When you make a gene to put into another organism, you need to combine the __________ sequence at the beginning of one gene with the _____________ sequence at the end of another gene.
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- When you make a gene to put into another organism, you need to combine the __________ sequence at the beginning of one gene with the _____________ sequence at the end of another gene.
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- In three sentences describe how Sanger sequencing worksYou want to insert a sequence in the lacI gene to alter its function,what restriction enzyme(s) will you use?Indicate 1 or 2restriction enzyme(s) that you will use on the gene to show the cutWhat are the advantages of using larger restriction fragments when constructing a genomic library of human DNA? Please answer asap and type your answer and do not copy from anywhere please
- Why is it helpful to use a computer program to translatea genetic sequence rather than doing it by hand?You are asked to sequence a piece of DNA to determine if it is from a gene thought to be involved in the development of breast cancer. The sequence of the template strand is ATGCCCGTAATCGTTA and you are given the primer TAACGA. You take these along with a sequencing kit that contains everything else needed for sequencing. You then run the sequencing experiment and analyze the results on a sequencing gel. Which of the following gels (A-D) is the correct sequencing gel for this experiment? Answers: A-D A A BB CC DD Question #3 attachment A ATOC B с A TO C ATOC ATOCUsing the GeneEx Computer Simulation http://intro.bio.umb.edu/MOOC/jsGX/JsGenex_C2.html Complete the following Question and upload a screenshot to this assignment with your unique gene shown. (Use the Snipping Tool to capture a screenshot- it is in the start button and search for snipping tool) Design an entirely new gene that you have invented. Using the new Gene Explorer, this gene should (you can make it more challenging if you like): Produce a protein of at least five amino acids (including the N-terminal Met). Contain at least one intron. Tips 1.Use the “Enter New DNA Sequence” button and delete the starting sequence from the entry blank. 2.Type in a promoter, a little DNA, and a terminator; be sure your RNA is made. 3.Click on your gene and add the start codon, coding region, and stop codon; be sure your protein is made. Type slowly so that the program can keep up. 4. Similarly, add an intron in the coding region and be sure your gene works
- Create a script about genetic manipulation on animals and plants. All contents of the video must be original. Thank you, it's for my project.Separate the word into their word parts and then define each word part. MutagenFrom the results of your BLAST search you can link to the GENE entry for one of your top hits. This link is located under the “Related Information” heading at the right hand side of each displayed alignment (i.e. scroll down to the “Alignments” section). QUESTION What is the “Official Symbol” and “Official Full Name” for this gene?
- You and your team attempt to isolate the gene coding for the defective enzyme. The gene sequence is shown in the table below; the number at the end of the row is the numerical position of the last base in that row, so the 60th position has a “G”. You compare the sequence of the gene from your patient with three other patients you have had with the same symptoms; the mutations for each patient in the table below. What is the amino acid change in the enzyme from your patient (you’ll need to refer to the genetic code)? What amino acid changes are associated with the enzymes from the other three patients? Explain how each of these amino acid changes would lead to non-functional enzymes. Patient Base pair location Mutation Justin 319 C ---> A Patient A 374 G ---> A Patient B 1004 G ---> C Patient C 1144 C ---> TYou have studied about the "Bubble Baby", a condition that is practiced to keep the infection prone baby safe. What is the reason of being infected prone? Write about the available treatments for the disease and the overall details about gene therapy.Aside from Sanger Sequencing, what are the other DNA sequencing methods that are available today? Search for all of them and TABULATE your results. The TABLE should compare Sanger sequencing with the other sequencing methods.