Compute the volumes needed to complete this table. Reaction Stock Final Volume for Volume for 5x Component Concentration Concentration 1x reaction reaction sddH,0 up to 25 ul up to 25 ul Buffer (ViBuffer A) 10X 1X MgCl, 50 mM 3 mM DNTP 2 mM 100 uM forward primer 10 uM 0.2 uM reverse primer 10 uM 0.2 uM Taq polymerase 1 U/ ul 1 U/ 25 ul DNA template 1-2 ng 1 ul *5X reactions = 2 samples, 1 NTC, 1 positive control, 1 pipetting allowance %3D
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- Match the number in the image with the correct name for the lab supply 1 2 ♡ AGEN QIAGEN QIAGEN QIAC RIVCEM БИЗӘДІС RNeasy Mini Kit (50) Cat. No. 74104 Store at room temperature (15-25°C) WEZAIO QIAGEN 2 GDIVERM QIAGL K (20) QIAGL умени DAIO QIAG GAGEN GIVCEN QIAGEN QIAGEN ИЗОМО QIAGEN NEA QIAGEN QIAGEN N QIAGEN QIAGE Buffer W AGEN Lysis buffer 45 m Buffer RPE Wash buffer 11 ml concentrate to ustan 5 m Butter R U 3 clear collection tube centrifuge tube pink column (with filter) PhaseLock tube pink column (with filter) inside a clear collection tube <Please answer these question. I need these for lab. PRE-LAB ASSIGNMENT – (YOU WILL NOT BE ALLOWED TO BEGIN WORK ON LABWITHOUT THE COMPLETED CALCULATIONS) 1. COMPLETE ALL CALCULATIONS REQUIRED FOR TABLE 1 ( For all measurements, the [PNPP] concentration will be 600 μM in a 1 ml final volume.) PNPP (substrate) stock solution: 100 mM PNPP in buffer 2. IN YOUR LAB NOTEBOOK, DRAW YOUR PREDICTION OF WHAT THEFOLLOWING GRAPHS MIGHT LOOK LIKEa. pH vs. V0b. TEMP vs. V03. OUTLINE THE BEST/MOST EFFICIENT WAY TO PREPAREKCI for injection contains 40 mEq in 20 mL. An order requires 15 mEq, how many mL are needed?
- Your 67 YO 195lb, 5ft 10in male patient diagnosed with testicular cancer has been prescribed ifosfamide 1200mg/M2/day IV for 5 days. You calculate the BSA using the mosteller method as being 2.09m2. Ifosfamide is available in 60mL vials contianing 3g of drug. a) How many mL will you administer each day?A glucose solution is supplied in a repeated fed batch culture. The following parameters have been measured at t=2.5 hours; the system was under a quasi-steady state at this time. V = 1.0L, F = 0.22L/hr, S_0 = 98g/L, mu_m = 0.31/hr, K_s = 0.14 g-glucose/L, YM_X/S = 0.48 g-cells/g-glucose, Xt_0 = 33g (1) Calculate the value of V0 and S. (2) Calculate the value of Xt for t = 2.5hrs.Insulin Lispro subcutaneously for a patient weight 80 kg for 1 month of treatment. Daily dose of insulin is 1 Unit/kg and 60% in short acting, given 4 times per day; ampoules in form of personal injectors (300 Units/3ml). (I would like to figure out the number of personal injector needed for 1 month.) My answer: Daily dose (DD) for a 80 kg patient = 80 x 1 Unit = 80 U 60% is short acting, so DD = 60% of 80 U = (0.6 × 80) U = 48 U Given 4 times per day, so for a single dose = 48 U/4 = 12 U For 1 month (30 days), 30 x 48U = 1440 U Rp. Insulin lispro in ampoules personal injectors 300 Units/3 ml No. ??? (my teacher write is 10 but I don't understand why) Sig. Inject subcutaneously 12 Unit (0.12 ml) before each meal, 4 times a day for 1 month.
- For a gradient system with a gradient of 5-90% in 50 min, flow 2 mL/min, column 100 x 4.6 mm i.d., the first peak elutes at 20 min, and the last peak elutes at 50 min. Calculate k* for this system. 2. Can isocratic elution be used for this sample? If so, what is the range of k values to be expected. 3.Propose a way to shorten the gradient run time by eliminating the wait for the first peak to elute at 20 min. 4. For the original conditions of this question, change the conditions necessary to use a 150 x 4.6 mm column while maintaining the same k* value.Your 64YO 152lb, 5ft 8in female patient daignosed with advanced bladder cancerhas been prescribed ifosamide 1500mg/m2/day IV for 5 days. You calculate the BSA using the Mosteller method as being 1.82m2. Ifosfamide is available in 60mL vials containing 3g of drugs. How many mL will you administer each day?The order is for 1000ml of 5% D/W, 1000ml of Ringers Lactate, 500ml of NS to run over a 36 hour period. The drop factor is 15gtt/ml. How many gtt/min will you administer? using dimensional analysis and show work
- Ibuprofen for infusion comes in vials containing 400 mg of ibuprofen in 4 mL. Scotty requires 410 mg of ibuprofen to be infused every 6 hours. The solution for infusion has a concentration of 2.5 mg per mL. Calculate the volume of 5% glucose solution required to dilute the concentrated solution. Answer: 0.44 mL X *********Calculate the volume of BSA stock that will be required to make the standard solutions needed to create the BSA standard curve. Be sure to show your work and include the volume of 0.02 M phosphate buffer required to reach a final volume of 1 mL. From a 2,000 μg/mL BSA stock, create 1 mL of each of the following stock solutions in 0.02 M phosphate buffer using individual microcentrifuge tubes: 50 μg/mL, 250 μg/mL, 500 μg/mL, 1,000 μg/mL, 1,250 μg/mL, 1,500 μg/mL. Be sure to properly label all the microcentrifuge tubes before creating the standards.AE, 62, Male Wt: 75 kg, Ht: 150 cm Orders: Penicillin G Potassium 10,000,000 Units Sterile water for injection qs Make a 250,000 U/mL solution. The package insert states If Penicillin G Potassium 10,000,000 Units is reconstituted with 44 mL sterile water, a solution of 200,000 U per mL will be obtained. What volume of sterile water would you use to reconstitute the penicillin G potassium in order to make the ordered solution?