In this experiment, the problem is that the volume of the volumetric pipet is not accurately known (only 2 sig figs), so any density calculation will also be limited to 2 sig figs. Calibration increases the number of sig figs from 2 to 4. As a result, the density also increases from 2 to 4 sig figs. Explain how and why this is possible.
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- In this experiment, the problem is that the volume of the volumetric pipet is not accurately known (only 2 sig figs), so any density calculation will also be limited to 2 sig figs.
- Calibration increases the number of sig figs from 2 to 4. As a result, the density also increases from 2 to 4 sig figs. Explain how and why this is possible.
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- The diagram shows a simplified output from the detector with peaks for two compounds X and Y. The areas under the peaks are shown in green. What, if anything , can you say about the relative concentrations of X and Y in the mixture? Explain your answer.Normal calibration curves are generally run in water (pure solvent). If there is something in your unknown sample "matrix" which affects the analytical signal, you'll report an incorrect concentration. In this situation you'll choose to run a "standard addition" calibration curve. Standard addition entails adding the same amount of unknown to each of your standard solutions prior to analysis. This transforms your independent variable from "concentration" to "added concentration". These leads to an entirely different calculation for your unknown concentration. A standard addition procedure can be used with any analytical method. In this problem we're conducting a fluorescence assay for a blood protein which is a cancer marker. For the following problem plot a standard addition curve from the data shown below, and calculate the concentration for your unknown. Your unknown number is: 770 Solution 1 is your unknown diluted 25:1 and has an intensity of 5.94 Solution 2 is your unknown…Column Chromatography of 9-Fluorenone Experiment Introduction: Column chromatography is an important chromatography method used to separate and purify organic compounds. Column chromatography is typically used to separate impurities and purify products from chemical reactions, including separating unused starting material from products. Column chromatography is very similar to Thin Layer Chromatography (TLC) because it relies on a solid stationary phase (absorbent) and liquid mobile phase (eluant) to separate organic compounds based on their different polarities. In column chromatography, the stationary phase is a solid absorbent, typically silica gel or alumina, with a liquid coating packed into a glass column. The stationary phase is typically very polar. An eluant (mobile phase) solvent travels down the column and the separation occurs as the organic compounds enter into equilibria with the stationary and mobile phases. The polarity of the eluant may be adjusted from non-polar…
- An orange compound, dissolved in dichloromethane, is added to a chromatogra- phy column. The elution is begun using hexane as the solvent. After 6 L of solvent was passed through the column, the orange band had still not traveled down the column appreciably. What should be done to make this experiment work better.Is it possible for a single molecule to test true positive in all the qualitative assays? Why or why not?This is the graph for gel filtration chromatography. Can you please explain why the graph has the maximum and minimum peak and relate it with the gel filtration concept such as the volume of elution, molecular weight, and so on. Thank you . Graph for Result 1: (Volume of elution (mL) with 1% bromophenol blue) Volume of Elution (mL) vs Corrected OD 1.789 1.8 1.6 1.4 1.2 0.745 0.8 0.477 0.6 0.379.400.452 0.406 0.393 0.254 d28 0.25 0.4 9:128 0.161 0.115 0.2 0.060 2 6. 10 12 14 16 Volume of Elution (mL) Corrected OD
- What does the %RSD, listed in the spectral data, as well as the correlation coefficient of your standard curve, tell you about the quality of the data and integrity of the results? my standard % RSD is 36.4%, I had to calculate manually from the 5 standard solutions I did have on the spectra. The R squared value from the standard curve is 0.7282You find that for your analysis, your response factor (adjusted for internal standard) for a particular analyte was 0.0104 ppm. When you run a sample mixture, the integration for that component is 2,186, and the integration for the internal standard is 14,139. What is the concentration of the component in ppm? (Enter the numerical value without units.)In this experiment, it is assumed that No. of moles of (Ca2+ + Mg2+) = moles (Ca2+). Calculate the number of moles of Ca2+ in the hard water sample. please show working out, thank you Sample No. 1 2 3 4 Final Burette reading (mL) 24.40 46.90 24.70 47.20 Initial Burette reading (mL) 0.10 24.40 2.10 24.70 Final-Initial Titre (mL) 24.30 22.50 22.60 22.50
- Which error will lead to negative deviation from the theoretical Keq? a. cuvette was filled to the brim with the sample. b. cuvette was washed with sodium hydroxide instead of distilled water. C. standard with the highest concentration was used in autozero. d. cuvette was misplaced such that the opaque side faces the lightA gas chromatogram of a mixture of toluene and ethyl (a) Measure w1/2 for each peak to the nearest 0.1 mm. When the thickness of the pen trace is significant relative to the length being measured, it is important to take the pen width into account. It is best to measure from the edge of one trace to the corresponding edge of the other trace, as shown at the bottom of the left column. (b) Find the number of theoretical plates and the plate height for each peak.Introductlon In Part (a) of this experiment you will prepare a primary standard. Potassium hydrogen phthalate (KHP) is a monoprotic acid with the formula KHC,H.O. In this experiment KHP is used as the primary standard. A standard solution is one whose solute concentration is accurately known. If a solute can be obtained in a very pure, stable, weighable form, a primary standard solution of it can be prepared directly. You will then use the standardized sodium hydroxide solution to determine the acetic acid content of a dilute unknown solution. Procedure (0) Preparation of a Primory Standard Potassium hydrogen phthalate (KHP) is a primary standard. Weigh accurately by difference, approximately 0.8 g of KHP in a clean, dry weighing bottle and transfer it into a 250 ml conical flask. Add 75 ml of distilled water. Swirl gently. Cover the flask and leave to dissolve, swirling periodicaly. Cautlon: KHP is not highly soluble and care must be taken to ensure it is all dissolved before you…