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discuss why a mixed culture cannot be used to inoculate a differential media such as the tripple sugar iron agar test.
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- Can you please discuss the principles of using Motility Test Medium for the detection of bacterial motility. Focus on the components of the medium and the purpose of each component. Note: You may refer to different culture media manuals like Difco, BBL, etc.When using Endo agar as the selective and differential media for the confirmed test, how would you know whether the test is positive for the presence of coli?Kligler’s iron agar and SIM are multiple test media.. Both media are stabbed but for different reasons. Explain.
- Kligler’s iron agar and SIM are multiple test media.. What component of both media allows you to detect the preceding characteristic?Given the scenario, compute for the total volume of the culture media solution (milliliter or liter) and dehydrated media (grams). Scenario: The students of a Microbiology class were tasked to transfer or subculture a pure culture of Escherichia coli bacterium in five 7 mL nutrient broth and five petri dishes of nutrient agar with 20 mL capacity each. Based on the instruction bottles for nutrient broth and nutrient agar, preparation of the culture media is as follows. Nutrient broth: 8 g/liter Nutrient agar: 28 g/liter Formula: C1V1 = C2V2 *Concentration *Volume Computation: What are the answers to the following. Weight in grams of nutrient broth: _________ Distilled water in mL for nutrient broth: __________ Weight in grams of nutrient agar __________ Distilled water in mL for nutrient agar: ____________If an organism that showed no growth in the MIC broth test at a particular dose, then grows in an MBC test when plated to media, the concentration of the drug was bacteriostatic. O 1) True O 2) False
- What are the possible reasons why culture plates may have too many colonies despite performing serial dilutions and OD600 readings?Why go to the trouble of creating a master plate (why not simply plate the initial culture on both nutrient agar and glucose-salts agar)?An unknown bacterium produces colorless colonies when inoculated onto an EMB plate. Predict what you would see if you inoculated that same unknown onto the Mannitol salt agar media?
- The students of a Microbiology class were tasked to transfer or subculture a pure culture of Escherichia coli bacterium in five 7 mL nutrient broth and five petri dishes of nutrient agar with 20 mL capacity each. Based on the instruction bottles for nutrient broth and nutrient agar, preparation of the culture media is as follows. Nutrient broth: 8 g/liter Nutrient agar: 28 g/liter Answer the following: a. What is the weight in grams of nutrient broth? b. What is the weight in grams of nutrient agar? c. What is the distilled water in mL for nutrient broth? d. What is the distilled water in mL for nutrient agar?The statement, “In the laboratory, a sterile inoculating loop is moved across the agar surface in a culture dish, thinning a sample and isolating individuals", describes which technique?discuss the significance of the different aseptic techniques utilized in maintaining sterility of the cell culture lab.