ect in ePortfolio idol™M → Caffeine Acetaminophen Solvent front Download 14. A student conducted an experiment using the silica gel TLC plate shown below by spotting a sample of MidolTM, caffeine, and acetaminophen on the plate and eluting with petroleum ether: chloroform (9:1, v/v). Separation of Amino Acids by Thin Layer Chromatography Q Print A Alternative formats A B
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Separation of Amino Acids by Thin Layer Chromatography Lab Questions. Thank you for your help!
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- Chitinase is a protein that breaks down chitin, a primary component of the cell wall in fungi, scales in fish and exoskeletons of arthropods. The activity of chitinase extracted from a plant was shown to be optimum at pH 5. You were tasked to prepare 300 mL of 150 mM buffer solution for further analysis of the extracted chitinase. REAGENTS Ka 2.5M Acetic acid Solid NaOAc•3H2O [136.08g/mol] 1.76 x 10-5 2.5M NH3 Solid NH4Cl [53.49g/mol] 5.6 x 10-10 2.5M Lactic acid Solid sodium lactate [112.06g/mol] 4.0 x 10-5 5 M HCl 5M NaOH Pls show sol'ns 1. Given the following reagents, give the moles of each component (acid & base).2. What are the mass/volume of the components needed to prepare the buffer? 3. What will the pH of the buffer be if 1mL of 5 M NaOH was added?Penicillin G potassium 5000 units Isotonic Sodium Chloride Solution ad 15 mL Sig. Nose drop Using soluble penicillin tablets, each containing 100,000 units of crystalline penicillin G potassium, explain how you would obtain penicillin G potassium needed in compounding the prescription.Computation: Ratio Strength, PPM, mg%Show your complete solution.1. How many grams of a 1:80 trituration of strychnine sulfate should be used for this prescription?RxStrychnine sulfate 45mgBelladonna extract 360mgSucrose 36gFt. chart; Div. in #XXXVIAmount of Strychnine in grams = ?
- Rx Clindamycin phosphate 4% w/v Propylene glycol 12.9% w/v Purified water 11% w/v Isopropyl alcohol ad 244 mL (Sp.G. = 0.880) Ft. clindamycin gel Sig.: Place thin layer onto affected areas How many grams of isopropyl alcohol are present in a clindamycin gel that has 76 mL of purified water? Assume negligible volume shrinkage as result of mixing these ingredients. Answer: 415.2 (please show all work) ANSWER IS ALREADY GIVEN PLEASE SHOW WORK IN DETAIL HOW IT COMESDQuestion 21 GC and HPLC were used in this course as: O synthetic techniques analytic techniques purification techniques O liquid-liquid extraction techniques D Question 22 Based on the boiling point of acetone (56° C), 3-methylbutylacetate (141°C), and 3-methyl-1- butanol (131°C), which compound will be the first to come off (elute) the GC column? O acetone O 3-methylbutylacetate O 3-methyl-1-butanol10 tab of acetaminophen 500 mg molar absorptivity= 14520 @ 243nm. The total weight of the 10tab is 5278 mg. I take a sample equivalent to 100mg of tablet and I diluted it in 100mL, I took an aliquot of 10mL and I diluted it in 250mL, of these I took 25mL and I diluted them in 250mL. Calculate the weight per tablet. Absorbance = 0.944
- Benzalkonium chloride (E=0.16) 1.5g qs 100 ml Distilled water 0.9% Nacl solution ad Make isotonic solution Questions: 1. How many ml of distilled water must be added to make the Benzalkonium chloride preparation isotonic? 2. How many ml of 0.9% NaCl must be added to make a 100ml solution?Example of a Protein Purification Scheme: Purification of the Enzyme Xanthine Dehydrogenase from a Fungus Volume Total Total Specific Percent Fraction (mL) Protein (mg) Activity Activity Recovery 1. Crude extract 2. Salt precipitate 3. Ion-exchange chromatography |4. Molecular-sieve chromatography 5. Immunoaffinity chromatography 3,800 22,800 2,460 0.108 100 165 2,800 1,190 0.425 48 65 100 720 7.2 29 40 14.5 23 1.8 275 152.108 11 Calculate the specific activity of step#4. Note that percent recovery=% Yield.80mL of a 0.3M solution of hexapeptide Leu-His-Cys-Glu-Asn-Arg is adjusted to pH=pI. The solution is then titrated with 0.2M HCL to a final pH of 2.1. Sketch the titration curve, labelling the pH and volume axes. Indicate the volume of HCL needed to reach relevant pKa value and equivalence point(s)z Relevant pKa values are: 2.1, 4.3, 6.0, 8.3, 9.8, and 12.5.
- Calculate the appropriate volume (in µL) of 3X loading buffer that should be added to 24.0 µL of a sample prior to loading the sample on an agarose gel.Why do we compute for TFM in soaps. How does one calculate for TFM? What is saponification? Show its basic chemical equation What are the Natural Moisturizing Factors (NMF) of the skin? Provide their function. 4. Why are carbomers used in a liquid soap/body wash? Name at least two commercial brands of carbomers.Using DEAE-cellulose as ion exhange resin, indicate the starting and ending pH for the narrowest experimental pH range used to seperate an amino acid mixture consisting of Ala, Cys and His Starting pH: Ending pH: