Have a look at the results in this SDS-PAGE gel. A MW ladder is shown. In the "control" lane, a sample of recombinant protein "LBT3" was loaded. Lane A shows proteins that have been prepared from leukocytes from a normal, healthy individual. Lanes B, C, D, E and F show protein prepared from leukocytes from individuals with lymphoma. What is the best explanation for the result shown for patient D? 200 150 100 75 50 37 20 Weight, kDa MW ladder Control A
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- In (b), why would it be more efficient to use labeled anti-human IgG rather than label the patient’s antibodies?Give typed explanation a patient presents with leukocytosis comprised mostly of large blasts with moderate basophilic cytoplasm. some blasts have vacuoles. molecular analysis reveals the cMYC gene rearrangement. what immunologic markers would you expect on these blasts?An injection contains 5 million international units (MIU) of interferon alpha-n3 (ALFERON N) proteins per milliliter. The recommended dose is 0.05 mL. The literature states that the activity of interferon alpha-n3 is approximately equal to 2.6 × 108international units/mg of protein. Calculate (a) the number of international units and (b) the micrograms of interferon alfa-n3 proteins administered per dose.
- What is the meaning of (Helf-life) for immunoglobulin ?such as the helf life for IgG is 23 day.!!???What is the result of the Antibody Screening Test? Based on the result of the autocontrol, it is safe to say that the antibodies which reacted with the screening cells and panel cells are?Give 5 storage conditions of human normal immunoglobulin injection and why?
- You are interested in performing indirect immunofluorescence light microscopy to observe the localization of the catalase enzyme in the cultured HeLa cells, obtained historically from the cervical tumor of Henrietta Lacks. You were going through the lab stock and found a few primary and secondary antibodies. Which of the following secondary antibody can you use in your experiments? O All of the mentioned antibodies can be used in the experiment Goat anti-human antibody conjugated to 10 nm gold Goat-anti-human catalase conjugated to 10 nm gold O Human anti-catalase antibody conjugated to fluorescent rhodamine Goat anti-human antibody conjugated to fluorescent rhodamine2. https://doi.org/10.1186/s12868-022-00692-1 (link to research) a) In the immunohistochemistry section of the materials and methods section the authors wrote “The number of positive cells in hotspot areas in ten high power fields (HPFs) in areas of demyelination and plaques in the brain stem were counted using the image analysis software (Lecia Application Suite Version 4.12.0, Welzlar, Germany).” Why were they looking at demyelination areas for this study? b) In the effect of mitoxantrone on histopathological changes in the brain section of the results section the authors wrote “Active plaques revealed inflammatory cellular infiltrates with abundant macrophages stuffed with myelin debris, an evidence of ongoing myelin breakdown.” What does macrophages stuffed with myelin debris have to do with the study?Shortly after the recovery from COVID-19, the plasma of the convalescent has significant quantities of anti-SARS-CoV-2 antibodies. This plasma was suggested to be used as therapy for other COVID-19 patients by transfusing the plasma from the recovered individuals (donor) to the other COVID-19 patients (recipient). Do you think the identification of the ABO group of the donor and recipient is important and why? O a. No, this has nothing to do with blood grouping. It is only related to the virus and the antibodies against it. O b. Yes, but I really have no idea why. O c. No, not important because the antigens A, B or AB exist on the surface of red blood cells but not in the plasma and when they transfer, they do not transfer cells (only plasma) O d. Yes, because the donor plasma might contain anti-A or Anti-B or both which might induce an immune response if the recipient has A or B or AB group. Clear my choice
- 1) SDS PAGE gels can be probed with NBT/BCIP to perform an immunoblot true/ false 2) the direction of migration of protiens through a matrix is due to most proteins in natur having a net negative charge true/false 3) PBST is a buffer that is used for blocking the membrane during immunoblotting. true/false 4) the buffer used during for transferring proteins to nitrocellulose methanol. true/false 7) The size of the pores of a polyacrylamide gel dcreases as concentrations of polyacrylamide increases. True or false 10) The secondary antibody used in laboratory for western blotting is conjugated to which enzyme A) Alkaline decarboxylase B) GAPDH C) Alkaline Phosphatase D) Horseeradish peroxide 13) In protien electrophroresis, what reagent present in the sample buffer is used to eliminate difference in the charge densities of protein A) SDS B) 2- mercaptoethanol C) Tris-HCL pH6.8 D) Tris- HCL PH 8.8c) State some of the features that cross the cell membrane and make "porin proteins" specific. d) Briefly clarify the concepts of "acylation", "prenylation" and "GPI stabilizer" in the context of membrane protein interactions. e) When determining ABO Blood Groups, give information about which blood group data can be obtained depending on the interaction of which antigens in the red blood cell and which antibodies in the serum.Why is type AB considered a universal acceptor of blood from donors? A. Type AB erythrocyte membrane contains the basic tetrasaccharide structure of the H-antigen. B. Type AB erythrocyte membranes contain pentasaccharides that has the basic tetrasaccharide unit linked to either galactose or N- acetylgalactosamine C. Type AB erythrocyte membrane contains the same Rh saccharide units together with the pentasaccharide antigenic determinant characteristic of both A and B type. D. Both A and B E. All of these