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- A surgical technologist working in the Central Sterile Processing Department is asked to run the steam sterilizer (autoclave) with a biological monitor for the first load of the day without any instrument trays or items. Which type of bacteria would be used to test the autoclave?Loop with bacterial inoculum was subjected to flame sterilization prior to streaking on agar plate. Effect: Rationale:Technique used to inoculate plate media using inoculating loop. Technique used to inoculate agar deep using inoculating needle. Pattern used to inoculate agar slant to get luxuriant culture. This pattern increases the surface area of agar that can be inoculated. Can you answer all the questions? Thankyou!
- Oven sterilization of aqueous culture medium. Effect: Rationale:Mechanism of moist-heat sterilization is by Select one: DNA damage Oxidative processes require higher temperature than Dry heat Denaturation and Hydorlytic damageCan culture media be sterilized in the hot air sterilizer? Why or why not? (Answer in not more than 5 sentences)
- List 5 other methods of sterilization and describe the principle/s and when each method is used. What are the requirements to support microbial growth?What are the primary uses of the following preparations of culture media? Agar regular slant Agar special slant Agar deep Broth Agar plateOf what practical importance are air borne microorganisms to the laboratory workers? What precautions should be taken to control laboratory contaminants? Why are petri dishes incubated in an inverted position? Of what advantage is the using a solid and a liquid medium?
- Of what practical importance are air borne microorganisms to the laboratory workers? What precautions should be taken to control laboratory contaminants? Of what advantage is the using a solid and a liquid medium?Identify the method of sterilization/ chemical agent described At 15psi, the temperature reaches 121 degree Celsius requiring only 15 to 20 minutes to sterilize the material. The material to be sterilized is exposed to live steam for 30 minutes for 3 consecutive days.Lid of petri dish was left on bench top for a few minutes after pouring of sterile medium. Effect: Rationale: