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- Analysis of the pathological constituents GIVE THE FOLLOWING: a. Name of Test/Test Reagent b. Positive Result Blood (Note: Presence of blood in urine is termed as hemolobinuria) To 1 ml of freshly prepared Benzidine solution (sat’d sol’n of Benzidine in glacial Acetic acid) and 10 drops of urine sample. Add 5 drops of 3% H2O2. A positive result is indicated by a blue green color.a. Silver reduction test: What is the substance responsible for the color production? b. Jaffe’s test: To what is the color due to? c. Ammoniacal zinc chloride test: What compound is responsible for the color? What are the pigments normally present in urine? d. Sulfates: From what substance are sulfates derived? In what conditions is sulfate elimination increased? e. Detection of Chlorides: What is the normal amount of chlorides eliminated in 24 hours? In what forms are they eliminated? In what conditions is the elimination diminished and eliminated increased? What is a threshold substance? Is chloride a substance substance? f. Detection of Phosphates: What is a triple phosphate? When it is formed? What are the sources of the phosphates found in urine?The nurse is cleaning an open abdominal wound that hasunapproximated edges. What are accurate steps in thisprocedure? Select all that apply. a. Use standard precautions or transmission-based precau-tions when indicated. b. Moisten a sterile gauze pad or swab with the prescribedcleansing agent and squeeze out excess solution.c. Clean the wound in full or half circles beginning on theoutside and working toward the center.d. Work outward from the incision in lines that are parallel toit from the dirty area to the clean area. e. Clean to at least one inch beyond the end of the new dress-ing if one is being applied. f. Clean to at least three inches beyond the wound if a newdressing is not being applied.
- Explain how to do each of the following special tests and what they would indicate 1. Thomas TestList the procedure of the biuret testI. II. III. IV. 2 1 2 2 3 12pt ✓ Paragraph 3 2 3 4 3 Edit View Insert Format Tools Table BIU 4 A 5 6 5 Hemophilia If IV. 1 were to have a child with someone with no family history of either condition, what are the odds that their child would be afflicted with hemophilia? ✓ T² v Color blindness 6 ⠀ 6 O
- Determine the interfering agent for each of the following tests. A. Ascorbic B. Barium C. Antibiotics D. Zinc oxide E. None 1. Benzidine Test 2. Macroscopic stool analysis - Color 3. Gmelin's Test 4. Macroscopic stool analysis - AppearanceDetermine which reason/substance IS NOT directly related to the given urine analysis result. 1. Orange urine RBC Bilirubin Azo-gantrisin Pyridium 2. Black urine Melanin Homogentisic acid Melanogen Metabolite of phenylalanine 3. Strongly positive on Glucose (Reagent strip) D-Penicillamine Cecon Glycosuria Pregnancy 4. Weakly positive on blood (Reagent strip) Zonrox Chlorpromazine Hydrogen peroxide Hematuria 5. Pathologic milky urine Squamous epithelial cells RBC WBC Bacteria 6. Strongly positive for bilirubin (Reagent strip) Pyridium Chlorpromazine Chlorhexidine PhenazopyridineDetermine the positive results for each of the following tests. * B. Changes to green, blue, violet, yellow and A. Bloody- C. Clear liquid D. Blue-green E. Green streaked coloration stool mucus red Benzidine Test Macroscopic stool analysis - Color Gmelin's Test Macroscopic stool analysis - Appearance
- II. Tests for Lipids A. Bromine Test Table 3. Results for Bromine test. SAMPLE Ranking of the number of drops of Bromine Degree of unsaturation Oleic oil Coconut oil Soybean oil * Rank (1", 2nd, 3rd) – 1ª as the highest number of drops; 3d as the lowest number of drops ** minimal, moderate, highest B. Acrolein Test Table 4. Results for the Acrolein test. INFERENCE** SAMPLE Oleic oil Coconut oil Beef fat Egg yolk Pungent odor or no observable result * positive or negative for glycerol OBSERVATION c. Zak's Test Table 5. Results for Zak's test. SAMPLE OBSERVATION INFERENCE*. Oleic oil Coconut oil Egg yolk * Purple product or no observable result * positive or negative for cholesterol D. Saponification Table 6. Results for saponification. SAMPLE REMARKS* Soap suspension (Step 2) Purified soap (Step 3) Acid hydrolysis product of soap (Step 4) Rank (14, 2nd, 3rd) as to the estimated pH value -1 as the highest pH value; 3rd as the lowest pH valueDifferentiate bilirubin detection in the urine using reagent strip testing with the following tests in terms of sensitivity and specificity. a. Foams test b. Smith's test c. Harrison's test d. Ictotest TableWhat is a clinical specimen? b. Laboratory results obtained in the clinical laboratory are only as good as the quality of the clinical specimen collected for the laboratory analysis. Discuss this in the light of the precautions to ensure in collecting clinical specimen as a nurse/midwife. c. Explain any 2 methods of clinical specimen collection. d. Explain any 2 laboratory procedures or test that are performed on clinical specimen for laboratory investigations.