hat is an ONPG test? WHat is he principle behind this assay.
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What is an ONPG test? WHat is he principle behind this assay.
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- why are lysozyme and ovoalbumin the recommended standards used in the Bradford protein assay and why would they be preferred over BSA being this widely used but not recommended? Justify the answerWhat does MEGA stand for and what is this assay used to determine? How to do perform the assay?What is the purpose of Southern's blotting technique? Explain in detail the biochemical principle that underpin each step of the method.
- Why is the bradford assay method using BSA sensitive ? and how does it compare to the amino acid analysis methodAmong various dye-based assays like Biuret, Lowry, Bicinchronic Acid, and Biuret Protein Assays, what can be done if a concentrated sample is out of the detection range of chosen dye-based assay?Describe the p-nitrophenol assay and how a standard curve can be used to calculate the concentration of an unknown .