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During staining, the first step is to dewax the slide. What is the purpose of the step? What is the effect of insufficient dewaxing?
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- What are the most commonly used methods in staining? why?What are the pros and cons of using the TCE stain-free method versus a traditional gel staining method (CBB staining)?Describe how simple staining and fluorescence staining are similar and how they are different. What are the advantages of each?
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- What is the primary stain for the Ziehl-Neelson acid fast stain? What color is this stain? What cells will display this stain once you finish the stain and view your slide? Paragraph BIUThis part of the staining process helps the primary stain to remain in the cell during decolorization. O 1) Secondary stain O 2) primary stain O 3) counterstain O 4) fixing reagent O 5) mordantWhich step is the most crucial or most likely to cause poor results in the Gram stain?