SHOW COMPLETE SOLUTION AND EXPLAIN. Problem 1Yeast cells has been cultured on glucose (Table 1). The growth data follows the Monod EquationTable 1: Growth data for yeast cellsGlucose concentration (mg/L)710154020010000.12 0.20.3040.340Specific growth rate (h-¹)Observed yield, Y'x/s (g/g)0.066 0.0880.294 0.330 0.364 0.419 0.451 0.458a) Calculate the maximum specific growth rate (μmax) and the substrate constant (Ks)

The Monod equation for cell growth kinetics is given below; μ = μmax [S]Ks + [S] here 'μ' is…

Answered: Problem 1 Yeast cells has been cultured…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Problem 1 Yeast cells has been cultured on glucose (Table 1). The growth data follows the Monod Equation Table 1: Growth data for yeast cells Glucose concentration (mg/L) Specific growth rate (h²) Observed yield, Y'xjs (g/g) 10 15 40 200 1000 0.066 0.088 0.12 0.2 0.304 0.340 0.294 0.330 0.364 0.419 0.451 0.458 d) Calculate the substrate consumption rate (rs) assuming a substrate concentration of 40 mg/L and a biomass concentration of 100 mg/L
Solution Trial 1 Trial 2 Trial 3 Average Activity Index(Al) Plant Extract 18 19 20 19 2.17 Chloramphenicol 25 25 25 25 3.17
Batch culture time prediction: A strain of Escherichia coli has been genetically modified to produce human protein. A batch culture is started by inoculating 12 g of cells into a 100 liters bubble column fermenter containing 10 g/l glucose. The culture does not exhibit a phase lag. The maximum specific growth rate of the cells is 0.9 h^-1, the glucose biomass yield is 0.575 g/g. Estimate the time required to reach the stationary phase.
Hello! Can you please help me with the following: a. Draw the basic batch growth curve and label the four difference phases of growth and kinetics on the curve.b. Give a description of each phase on the curvec. Compare (less than, greater than, equal to, equal to zero) growth rate (Rg) and death rate (Rd) at each phase.d. What is the deceleration phase? Where is this phase on the curve (circle the location)?e. What does it mean that the exponential growth phase is 1st order? Please give the equation that represents the order of the exponential growth phase. Thank you!
Relative Concentration of Tea Extracts % Inhibition 0.25 0.974 0.5 0.967 0.75 0.965 1.5 0.959 0.25 0.858 0.5 0.976 0.75 0.982 1.5 0.969 0.25 0.974 plot the graph
10_UG_26563_ch09_167-184.indd 175 Press esc to exit full screen Yeast Fermentation The unicellular fungus Saccharomyces cerevisiae is used in the food industry to ferment sugars. The rate of fermentation is measured by CO₂ production. The addition of inorganic phosphate increases this rate. THINK Answer this question individually: 1. Why does the fermentation of sugars, such as glucose and fructose, depend upon the presence of P? PAIR Answer the following questions with a partner: 2. Which glycolytic reactions require P? 3. Several glycolytic intermediates contain phosphate. Where did these groups come from during the reactions? SHARE 4. How much P, is required to generate one CO₂? As a class, discuss the following questions: 5. Is CO₂ generation the most reliable way to measure ethanol fermentation? Why or why not? HANDOUT 175 23/03/17 2:02 pm Page 1 of 4 ...... ZOOM + K
Activity 2 You are required to culture and identified yogurt starter cultures, Lactobacillus bulgaricus and Streptococcus thermophilus from a diluted yogurt sample (1ml of yogurt was dissolved in 9ml of peptone water) and observed the growth rates of Streptococcus thermophilus under varying conditions. The standard media accepted by the International Dairy Federation of the yogurt species, L. bulgaricus and S. thermophilus are MRS and M17 agar, respectively. You are also required to carry out different staining and biochemical techniques to identify different medically important bacterial species apart from yogurt cultures. Produce a report that includes; Description of how you have carried out aseptic techniques to cultivate these microorganisms to obtain pure cultures of bulgaricus and S. thermophilus from the mixed population (explain the principles behind each aseptic techniques used) and confirmation of these bacterial species by performing Gram’s staining. Description of…
Test Tube # Enzyme 1 (uL added) Product Formation (umol) 1 0 0 2 25 0.08 3 50 0.15 4 75 0.22 5 100 0.3 6 125 0.37 7 150 0.45 8 175 0.53 9 200 0.59 10 225 0.68 11 250 0.74 A. Make a graph is Excel/Google Sheets plotting Product formation (umol) on the y-axis and Enzyme volume added on the x-axis. Paste the graph below. Make sure to have axis labels and a title on the graph. B. What can you conclude from this graph, ie how is the amount of product formed changing as a function of the amount of enzyme present?
Question: A farmer is growing grapes on a soil that has an available moisture capacity of 12%. The rooting depth of the crop is 4 ft. 1. Calculate the number of irrigation events and total amount of water required to irrigate the farmer’s 60-ac vineyard for two weeks considering the following: a. A maximum depletion level in the root zone of 15% b. Average ET of 0.2 in/day c. A system efficiency of 90%
l l l l M cell structure Cin chemostat is fed with sugar The growth rate follows the process equation VscS+C2C + Vp VPC о where P is the useful product The rate law followed is as determined experimentally rg = kg [C] [S] 1. (S) Orite down the mass balances for S, C, P and I, assuming feed contains ody the substrate of concentration [5]b] Solve them for [S], [C] and [P],
The yeast S.cerevisae is cultivated for ethanol production in a 10000 L fermenter operated continuously at steady state. The reactor is fed by a 50 g/L of sucrose, with a feed flow of 400 L/h. Assuming that the yeast growth kinetics follow the Monod model and using the following information:Data: μmax= 0.1h-1 ; Ks=0.5g/L; Yx/s=0.15 g/g; Yp/s=0.45g/g. a) What are the concentrations of biomass, sucrose and ethanol at the exit of the fermenter?b) Calculate the productivity in ethanol (Pp) and sketch the curve of variation of Pp vs. dilution rate.c) In terms of ethanol productivity, would it be more or less advantageous if the reactor were operated with a feed rate of 1200L/h? Justify.
3.1 Given these images focused under different objectives, calculate the size of an onion cell and a waterweed cell. Show your calculations. TREVISIONMIO viewed in HPO A. onion Size: Size: Calculation: mm um viewed in LPO B. waterweed Size: Size: Calculation: _mm um

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