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In what ways is the membrane filter method for coliform detection superior to the most probable number method
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- Another method of microbial control is filtration. List down at least 5 kinds of filters which could be used for this method?Can you please discuss the principles of using Motility Test Medium for the detection of bacterial motility. Focus on the components of the medium and the purpose of each component. Note: You may refer to different culture media manuals like Difco, BBL, etc.What are the possible problems/limitations to be encountered in a laboratory experiment about the Isolation of Foodborne Pathogens on Selective, Differential, and Enriched media (e.g., blood agar, mcconkey, mannitol, XLT-4) using steak plating method. Provide a brief but concise explanation for every limitations, respectively.
- Describe three problems associated with using the standard plate count method for determining the number of bacteria in a sample.If you did the multiple-tube technique, list one advantage and one disadvantage of each method of detecting coliforms?You are transferring a bacterial culture from a tube of broth to a Petri dish containing trypticase soy agar. Please list the steps that are necessary to make the transfer successfully and with minimal risk. of contamination.
- Why are aseptic techniques implemented during inoculation?discuss the clinical significance, principles, reagents (if any) and positive and negative results of the following laboratory tests for Gram positive cocci organisms: Bile esculin test 5% salt turbidity or tolerance test PYRase test Pyruvate broth testGiven the scenario, compute for the total volume of the culture media solution (milliliter or liter) and dehydrated media (grams). Scenario: The students of a Microbiology class were tasked to transfer or subculture a pure culture of Escherichia coli bacterium in five 7 mL nutrient broth and five petri dishes of nutrient agar with 20 mL capacity each. Based on the instruction bottles for nutrient broth and nutrient agar, preparation of the culture media is as follows. Nutrient broth: 8 g/liter Nutrient agar: 28 g/liter Formula: C1V1 = C2V2 *Concentration *Volume Computation: What are the answers to the following. Weight in grams of nutrient broth: _________ Distilled water in mL for nutrient broth: __________ Weight in grams of nutrient agar __________ Distilled water in mL for nutrient agar: ____________
- Why is aseptic urine collection important when cultures are ordered? If you counted 20 colonies from a 0.01-ml inoculum of a 1:10 dilution of urine, how many organisms per milliliter of specimen would you report? Is this number significant? What can you learn from visual inspection of a urine specimen? How would you relate these to the microorganisms present in the sample? How are UTIs acquired/transmitted? Explain why E. coli is frequently implicated in cystitis in females.Below is a scenario requiring the use of laboratory equipment and instruments. A watery fecal sample is tested for the presence of Bacillus cereus and Vibrio cholerae. The sample is stored in a screw capped tube. You are tasked to grow the Bacillus in a solid medium while Vibrio in a liquid medium. Choices: A. Incubator, B Hanging Drop Slide , C.Petri Dish, D. Drying Oven, E. Bunsen Burner, F. Inoculating NeedlE , G. Volumetric Pipette, H. Screw-capped Tubes, I. Hot plate, J. Centrifuge, K. Inoculating Loop, L. Microscope, M. Beaker, N. Biosafety Cabinet 1. What could be used to determine if there are more bacteria in the sample aside from Bacillus and Vibrio? 2. What should you use if you want to observe the natural motility of Bacillus under a Brightfield microscope? 3. Where should you perform the task of sterilization and inoculation? 4.What should be used to transfer the sample to the broth? 5. What could be used to separate the solid from the liquid portion of the…The methodology for enumerating coliforms using the most probable number method calls for presumptive and confirmatory steps. Discuss the reasons why one cannot go directly to inoculation of brilliant green lactose bile broth after homogenizing the sample. 2. Explain why a green metallic sheen is formed when E. coli is grown on Eosin Methylene Blue Agar