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- Using Figures 8.7 and 8.9 as a guide, draw a dinucleotide composed of C and A. Next to this, draw the complementary dinucleotide in an antiparallel fashion. Connect the dinucleotides with the appropriate hydrogen bonds. FIGURE 8.9 The two polynucleotide chains in DNA run in opposite directions. The left strand runs 5 to 3, and the right strand runs 3 to 5. The base sequences in each strand are complementary. An A in one strand pairs with a T in the other strand, and a C in one strand is paired with a G in the opposite strand. FIGURE 8.7 Nucleotides can be joined together to form chains caled polynucleotides. Polynucleotides are polar molecules with a 5 end (at the phosphate group) and a 3 end (at the sugar group). An RNA polynucleotide is shown at the left, and a DNA polynucleotide is shown at the right.2. Predict the following sequences of base in the DNA strands complementary to the single RNA strand: • 3'A-G-T-C-G-T-A-C-A-T-G 5 • 5'G-T-C-G-T-A-C-T-A-G-C 3' • 5'C-A-T-G-C-T-A-G-C-T-A 3' • 3'A-T-C-A-T-G-C-G-T-A-C 5' • 5'T-G-A-C-G-T-A-C-G-A-T 35. Create the complimentary strand for the DNA strand below. Make sure to label the parts and direction of the strand. 5'-GGCAACGGTCCAGTCCAAGTTACG-3' 6. What are the amino acids coded for by this sequence of nucleotides: ATG GGA ACT CCA 7. What is the complementary messenger-RNA sequence for the DNA sequence shown below? ATC GGA CCG ATT GCC
- 13. a. Draw in the corresponding DNA nucleotide that would base pair with the adenine nucleotide shown below using the same level of detail as provided in the image below. Hint: Remember that DNA bases pair in the antiparallel orientation. орасна you A OH H b. How do you know the adenine nucleotide drawn above is from DNA and not RNA?1. Write the complementary base sequence for the matching strand in the following DNA section: -A-G-T-C-C-A-A-T-C–(üij If one of the strands of DNA has the following sequence of bases running in the 5' →3' direction 5'-G-G-A-C-A-A-T-C-T-G-C-3' 3'e c 4 GT TAGA C GS vhat base is 'closes: to 5' end in the compiementary strand?
- 1) You will start off by laying out your individual nucleotides for the first strand using the following sequence: A, C, G, T. 2. Using a pem or pemcil, indicate the phosphodiester bond that forms between each of the nucleotides. Look at figure 7 Label the 5' and 3' ends of the strand you just built. Identify and label one purine and one pyrimidine nitrogen base. 2. You are now ready to build your second strand. The second strand will be antiparallel. You will need to flip the nucleotides upside down to form hydrogen bonds between the nitrogen bases. Remember, A bonds to T through 2 hydrogen bonds and C to G with 3 hydrogen bonds. Draw in the hydrogen bonds with a chalk marker. Look at figure 8. Label the 5' and 3' ends of the second strand. Take a photo of your completed molecule and insert it into your lab notebook. 3. Explain dehydration synthesis, including what atoms are removed, and what molecule becomes a byproduct of these reactions.1. Determine what amino acid will be formed from the given DNA strand below: 3’ T A C A T G C C G A A T G C C 5’ 2. how will replication happen by mentioning the enzyme needed then transcribe to form mRNA. 3. Discuss what will happen to mRNA, then translate, mentioning the anticodon to be used 4. Look at the genetic code to know what amino acid will become part of the polypeptide chain.4. If your nucleotide sequence is - Is this sequence DNA or RNA? Explain how you know. What would the sequence of the complementary strand be? 5'- ATGTCCTAGAG - 3'
- 22..2 if one strand of the DNA molecule has the sequence 5’ TACGA 3, The other strand would have the sequence: 3’ UACGCA5’ 3’AUGCGU 5’ 3’ ATGCGT 5’ 3 TACGCA 5’ 3’ ATGCGT 5’3. On the diagram below... Using green dashed lines, draw in the hydrogen bonds on the right figure. Using the same color, circle the location of the H-bonds on the left figure. - Using a red pen, circle where the molecules have a negative charge on the right figure. Using the same color, color the area with the negative charge in the left figure. - Is this DNA or RNA? Explain two ways that you know. Clearly label the 5' and 3' ends so that you know which is which! This skill is VERY important. C A A CO G >0.34 nm 3.4 nm HO- HO-P-O O=PIO O=D HO-P-O OH H H O O N- N N N N H H N-H N N H-N H-N H Z-H N FN N 5-1=0 OH O-P-OH O O O-P-OH O -OH34. Give the sequence of the complementary DNA strand of the DNA chain with the following base sequence: 5’-TATGGCATAC-3’
