Esc F2 F5 F6 FB F9 F10 F11 5. What is the physical appearance (solid, liquid, semi-solid) of the following media: broth tube, agar slant tube, agar deep tube, and agar plate. What are you testing when you introduce an inoculum with a needle on an agar deep tube?
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A nutrient media is a type of media that is used for the growth of organisms at laboratory conditions. It contains all the nutrient requirements for an organism. There are different types of media in the laboratory:
- Basal media
- Enriched media
- Selective media
- Indicator media
- Transport media
- Storage media
- Broth media
- Agar media etc.
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- Prepare the following strength solution: Prepare 250 mL of 1/2 strength tube feeding. The nurse would add mL of water to mL of tube feeding to get 250 mL of 1/2 strength tube feeding solution.What physical appearance (Solid, liquid, semi-solid) of following Media: Broth tube, agar slant tube, agar deep tube, and agar plate. What exactly am I testing when I Introduce an inoculum with a needle on an agar deep tube?How many grams each of 1% and 5% hydrocortisone ointments are required to prepare 30 grams of a 15% ointment? Answers A -F 7g of 1% ointment and 1 g of 5% ointment 3.75 g of 1% ointment and 26.25 g of 5% ointment C 30 g of 1% ointment and 4.29 g of 5% ointment D 7.5 g of 1% ointment and 22.5 g of 5% ointment E 22.5 g of 1% ointment and 7.5 g of 5% ointment
- Answer the following questions (not more than 5 sentences/question). Discuss the correct laboratory technique in using the centrifuge for qualitative analysis. Give two tips on its proper use. Explain how calcium ions are confirmed present in a solid salt by using flame test. Discuss a method to confirm the presence of a) phenol and b) ketone in a test compound. How are proteins confirmed present in a sample? Explain the laboratory process.Identify whether the statements are TRUE or FALSE 1. You should use P-20 micropipettes to transfer volumes for spread plating. 2. You should use P-200 micropipettes to transfer volumes for pour plating.Answer the following questions based on Kirby Bauer / MacFarland standards 1-0.5 McFarland standard solution is ideally used for inoculation of plated for Kirby Bauer test. a)True b)False Principle of Kirby Bauer test is the diffusion of antibiotic agents in to Agar based media. a) True b) False
- A 1.5-year-old child developed vomiting, diarrhea, and fever. Stool sample were inoculated into the Endo media. After 18 hours on the surface of the medium grew medium-sized, round, slightly convex red colonies with a metallic luster. The doctor suspected Escherichia coli. 1. Name the composition of the Endo agar media. 2. Describe the properties of bacterial colonies on the Endo media. 3. What purpose differential diagnostic Endo media used for?With a plate count average of 297 and a final dilution on the plate of 1:1,000 ; what is the viable number of cells in original culture (CFU/ml)?How would you prepare a 1:500 dilution? You cannot use more than 10mL per tube.
- Prepare 50 plates of Sheep's Blood Agar using 100mm petri dish. Add 10% of blood to the media. (Manufacturer's instructions: Suspend 38 g of blood agar base dehydrated powder in 1 liter of distilled or deionized water.) Pour 25 mL of prepared medium per plate. Express answer to the nearest whole number. How many grams of the dehydrated powder should be weighed? Answer: What is the volume of distilled water that should be used in the preparation? Answer: What is the volume of sheep's blood that should be added to the sterilized and cooled blood agar base? Answer:Which of the following is the a-anomer? H но H Но H2C H ОН H2C- нн ОН со 4 1 2 1 Submit 3 -OH Н ОН -OH ОН Н there is no a-anomer ОН ОН Н Request Answer H Но НО Н H2C нн ОН 2 H2C ОН -OH 4 Н ОН н н H OH -О. ОН ОН Н ОН НList the reasons for using blood agar.