fragment
+Smal
Fragment
+EcoRI + Smal
Standards
Fragment
1,300
1,000
800
a
550
250
100
||
Transcribed Image Text:Fragment +ECORI Fragment +Smal Fragment +EcoRI + Smal Standards Fragment 1,300 1,000 800 a 550 250 100 ||
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