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Why is the DNS method used in glucose assay?
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- Why is the bradford assay method using BSA sensitive ? and how does it compare to the amino acid analysis methodWhat is an ONPG test? WHat is he principle behind this assay.Why is it essential to add sodium azide to succinate dehydrogenase assay solution? What is the purpose of including the sodium succinate solution?
- What does MEGA stand for and what is this assay used to determine? How to do perform the assay?why are lysozyme and ovoalbumin the recommended standards used in the Bradford protein assay and why would they be preferred over BSA being this widely used but not recommended? Justify the answerWhat is the difference between in-ovo and ex-ovo types of CAM Assay?
- Why are the correct primer sequences essential for successful amplification?What is the purpose of including a tube in the phage assay in which only the E. coli culture is inoculated? Explain.What reaction would you expect when performing a positive control in the catalase assay? What would it mean if a known catalase-positive bacteria did not produce the expected reaction?