pMDawn is digested with EcoR1, and BamHI. Resulting in fragments shown below: EcoRI: 20 kb BamHI: 11 kb, 6 kb, 3 kb EcoRI + BamHI: 8 kb, 6 kb, 3 kb Which would be the most appropriate ladder for the gel that you would pour?

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
icon
Related questions
Question

pMDawn is digested with EcoR1, and BamHI. Resulting in fragments shown below:

EcoRI: 20 kb

BamHI: 11 kb, 6 kb, 3 kb

EcoRI + BamHI: 8 kb, 6 kb, 3 kb

Which would be the most appropriate ladder for the gel that you would pour?

A B C D
DNA Mass
(ng/5μl)
25
67
30
27
30
Base Pairs
||||||||||||||
1500
1200
1000
800
700
600
500
450
400
350
300
250
-200
-150
100
-50
2% TAE agarose gel
900
1% agarose
bp
4,000
3,000
2,000
1,650
1,000
850
650
500
400
300
200
100
XCFF-
Tartrazine-|
bp
-2072
-1500
-900
-600
-300
-100
DNA Mass
(ng/5μl)
16
28
28
28
28
18
92
34
34
20
92
23
30
45
Base Pairs
| | || |
25K
10K
8K
6K
5K
- 4K
- 3K
-2.5K
<<-2K
-1.5K
- 1K
-750
- 500
- 250
0.7% TAE agarose gel
Transcribed Image Text:A B C D DNA Mass (ng/5μl) 25 67 30 27 30 Base Pairs |||||||||||||| 1500 1200 1000 800 700 600 500 450 400 350 300 250 -200 -150 100 -50 2% TAE agarose gel 900 1% agarose bp 4,000 3,000 2,000 1,650 1,000 850 650 500 400 300 200 100 XCFF- Tartrazine-| bp -2072 -1500 -900 -600 -300 -100 DNA Mass (ng/5μl) 16 28 28 28 28 18 92 34 34 20 92 23 30 45 Base Pairs | | || | 25K 10K 8K 6K 5K - 4K - 3K -2.5K <<-2K -1.5K - 1K -750 - 500 - 250 0.7% TAE agarose gel
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 2 steps

Blurred answer
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON