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A protein with a molecular weight of about 12,000 Da was isolated in elevated quantities from cells undergoing apoptosis. Researchers want to know what this protein is. It was easily purified and then was exposed to fragmentation with proteases and cyanogen bromide. The resulting pep- tides were separated on a C18 column on HPLC and then sequenced using Edman degradation. First, remind yourself of how these peptide sequences were sequenced by briefly describing how Edman degradation works. Next, examine the peptide lists that result from proteolysis (or fragmentation) of the unknown protein in handouts B through E. Use the peptide lists to compile the sequence of the protein. Your instructor may ask you to use two specific lists, three specific lists, or all of the lists. If/when you have access to the Internet, submit your final sequence to a database to identify the protein. For example, submit your sequence at the protein BLAST site of the National Center for Biotechnology Information (NCBI) by going to nih.gov and searching for "Basic Local Alignment Search Tool." What is the protein? Now, put the peptides in order by placing each one's ordinal (1st #1, 2nd #2, etc.) in the far-left column preceding each peptide sequence.

A protein with a molecular weight of about 12,000 Da was isolated in elevated quantities from cells undergoing apoptosis. Researchers want to know what this protein is. It was easily purified and then was exposed to fragmentation with proteases and cyanogen bromide. The resulting pep- tides were separated on a C18 column on HPLC and then sequenced using Edman degradation. First, remind yourself of how these peptide sequences were sequenced by briefly describing how Edman degradation works. Next, examine the peptide lists that result from proteolysis (or fragmentation) of the unknown protein in handouts B through E. Use the peptide lists to compile the sequence of the protein. Your instructor may ask you to use two specific lists, three specific lists, or all of the lists. If/when you have access to the Internet, submit your final sequence to a database to identify the protein. For example, submit your sequence at the protein BLAST site of the National Center for Biotechnology Information (NCBI) by going to nih.gov and searching for "Basic Local Alignment Search Tool." What is the protein? Now, put the peptides in order by placing each one's ordinal (1st #1, 2nd #2, etc.) in the far-left column preceding each peptide sequence.

Biochemistry
Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter30: Protein Synthesis
Section: Chapter Questions
Problem 1P
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This is the study of Biochemistry. This is all that was provided alongside the question. Please complete with explanations to the best of your ability 

Activity: De-Proteolyze This! Staphylococcal peptidase I peptides E YLE MGDVE DTLME Trypsin RADLIAYLKKATNE KGKKIFIMKCSQCHTVE NPKKYIPGTKMIFVGIKKKE Asp-N endopeptidase peptides KGGKHKTGPNLHGLFGRKTGQAPGYSYTAANKNKGIIWGE MGD LIAYLKKATNE TLMEYLENPKKYIPGTKMIFVGIKKKEERAD VEKGKKIFIMKCSQCHTVEKGGKHKTGPNLHGLFGRKTGQAPGYSYTAANKNKGIIWGED CNBr peptides M GDVEKGKKIFIM EYLENPKKYIPGTKM IFVGIKKKEERADLIAYLKKATNE KCSQCHTVEKGGKHKTGPNLHGLFGRKTGQAPGYSYTAANKNKGIIWGEDTLM K K K K K E HK NK GK EER GGK ATNE IFIMK YIPGTK MGDVEK MIFVGIK ADLIAYLK CSQCHTVEK TGPNLHGLFGR TGQAPGYSYTAANK GIIWGEDTLMEYLENPK
Transcribed Image Text:Activity: De-Proteolyze This! Staphylococcal peptidase I peptides E YLE MGDVE DTLME Trypsin RADLIAYLKKATNE KGKKIFIMKCSQCHTVE NPKKYIPGTKMIFVGIKKKE Asp-N endopeptidase peptides KGGKHKTGPNLHGLFGRKTGQAPGYSYTAANKNKGIIWGE MGD LIAYLKKATNE TLMEYLENPKKYIPGTKMIFVGIKKKEERAD VEKGKKIFIMKCSQCHTVEKGGKHKTGPNLHGLFGRKTGQAPGYSYTAANKNKGIIWGED CNBr peptides M GDVEKGKKIFIM EYLENPKKYIPGTKM IFVGIKKKEERADLIAYLKKATNE KCSQCHTVEKGGKHKTGPNLHGLFGRKTGQAPGYSYTAANKNKGIIWGEDTLM K K K K K E HK NK GK EER GGK ATNE IFIMK YIPGTK MGDVEK MIFVGIK ADLIAYLK CSQCHTVEK TGPNLHGLFGR TGQAPGYSYTAANK GIIWGEDTLMEYLENPK
A protein with a molecular weight of about 12,000 Da was isolated in elevated quantities from cells undergoing apoptosis. Researchers want to know what this protein is. It was easily purified and then was exposed to fragmentation with proteases and cyanogen bromide. The resulting pep- tides were separated on a C18 column on HPLC and then sequenced using Edman degradation. First, remind yourself of how these peptide sequences were sequenced by briefly describing how Edman degradation works. Next, examine the peptide lists that result from proteolysis (or fragmentation) of the unknown protein in handouts B through E. Use the peptide lists to compile the sequence of the protein. Your instructor may ask you to use two specific lists, three specific lists, or all of the lists. If/when you have access to the Internet, submit your final sequence to a database to identify the protein. For example, submit your sequence at the protein BLAST site of the National Center for Biotechnology Information (NCBI) by going to nih.gov and searching for "Basic Local Alignment Search Tool." What is the protein? Now, put the peptides in order by placing each one's ordinal (1st #1, 2nd #2, etc.) in the far-left column preceding each peptide sequence.
Transcribed Image Text:A protein with a molecular weight of about 12,000 Da was isolated in elevated quantities from cells undergoing apoptosis. Researchers want to know what this protein is. It was easily purified and then was exposed to fragmentation with proteases and cyanogen bromide. The resulting pep- tides were separated on a C18 column on HPLC and then sequenced using Edman degradation. First, remind yourself of how these peptide sequences were sequenced by briefly describing how Edman degradation works. Next, examine the peptide lists that result from proteolysis (or fragmentation) of the unknown protein in handouts B through E. Use the peptide lists to compile the sequence of the protein. Your instructor may ask you to use two specific lists, three specific lists, or all of the lists. If/when you have access to the Internet, submit your final sequence to a database to identify the protein. For example, submit your sequence at the protein BLAST site of the National Center for Biotechnology Information (NCBI) by going to nih.gov and searching for "Basic Local Alignment Search Tool." What is the protein? Now, put the peptides in order by placing each one's ordinal (1st #1, 2nd #2, etc.) in the far-left column preceding each peptide sequence.
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