Calculate the volume of TAE buffer that you will need to prepare a 100ml solution of 1X strength if you only have a 50X concentrated solution available.
Q: Calculate the energy(Kcal.) produced from full oxidation of (3) mole of glucose (glucose --> CO2+…
A: Complete oxidation of 3 moles of glucose gives 657 (or 700.8) kcal energy. This difference is due to…
Q: There are various types of DNA-targeting drug, including DNA alkylating agents, DNA intercalators…
A: Cancer means uncontrolled cell division. There are several therapeutic agents are used to treat…
Q: Criven below givers are some statement in which of the Coenzymes withe their vitamin enyame and…
A: Coenzymes are essential for some Enzymes to carryout their catalytic activity. Coenzymes can alone…
Q: Which of the following regarding disulfide bonds is/are true?
A: Proteins are polymers of amino acids which are linked by peptide/amide bonds and form primary…
Q: Q1. Diffusion is: Directional Non-random Passive None of the above
A: The body's biochemical and physiological processes involve a variety of molecules and solutes.…
Q: Which of the following is true regarding microbial tests that lead to color change in tubes used to…
A: Option 1 and 4 are correct. There are multiple tests involving color change for bacteria…
Q: 10. Certain molecules in the muscular system must be maintained in a homeostatic range for the…
A: Muscular system maintains homeostasis by movement, support and heat production. There are certain…
Q: True or false Ketone body synthesis requires transportation of acetyl-CoA through the inner…
A: Acetone, acetoacetate and 3 beta hydroxybutyrate are known as ketone bodies. They are produced from…
Q: the correct amount that can be resolved from the TLC?
A: There are apperenttly 8 Isomers of ketohexoses which are D-Fructose, D-tagatose, D-psicose, and…
Q: Administration of insulin decreases the blood concentration of ketone bodies in patients with…
A: The cells inside the body of people who are diabetic are unable to uptake glucose from blood.…
Q: 18. For DNA sequencing using the Sanger Method and radioactivity, four separate reactions (A, G, C…
A: Introduction: The DNA sequencing method was developed by Frederick Sanger and his colleagues in the…
Q: The transition state means that: a. fewer molecules have the energy required to reach the…
A: Enzymes are protein molecules that increase the rate of reaction by decreasing the activation…
Q: Hi! Can you give an explanation for each sample identity in paragraph form (for the rationale)?…
A: Introduction: Carbohydrates are large macromolecules that contain three essential elements which are…
Q: II. ILLUSTRATIONS. For each of the given proteins: Draw the final location of the following proteins…
A: Proteins produced in the cytosol contain a Nuclear Localisation Signals (NLS) whose presence in the…
Q: write true if the statement if correct and change the " " word/phrase to make it correct…
A: Chromatography is biochemical separation method for organic molecules or solutes of a compound…
Q: When an inappropriate amino acid is substituted in place of another, as occurs in certain genetic…
A: Though proteins are made up of hundreds of amino acids but each amino acid is important in the…
Q: Consider the function of the cofactor FAD. Which of the following makes it unique (different) from…
A: Nicotine adenine dinucleotide NAD+ and flavin adenine dinucleotide (FAD+) are coenzymes that play…
Q: Calculate the energy(Kcal.) produced from full oxidation of (3) mole of glucose (glucose --> CO2+…
A: The removal of electrons is called oxidation, and the gain of electrons is called reduction. In the…
Q: heating the starch-amylase solution after the allowed time interval
A: An enzyme that breaks down starch is known as amylase.
Q: What is bio chemistry
A: The word biochemistry is the combination of two words biology and chemistry. In simple terms…
Q: 7.0, each into e 2 in ne ding 1. Du kan 8 10 6 itions. TABLE 2 Effect of pH on the Hydrolysis of…
A: Hydrolysis of starch is carried out in the presence of strach digesting Enzyme called amylase.…
Q: write true if the statement if correct and change the bold word/phrase to make it correct lower…
A: Glycogen is the storage form of carbohydrates in animals. It is also called animal starch for this…
Q: A dehydration reaction will create (large or smaller) molecules by removing a molecule of (water or…
A: A molecule is a collection of atoms linked together by various types of bonds. A macromolecule is…
Q: What is the role of Glycosyl transferase
A: Enzymes: are the biocatalyst that increases the rate of reaction without going change in itself. It…
Q: General formula of alkyne compounds are.
A: Alkynes are organic molecules specially unsaturated hydrocarbons . this molecule contain one or more…
Q: THCA 100 This reaction is Entropy is THC ( Leafly Mur AD RNA polymerase ATGACGOATCAGCCOCAAG…
A: Tetrahydrocannabinol Acid when heated converts into delta-9-tetrahydrocannabinol. the reaction is…
Q: Given the active site diagram below, identify the mechanism(s) of catalysis. 2 1 5 Metal ion Induced…
A: Acid-base catalysis, metal ion catalysis, approximation etc are some of the mechanisms through…
Q: The top side of this figure offers more opportunities (for each base pair) that can lead to highly…
A: A single stranded nucleic acid is formed by joining the nucleotide units together through…
Q: pMDawn is digested with EcoR1, and BamHI. Resulting in fragments shown below: EcoRI: 20 kb BamHI:…
A: Given: Digestion of a plasmid PMDawn with EcoRI, BamHI, and EcoRI+BamHI are as follows: EcoRI: 20 kb…
Q: Which among the following catalyse dehydration step in the TCA cycle ○ Isocitrate dehydrogenase O…
A: The acetyl CoA molecules synthesized through oxidative decarboxylation of pyruvate enter into the…
Q: Match the specificity pocket (identified by red number) to the residue that occupies it. 1 3 4 2 1.…
A: Proteases preferentially hydrolyze polypeptide substrate peptide bonds based on the amino acids…
Q: the maximum amount of ATP that could be generated by the full oxidation of the compound…
A: In the given question, the fatty acid mentioned is CH3(CH2)4COOH. Here full oxidation means…
Q: draw the hydrogen bonds for the following nucleic acid base pair: G and C
A: In nucleotides, nitrogenous bases are aromatic heterocyclic substances. Purines and pyrimidines are…
Q: Choose all minor nucleosides Anenosin-cyclomonophosphate Thymidin Pseudouridin Pyromycin
A: Nucleosides are the compounds made up of nitrogen base and sugar residues. Minor Nucleosides are…
Q: Polymerase with H2N-...GMMTVPPKKKRVGMMTV...-COOH Provide the amino acid sequence of the signal…
A: The amino acid sequence of the signal peptide is-…
Q: Beta-carotene is a prescursor for: OA. Vitamin A O B. Riboflavin O C. Folate O D. Vitamin D
A: “Since you have asked multiple questions, we will solve the first question for you. If you want any…
Q: A 22- carbon fatty acid enters the B-oxidation metabolism and then proceeds to the knebs cycle.…
A: An example of a fatty acid with 22 carbon atoms is Behenic acid. The beta-oxidation is the…
Q: What are the advantages and disadvantages of using the Ehlrich test for proteins Suggest how the…
A: Introduction: Ehlrich test is a biochemical test that is performed to detect a particular amino…
Q: The Krebs cycle is considered to be an anabolic pathway because some of the reactions are
A: Krebs cycle: Krebs cycle also know as TCA cycle or citric acid cycle is an important part of aerobic…
Q: Which of the following are not driving forces for protein folding i. Salt Bridges ii. Conformational…
A: Proteins have four levels of the structural organization including Primary, secondary, tertiary,…
Q: Which of the following units recognize UAG, UAA, and UGA codons? (Single answer) Elongation factors…
A: Genetic code is the combination of 3 nucleotide (called nucleotide triplet or codon) where each…
Q: The Hershey-Chase experiment, in which bacteria were infected with radioactively labeled bacterial…
A: The Hershey-Chase experiment, that also involved infecting bacteria with radioactively labelled…
Q: Given the active site below, which best describes the mechanism(s) of catalysis? 5 NH 2 *H₂N. HN O…
A: Active site: The area of an enzyme's active site is where substrate molecules interact and engage…
Q: @write a detail note on citric acid Discuss mechalis menten equation. cyde.
A: Metabolic pathways that occur inside the cell are used to break down the large molecules into…
Q: 9. Cholesterol is not readily broken down by cells and does not serve as a source of fuel. It can…
A: Cholesterol is a lipid with amphipathic properties. Along with phospholipids, they can be located on…
Q: Given the active site below, which best describes the mechanism(s) of catalysis?
A: Active site of the enzyme is the region present on the enzyme where enzymatic catalysis occurs and…
Q: What is the total number of hydrogen bonds that exist between the DNA strand 5’-TTCAGAG-3’ and its…
A: Adenine and guanine are the purine bases which occur in the nucleic acids. And thymine, cytosine,…
Q: Calculate the standard free-energy change, deltaG'o, for the reaction in which acetaldehyde is…
A: Relevant reaction and their standard reduction potential: Acetaldehyde + 2H+ + 2e- →ethanol…
Q: The key to biopolymer self-replication is: O Natural selection O Intramolecular complimentarity O…
A: Biomolecules are organic compounds produced by living organisms. They play important function as…
Q: Which of the following is likely to be correct if all mutases are completely inhibited? a. Removal…
A: Mutases are the Enzymes that catalyse the transfer of functional groups from one position to other…
Calculate the volume of TAE buffer that you will need to prepare a 100ml solution of 1X strength
if you only have a 50X concentrated solution available.
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
- If 4 mL of 1 M NaOH is added to 100 mL buffer, would it still be a usable buffer according to the conventions? Explain why or why not.Calculate pH of buffer prepared by mixing; 10ml of 0.10M of CH3COOH and 20ml of 0.10M of CH3COONa.To make 200 ml 0.4XSSC (Saline and Sodium Citrate) solution for FISH post-washing, how many ml 20X SSC solution is needed?
- How do you prepare 500 mL pH 6 Citric acid/Phosphate buffer in the laboratory. Please explain briefly.3 mL of a 45 mM stock solution of a substrate is added to 8 mL of water. Calculate the following values. The substrate molecular weight is 125 g/mol. Calculate the following values: Substrate Volume in mL Dilution Factor Substrate number of moles Substrate concentration for the diluted solution in mM Substrate concentration for the diluted solution in mole/L Substrate concentration for the diluted solution in mg/mLHow much of the enzyme proteinase k (solute) is required to make 250ml of a solution with a concentration of .01mg/ml? (Weight/volume ratio)
- Make a list of the five most critical qualities of an effective extraction buffer. Please provide a brief description of each.What would be the working stock concentration (in molarity) if you diluted 250 uL of a stock concentration of 10 uM in a total volume of 1 mL?How much of a 10x buffer should be added to 900ml of water to make a 1x solution?
- Calculate the appropriate volume (in µL) of 9X loading buffer that should be added to 46.0 µL of a sample prior to loading the sample on an agarose gel.can you show how to calculate the amount of two components for the required volume of a buffer with the receipes? Prepare enough 10X stock solution § Prepare 1LProportions§ 0.25M tris§ 1.92M glycine§ pH 8.3§ 1% (w/v) SDSInstructions to prepare 100mL of 10X running buffer1. Weigh 3.02g of Tris base and 14.42g of glycine into ~90mL of ddH2O in a clean beaker and stir todissolve.2. Check the pH with a calibrated pH metre once dissolved.§ The pH of the buffer should be 8.3. pH adjustment is not required if between 8.1-8.5.3. Add 5mL 20% (w/v) SDS stock solution (a liquid) to make a 1% (w/v) solution.4. Top the volume to 100mL by adding 5mL ddH2O from a graduated cylinder.If 400 mL of a 20% w/v solution were diluted to 2 L, what would be the finalpercentage strength?