This is the graph for gel filtration chromatography. Can you please explain why the graph has the maximum and minimum peak and relate it with the gel filtration concept such as the volume of elution, molecular weight, and so on. Thank you .

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Chapter4: Least-squares And Calibration Methods
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This is the graph for gel filtration
chromatography. Can you please explain why
the graph has the maximum and minimum
peak and relate it with the gel filtration
concept such as the volume of elution,
molecular weight, and so on. Thank you .
Graph for Result 1: (Volume of elution (mL) with 1% bromophenol blue)
Volume of Elution (mL) vs Corrected OD
1.789
1.8
1.6
1.4
1.2
0.745
0.8
0.477
0.6
0.379.400.452
0.406
0.393
0.254
d28 0.25
0.4
9:128
0.161
0.115
0.2
0.060
2
6.
10
12
14
16
Volume of Elution (mL)
Corrected OD
Transcribed Image Text:This is the graph for gel filtration chromatography. Can you please explain why the graph has the maximum and minimum peak and relate it with the gel filtration concept such as the volume of elution, molecular weight, and so on. Thank you . Graph for Result 1: (Volume of elution (mL) with 1% bromophenol blue) Volume of Elution (mL) vs Corrected OD 1.789 1.8 1.6 1.4 1.2 0.745 0.8 0.477 0.6 0.379.400.452 0.406 0.393 0.254 d28 0.25 0.4 9:128 0.161 0.115 0.2 0.060 2 6. 10 12 14 16 Volume of Elution (mL) Corrected OD
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