Calculate AGo' for the reaction NADH + pyruvate → NAD+ + lactate You are given: Faradays constant F = 96.5 kJ/mol.V %3D Eo' : NAD+ / NADH = 0.320V Eo': Pyruvate/lactate = -0.185 V А. 13 kJ/mol В. 97 k j/mol С. 49 kJ/mol D. 26 k j/mol Е. None of the above
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- Carbonic anhydrase catalyzes the hydration of CO. CO2 + H2O ¬ H½CO3 The Km of this enzyme for CO, is 1.20×104 µ.M. When [CO,] = 3.60×104 µM, the rate of reaction was 4.50 umol·mL! sec-1 a What is Vmax for this enzyme? umol·mL-!sec-!Given that the reduction potential Eo'=-320, +10, +816, and +50mV for NAD+, fumarate, 02 and G3P DH bound FAD, respectively, calculate the free energy for a pair of electrons originating from the oxidation of glycerol-3-P to DHAP of, as it traverses the ETC. R = 8.315 x 10 J/mol K; Faraday constant, F= 96.48 KJ/V-mol, assume standard state T= 25°C and physiological conditions are T= 37°C. Report a whole number. Remember a Reduction potential is DEFINED as X(ax) +e- --> X(red) Be sure you have defined your oxidation and reduction species properly. kJ/moleThe equation for the overall reaction that occurs in Complex I is shown below: NADH + H* +Q= NAD+ + QH2 End of Chapter_Problem 32a Calculate AG for the reaction. Use 3 significant figures. AG" kJ- mol1 (Tolerance is +/- 2%) eTextbook and Media Save for Later
- a) Calculate the enzyme and specific activity of a reaction with 3 pM Hsp90 using the following information: The rate is measured in a spectrophotometer as 0.028 OD units/min in a 1 ml reaction volume. The absorbance was detected at 340nm and the extinction coefficient for NADH at this wavelength is 6200L M- 1 min-1 and the molecular mass of Hsp90 is 82.7 kDa. The rate of NADH utilisation is equivalent to the rate of ATP utilised by Hsp90. Show all your calculations and the units for your answers. b) Calculate the turnover number for the reaction described in (a) aboveBegining with 1 M concentrations of each reactant and product at pH=7 and 25.0 degrees C, calculate the K'eq of the reaction Pyruvate + NADH Lactate + NADH+H+. Note the temperature of this reaction will not affect the standard reducton potential delta E° in the table 13-7b.What is the Vmax of an enzyme at 1 μM with -4G RT k = 6.2x 10¹2 s-1 (Ideal gas constant R = 8.314 J K-1 mol-1) cat 0.14 μM S-1 1.4 µM s-1 14 μM s-1 0.14 mM s-1 1.4 mM s-1 activation energy (AG) of 55000 J/mol at 298K?
- The standard reduction potential for ubiquione (A or coenzyme Q) is .045 V, and the standard reduciton potential (E) for FAD is -0.219 V. Using these values, show that the oxidation for FADH2 by ubiquinone theoretically liberates enough energy to drive the synthesis of ATP. Faraday constant =96.48KJ/Vol delta G' standard for ATP Synthesis is +30.5 KJ/mol R=8.314 J/mol K=1.987 cal/mol Ka) Calculate the enzyme and specific activity of a reaction with 3 μM Hsp90 using the following information: The rate is measured in a spectrophotometer as 0.028 OD units/min in a 1 ml reaction volume. The absorbance was detected at 340nm and the extinction coefficient for NADH at this wavelength is 6200 L M-1 min-1 and the molecular mass of Hsp90 is 82.7 kDa. The rate of NADH utilisation is equivalent to the rate of ATP utilised by Hsp90. Show all your calculations and the units for your answers. b) Calculate the turnover number for the reaction described in (a) aboveWhat is the Vmax of an enzyme at 1 µM with an activation free energy (AG#) of 55000 J/mol at 298K? -AG # RT k cat = 6.2× 10¹² (Ideal gas constant R = 8.314 J K¯1 mol-¹1) 0.14 μM s-1 -1 S-1 1.4 μM s 14 μM s-1 0.14 mM s-1 1.4 mM s-1
- . For parts (a) and (b) of this problem use the following standard reduction potentials, free energies, and nonequilibrium concentra- tions of reactants and products: АТР - 3.10 mм P; = 5.90 mM ADP = 220 µM glucose = 5.10 mM pyruvate = 62.0 uM NAD+ = 350 µM NADH = 15.0 μΜ CO, = 15.0 torr half reaction E (V) NAD+ + H+ + 2e→ NADH -0.315 2Pyruvate + 6H* + 4e → glucose -0.590 pyruvate + NADH + 2H+ AG" = -64.4 k)/mol → ethanol + NAD+ + CO, ATP + H,0→ ADP + P; + H* AG" - 30.5 kl/mol (a) Consider the last two steps in the alcoholic fermentation of glu- cose by brewer's yeast: pyruvate + NADH + 2H* » ethanol + NAD* + CO, Calculate the nonequilibrium concentration of ethanol in yeast cells, if AG = -38.3 kJ/mol for this reaction at pH=7.4 and 37 °C when the reactants and products are at the concentrations given above. (b) Consider the degradation of glucose to pyruvate by the glycolytic pathway: glucose + 2ADP + 2P; + 2NAD+ →2 pyruvate + 2ATP + 2H,0 + 2NADH + 2H* Calculate AG for this reaction…Begining with 1 M concentrations of each reactant and product at pH=7 and 25.0 degrees C, calculate the K'eq of the reaction Pyruvate + NADH Lactate + NADH+H+. Note the temperature of this reaction will not affect the standard reducton potentialBegining with 1 M concentrations of each reactant and product at pH=7 and 25.0 degrees C, calculate the K'eq of the reaction Pyruvate + NADH <=> Lactate + NADH+H+.Note the temperature of this reaction will not affect the standard reducton potential delta E'o in the table 13-7b.