Ti plasmid

Results: From the results table we notice that on an average Amoxicillin is the most effective in inhibiting the growth of the strains of E.Coli bacteria with bleach coming in as second effective. In some cases Isocol had little or no effect to the bacteria culture growth, showing some results similar to the controlled variable. However on average it was still the least effective, even though like Dettol, Isocol is classified as an antiseptic chemical. In saying this however when looking at the

Many aspects of E. coli K1 systemic infection in neonates have been reproduced using animal models (10). In particular, the neonatal rat model mimics the natural path of infection: pups are orally administered E. coli K1, leading to colonization of the gastrointestinal tract, translocation across the intestinal epithelium, survival and replication in the bloodstream, and invasion of other organs (6, 10, 13). Furthermore, the model is highly-age dependent; whilst both two-day-old (P2) and nine-day

Resistant E. coli Cells Shalee Sommer Life 102 Lab section 32 December 2, 2015  Introduction: Certain cells when placed in certain environments or treatments the cell can take on genetic information passed on through phages or plasmids. (Weedman 2015) E. coli, or Escherichia coli, is a bacteria that resides in the colons of humans. (WebMD 2009) E. coli colonies usually perish in the presence of ampicillin a type of antibiotic designed to kill bacteria like E. coli. Heat shock is

One of the primary goals of this experiment is to generate a genomic library for A. fischeri, and the first step to do this involves isolating and analyzing the chDNA of the bacteria. This was completed using the Qiagen DNeasy Extraction Kit, which involves a variety of steps including: lysis of the chDNA, the binding of the sample to silica, multiple washings of the sample, and an elution to obtain pure chDNA for analysis (Qiagen, 2006). To start off, 180 μl’s of resuspension buffer was added to

Introduction In this experiment, LacZ DNA segment was isolated by PCR and ligated to pET32a vector plasmid for further use in future experiments. LacZ gene has been used in wide range of experiments in the Biochemistry field. The gene produces the -galactosidase enzyme that is malleable and easy to manipulate, making it a great subject for experiments to understand the molecular genetics of prokaryotes (Serebriiskii and Rose et al.). The LacZ gene was isolated from E.coli bacteria by PCR because

The E. Coli DNA Isolation and Analysis lab’s purpose was to isolate E. Coli DNA and analyze it using gel electrophoresis. We were able isolate E. Coli DNA and we could see the bacteria growing on our plates. Because of the way we streaked it across, We were able to see the long lines of the organisms, but also smaller colonies as the number dwindled on later sections of the plate. The salmon sperm DNA we gathered made a long, bright strip during gel electrophoresis, compared to the E. Coli, which

Streptococcus pneunomiae (SpHTS). The PCR result showed that there was no band in the DNA gel electrophoresis, which means the experiment was not successful. The transformation process presented that there were no colonies growing in the agar plate. The plasmid DNA had been purified and the concentration measured was 25.2 ng/μL. The restriction digest sample had two visible fragments at 3000 and 3600 base pairs. There were five fractions of protein purification, which indicated five different protein extraction

The hypothesis above tested the insertion of the pGlo gene to see if the bacteria, E.Coli, will reproduce and grow in the presence of ampicillin and to see if it will cause a green fluorescent glow. (PGLO™ Bacterial Transformation Kit,2017). Based upon the results from this experiment the hypothesis did support the hypothesis and that the presence of the pGlo gene inserted into the E.Coli did cause for growth and fora fluorescent glow to occur. In the experiment, the petri dishes that contained

To determine if the type of agar affects bacterial growth, a scientist cultures E. coli on four different types of agar. Five petri dishes are set up to collect results: One with nutrient agar and E. coli One with mannitol-salt agar and E. coli One with MacConkey agar and E. coli One with LB agar and E. coli One with nutrient agar but NO E. coli All of the petri dishes received the same volume of agar, and were the same shape and size. During the experiment, the temperature at which the petri

Date: April 24th, 2015 Introduction: Plasmids are small genetic molecules containing their own DNA that are able to replicate autonomously. They reside within bacteria with the capability of providing environmental advantages such as, antibiotic resistance, which would increase the bacteria’s environmental fitness if there were a threat of antibiotics. Genetic transformation is the introduction of a plasmid into a new cell. During this process, a foreign plasmid is introduced to a host cells DNA, allowing