Given the following reagents, give the moles of each component (acid & base). 2.  What are the mass/volume of the components needed to prepare the buffer?

Curren'S Math For Meds: Dosages & Sol
11th Edition
ISBN:9781305143531
Author:CURREN
Publisher:CURREN
Chapter21: Heparin Infusion Calculations
Section: Chapter Questions
Problem 1.3P
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Chitinase is a protein that breaks down chitin, a primary component of the cell wall in fungi, scales in fish and exoskeletons of arthropods. The activity of chitinase extracted from a plant was shown to be optimum at pH 5. You were tasked to prepare 300 mL of 150 mM buffer solution for further analysis of the extracted chitinase.

REAGENTS

Ka

2.5M Acetic acid

Solid NaOAc•3H2O [136.08g/mol]

1.76 x 10-5

2.5M NH3

Solid NH4Cl [53.49g/mol]

5.6 x 10-10

2.5M Lactic acid

Solid sodium lactate [112.06g/mol] 

4.0 x 10-5

5 M HCl

5M NaOH

 

Pls show sol'ns

1. Given the following reagents, give the moles of each component (acid & base).

2.  What are the mass/volume of the components needed to prepare the buffer? 

3. What will the pH of the buffer be if 1mL of 5 M NaOH was added?



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