Jessica placed crystal violet ,grama's iodine and safranin reagents on her bench.Which staining procedure is she preparing for with these reagents?

Q: Explain the reasons you would use a negative stain?

A: In a negative stain the slide is actually stained. This is because the negatively charged stain…

Q: Is it possible to perform double or triple staining using fluorescent dyes? Explain

A: For both membrane and soluble proteins, fluorescent dyes are increasingly being utilized to monitor…

Q: What kind of stain would a pathologist use to visualise a chrondrosarcoma under the microscope? why…

A: Staining is a method for enhancing contrast in material, usually on a tiny scale. Biological…

Q: What is the usual stain used for staining a sputum smear?

A: Essentially, sputum comprises of a combination of saliva and mucus. additionally named as phlegm, It…

Q: Let's assume that you were teaching the Gram stain to four students, and each student did one thing…

A:

Q: is it essential that the primary stain and counterstain be of contrasting colors

A: Gram staining is one of the crucial type of differential straining .It divides bacteria into two…

Q: What are the sources of error in performing Gram staining?

A: Bacteria can be divided into two categories namely gram positive(with thin layer of peptidoglycan)…

Q: What is the principle application of negative staining?

A: Microbiology is the branch of biology that deals with study of organisms that are too small to be…

Q: What are the underlying physical principles of paper chromatography? What is spectrophotometry, the…

A: Analysis of different compounds quantitatively and qualitatively is an important aspect of research…

Q: Transillumination is used clinically for the detection of:

A: Transillumination is an examination that is done with the help of a strong light shining on a…

Q: What are Romanowsky stains? Name four of these.

A: Staining is a technique used to enhance contrast in samples , generally at the microscopic level.…

Q: What are the pros and cons of using the TCE stain-free method versus a traditional gel staining…

A: PAGE (polyacrylamide gel electrophoresis) is a well-known method for separating, detecting, and…

Q: In gram staining, could colors other than red be used as a counterstain?

A: A counterstain is a stain with color contrasting to the principle stain, making the stained…

Q: In the Ziehl-Neelsen acid-fast stain, what is the mordant?

A: bacteria like  Mycobacterium and Nocardia and the apicomplexan Cryptosporidium are not stained…

Q: Why is there a need to use a staining agent in observing the specimen?

A: Staining agent:          The things that owes-out stains were recognized as staining agent,…

Q: What is the purpose of acid-fast staining? What information can you gain from this procedure? Please…

A: The acid-fast stain is used to identify acid-fast organisms. Acid-fast organisms posess nearly…

Q: In four steps, describe the simple staining procedure.

A: A stain is used to clearly view a specimen under the microscope. In simple staining, bacteria are…

Q: What is a differential stain? why is it used?

A: Differential stain is a type of stain use to identify organisms.

Q: When it comes to simple stains, why is time so important? And what is the purpose of rinsing the…

A: Microbiology is a hugely important field in both basic and applied research. New and fascinating…

Q: What are the most commonly used methods in staining? why?

A: Introduction  Staining is a technique for enhancing contrast in material, usually on a tiny scale.…

Q: Choose the dyes or reagents used in the Endospore stain. (More than one choices are correct)   a…

A: Simple stains- A stain that employs a single stain to color a bacteria: they are quick and easy.…

Q: If you conducted an acid-fast stain on a sputum sample from a person with tuberculosis, what would…

A: The acid-fast staining technique is a differential staining technique. The bacteria which get…

Q: Create paragraphs to differentiate among the following terms: positive stain, negative stain, simple…

A: An organic molecule with a benzene ring, a chromophore, and an auxochrome group is known as a…

Q: Types of Differential Staining techniques.  1. Gram stain 2. Acid-fast stain     Please…

A: Staining is used in microscopy to highlight the structures to be viewed under microscope. It is of…

Q: The following figures are the photomicrographs of the samples taken with Kern&Sohn Camera Microscope…

A: Ans: Lugols solution: The Lugols solution is 2.5 to 25% weight by volume total iodine.

Q: What are the principle and basic concepts of Simple staining

A: The simple staining doesn't give a lot of data about the cell separated from the bacteria'…

Q: Which step of the gram stain procedure is most critical?why?

A: Gram staining is one of the most common laboratories test to identify the different bacterial…

Q: Give three situations where the negative staining procedure would be used.

A: Negative staining is a specimen preparation technique that requires an acidic dye such as India Ink…

Q: name 5 things about method staining

A: INTRODUCTION Staining Staining is a method that is used in medical field and histology to see things…

Q: Why is it necessary to use contrasting stains while staining

A: Introduction:- Cell staining is a technique that allows you to see cells and cell components more…

Q: What is the secondary stain in both acid-fast stain methods?In the Ziehl-Neelsen acid-fast stain,…

A: Staining is a technique used in slide preparation to color the specimen. With light microscopy,…

Q: Why is it called as differential stain

A: Staining is a technique used in slide preparation to color the specimen.With light microscopy,…

Q: Positive Staining Negative staining

A: Staining Procedure Dye Charge Of The Chromophore/ Colored Ion Charge Of The Target Cellular…

Q: What major advantage does phase-contrast microscopy haveover staining?

A: Phase-contrast microscopy is an optical microscopy technique used in cells. When the light passes…

Q: What is a crystal violet stain and its purpose?

A: Crystal violet is that the name given to the cluster of similar organic compounds that are used as…

Q: Describe the bacteria observed in different staining procedures. Please refer to the photo - Direct…

A: The cell wall of most bacteria has an overall net negative charge and thus can be stained directly…

Q: Write the instructions, reagents, principles and the procedures for the following.  1. Periodic acid…

A: (AS PER OUR GUIDELINES WE ARE PROVIDING YOU ANSWER OF 1ST TWO QUESTIONS. YOU CAN POST OTHER…

Q: What is the primary stain for the Ziehl-Neelson acid fast stain? What color is this stain? What…

A: In microbiology, the technique that is used to enhance the contrast in samples, at the microscopic…

Q: Which of the following is/are true regarding the acid-fast stain? (There may be more than one…

A: Answer  First option  Non-acid-fast microbes appear blue in a completed acid-fast stain.

Q: Draw what you would expect to see for acid fast stain and endospore stain procedure if you did not…

A: only acid fast appears red/pink. Only endospore appears green. Vegetative cell become colourless.

Q: Why is it incorrect to say that the GFP is a “staining” method?

A: Green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that…

Q: What is the differences between ocular magnification and objective magnification?

A: Introduction  A microscope's objective lens is the one near the sample at the bottom. It's an…

Q: Why is it necessary to cut thin sections of the tissue sample from a specimen using a microtome?

A: Microtomes are specialized cutting instruments that produce very thin slices of tissues so that the…

Q: What chemicals are used for the Simple Stain from our lab experience? Check all that apply. carbolic…

A: Simple staining: * The process staining the bacterial cell with a dye  to see bacterial structure…

Q: do we need to stain microorganisms?

A: The study of microorganisms is termed microbiology. Microorganisms is microorganism, fungi, archaea…

Q: Does the gram staining method still have a place in today's world of advanced diagnostic methods? If…

A: Gram staining is a type of staining which is used to differentiate bacteria on the basis of…

Q: Name two fixatives used in staining?

A: Histology is the study of ultrastructure of tissues using thin sectioning techniques like microtomy,…

Q: Specific Dyes used in flagella stain: A wet-mount procedure (Ryu method) Dried-smear preparation…

A: Flagella is a hair-like structure that is found in bacteria, archaea, and Eukaryota. It allows the…