How do photopigments separate when performing paper chromatography? Most soluble pigment will stay lower on the paper Heavier pigment will stay lower on the paper Most soluble pigment will stay higher on the paper Heavier pigment will stay higher on the paper
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- What chemicals are used for the Simple Stain from our lab experience? Check all that apply. carbolic acid mycolic acid phenol acid fuschin malachite green safranin nigrosin iodine ethanol methylene blue crystal violet carbol fuschin Congo red acid-alcohol O O O O O O O O O O O O O ODuring studying a thick heavily stained specimen under the microscope, it is better to light intensity adjustment has nothing to do with the clarity of the specimen. increase the light intensity passing through the specimen decrease light intensity passing through the specimen none of the options is correctThese cells are being viewed under high power. Use the length of just one cell to estimate the number of cells that can fit into the FOV. Scanning power objective = 5X; Low power objective = 40X; High power objective = 100X; Eyepiece = 10X; Low power field of view (FOV) = 1.5 mm
- Is acid fast staining differential stain , if yes, what are the 2 types of stain usedDuring staining, the first step is to dewax the slide. What is the purpose of the step? What is the effect of insufficient dewaxing?Which one of these techniques does not distinguish between live and dead cells? Group of answer choices Coulter count fluorescence staining spread plate
- For each stain, indicate if it’s a cationic dye or an anionic dye (2 pts). Stain Cationic or Anionic Dye malachite green crystal violet nigrosin safraninIodine in Ziehl Neelson’s staining serve as the mordant. True or false underline the word that is falseWhat is the primary stain for the Ziehl-Neelson acid fast stain? What color is this stain? What cells will display this stain once you finish the stain and view your slide? Paragraph BIU
- Which spectrophotometer mode should you use to calibrate the instrument and which mode should you use to read your samples? Transmittance for both Absorbance for both Absorbance to calibrate and transmittance to read samples Transmittance to calibrate and absorbance to read samples Flip a coin to pick a mode each time.A student using a microscope measured the low power field diameter to be 7.1mm. The student then switched from the low power (4x) objective lens to the medium-power (10x) objective lens. Assuming a 10 x ocular (eyepiece) lens, the medium power field diameter is ____ um um is micrometersIf a specimen is being viewed under a 40x objective, and the microscope has a 10x ocular lens, what is the total magnification? 1x 4x 10x 100x 400x