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- Draw the products of the reaction of xylulose-5-phosphate and erythrose-4-phosphate catalyzed by transketolase in the pentose phosphate pathway. Provide the structure in the protonation state found in physiological conditions. H H H OH FO HO-H H-OH H OPO3²- Q transketolase Draw glyceraldehyde-3- phosphate H H- H H H O OH OH OPO3²- Draw fructose-6- phosphate Q I IIndicate the general type of enzyme that mediates each glycolysis reaction depicted below. (e.g. Transferase, Oxidoreductase, Kinase, Hydrolase, Lyase, or Isomerase) iii. O H Glyceraldehyde-3-phosphate → 1,3-Bisphosphoglycerate H-C-OH + NAD CH₂O-P + P₁ H-O- -C-H Glucose →→ Glucose-6-phosphate H b 0 OH HO OH H-C- 0 -H HO- H ОН H-C-OH + NADH +H+ CH₂O P P ATP ADP 1 H (P-O- -C- H H HO OH 2-Phosphoglycerate → Phosphoenolpyruvate 0 OH H ОН -O~(P) + HOHWhat product is formed when dihydroxyacetone phosphate is reacted with NADH in the presence of the enzyme glycerol 3-phosphate dehydrogenase? Assume any stereogenic center has the R configuration. This reaction is one step in the synthesis of glycerol (propane-1,2,3-triol), a starting material needed for the biosynthesis of triacylglycerols. НО. dihydroxyacetone phosphate
- In another key reaction in glycolysis, dihydroxyacetone phosphate (DHAP) is isomerized into glyceraldehyde-3-phosphate (GAP): CH,OH | C=0 НС — ОН AG" = +7.5 kJ/mol CH,OPO,?- CH,OPO,- DHAP GAP Because AG° is positive, the equilibrium lies to the left. (a) Calculate the equilibrium constant, and the equilibrium fraction of GAP from the above, at 37 °C. (b) In the cell, depletion of GAP makes the reaction proceed. What will AG be if the concentration of GAP is always kept at 1/100 of the concentration of DHAP?Myristoleic acid is a monounsaturated fatty acid found in small amounts in a variety of foods. Calculate the net ATP yield from the complete β-oxidation of myristoleic acid. The formula of myristoleic acid is shown below (it is assumed that the total ATP production is the same for both saturated and unsaturated fatty acids having the same carbon chain length). CH3-(CH2)3-CHCH-(CH2)7-COOH (Given: The oxidation of one NADH yields 2.5 ATP; the oxidation of one FADH2 yields 1.5 ATP; and the oxidation of one acetyl CoA yields 10 ATP. ) Group of answer choices a. 96 ATP b. 92 ATP c. 94 ATP d. 34 ATP e. 36 ATPHow many ATP molecules can be generated from one mol of 14CH3-COOH? (The conversion of acetate to acetyl-CoA requires the consumption of 2 ATP.)
- The table lists the standard free energies (AG°') of hydrolysis of some phosphorylated compounds. Compound Phosphoenolpyruvate (PEP) 1,3-Bisphosphoglycerate (1,3-BPG) Creatine phosphate ATP (to ADP) Glucose 1-phosphate Pyrophosphate (PP;) Glucose 6-phosphate Glycerol 3-phosphate kJ mol-¹ -61.9 -49.4 -43.1 -30.5 -20.9 -19.3 -13.8 -9.2 kcal mol-1 -14.8 -11.8 -10.3 -7.3 -5.0 -4.6 -3.3 -2.2The degradation of glycogen is catalyzed by the enzyme phosphorylase and has AGO" equal to +3.1 kJ · mol1. The equation for this reaction is shown below. glycogen (n residues) + P;→ glycogen (n-1 residues) + G1P What is the ratio of [P;]/[G1P] under standard conditions? Use 2 significant figures. [P;] : [G1P] = i :1 What is the value of AG under cellular conditions when the [P;/[G1P] ratio is 50/1? Use 2 significant figures. AG = i kJ. mol-1Acetyi CoA Oxaloncetate CoA NADH Citrate NAD Isocitrate Malate Funiarate NAD co NADH FADH, FAD a- Ketoghutarate Succinate co, NAD+ ATP Succinyl CuA NADH ADP - P For each molecule of glucose (C6H12O6) oxidized by cellular respiration, how many moles of CO2 are released in the citric acid cycle? (see the figure above) а. 2 b. 4 с. 6 d. 0 е. 3
- Which reaction is irreversible? oxidized glutathione + NADPH + H+ → reduced glutathione + NADP+ ribulose 5-phosphate → ribose 5-phosphate glucose 6-phosphate + NADP+ → 6-phosphoglucono-δ-lactone + NADPH + H+ sedoheptulose 7-phosphate + glyceraldehyde 6-phosphate → fructose 6-phosphate + erythrose 4-phosphate ribulose 5-phosphate → xylulose 5-phosphateWhich class of enzymes catalyzes the following reactions? CH2- CH2- CH2 H2- C- CH2 AST CH2-C CH2 CH-NH + C=O + C=0 + CH-NH ČO0 Čo0 ČO0 ČO0" Aspartate Oxoglutarate Oxaloacetate Glutamate Select one: a. Oxidoreductase O b. Isomerase O c. Ligase d. TransferaseH H H-C-OH CH-Q HOOHH H OH Glucose H-C=O H-C-H H H-C=0 ATP ADP H-C-OH HO-C-H H-C-OH Н Pyruvate OH Hexokinase ATP ADP IVE pyruvate kinase H-C-O-P-0 Glucose 6-phosphate (PEP) GLYCOLYSIS H-C-O-P=O H-C-OHO-H-C-OHO kinase isomerase но Phosphoenolpyruvate H-C-OH ÇO ATP ADP HO-C-H H-C-OH HO-C-H Phosphofructo H-C-OH aldose H-C-OHỌ H-C-O-P-0 HO Fructose 1, 6-bisphosphate H-C-O-P=O H 6- Fructose 6-phosphate H H-C-O-P=O C=O 6- Fructose bisphosphate H-C-OHO ATP 9 H H-C-O-PO C=O o- HO-C-H Dihydroxyacetone phosphate H ADP Glyceraldehyde 3-phosphate dehydrogenase 2X Triose phosphate isomerase H c=0 H-C-OHO H-C-O-PO 3-phosphate Hỏ NAD + P NADH+H* -O-P=O Ó c=0 Glyceraldehyde N H-C-O-P-O Enolase H-OHO phosphoglycerates-C-O-P-0 phosphoglycerate HC-OHO kinase H H-C-O-P=O Ĥ 6- 2-Phosphoglycerate 3-Phosphoglycerate 1,3-Bisphosphoglycerate