Biology: Science for Life with Physiology (6th Edition) (Belk, Border & Maier, The Biology: Science for Life Series, 5th Edition)
6th Edition
ISBN: 9780134555430
Author: Colleen Belk, Virginia Borden Maier
Publisher: PEARSON
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Chapter 9, Problem 8LTB
Summary Introduction
Introduction:
DNA profile is a technique that is used to get information about a person's DNA. It is used in criminal investigations to detect the culprits. There are different types of DNA profiling such as RFLP and STR.
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Genetic Engineering Terms
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DNA sequencing
The use of an organism, or a
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copies of a specific DNA region
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than anorganism)
Recombinant DNA
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A technique used.to separatę DNA
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fragments from multiple sources
Biotechnology
A molecular biology technique that
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The process of determining the
sequence of nucleotides (As, Ts,
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tİ6111749/enetic-engineering-tems
DNA fingerprinting uses a process called gel electrophoresis to separate the fragments of DNA. Once the DNA fragments are sorted, the pattern of bands can be analyzed.
Gel Electrophoresis Procedure
The smaller DNA fragments start to move away from the wells and the larger DNA fragments remain closer to the wells.
An electric current is passed through the gel.
DNA fragments are treated with a dye.
A restriction endonuclease is added to the DNA.
Using micropipettes, the DNA samples are added to the wells.
DNA fingerprint is produced.
DNA fragments are produced.
The order in which a DNA fingerprint is produced using gel electrophoresis is Answer, Answer, Answer, Answer, Answer, Answer, and Answer.
DNA EXTRACTION
Materials:
knife 1 cup of fruit
Table salt clean piece of cloth or strainer for filtering
resealable bag dishwashing liquid
70% rubbing alcohol (chilled) shot glass (or any transparent and narrow container resembling a test tube)
How to do the extraction:
Before you begin, make sure you have chilled your alcohol in the freezer for at least 3 hours. Room temperature alcohol will not work nearly as well as cold alcohol. Place about 50 mL of alcohol in the freezer to chill it and take it out only when you’re going to need it. (The alcohol will not actually freeze.)
1. The first thing you will need is a sample. Since DNA is found in all living…
Chapter 9 Solutions
Biology: Science for Life with Physiology (6th Edition) (Belk, Border & Maier, The Biology: Science for Life Series, 5th Edition)
Ch. 9 - Is a round yellow pea seed (genotype Rr Y y) an...Ch. 9 - What factors cause quantitative variation in a...Ch. 9 - The DNA profile below is from a mother, a father,...Ch. 9 - Prob. 4LTBCh. 9 - Prob. 5LTBCh. 9 - Prob. 6LTBCh. 9 - Prob. 7LTBCh. 9 - Prob. 8LTBCh. 9 - Add labels to the figure that follows, which...Ch. 9 - Prob. 10LTB
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- Describe how each of the following is used DNA hybridization. Polymerase chain reaction (PCR) . DNA sequencing Probe labelingarrow_forwardGel Electrophoresis is used in many different forms to learn about DNA, RNA or proteins. Research one laboratory method or technique that uses DNA electrophoresis in order to learn more or make determinations about DNA, RNA or Proteins. Name of technique or method Brief description of what the electrophoresis results are used for in the method. Include a link to your resources Include an image of the results or technique you describearrow_forwardRegarding STR markers used in forensic science. Tick all the correct statements: no correct statement the PCR primers used to amplify STRs are located in the repeat units PCR primers to amplify STRs are located on both sides of the repeat units the PCR primers used to amplify STRs are coupled to a fluorochrome which is essential for the detection of amplicons they are absent from the gonosomes the allelic frequencies of STR markers vary according to the ethnicity of the individuals genotyped they are present homogeneously throughout the nuclear genomearrow_forward
- Which of the following is/are true regarding a PCR reaction performed to amplify a DNA plasmid? There may be more than one correct answer; select all that apply. Two primers are needed to facilitate double stranded DNA extension. Denaturation, when the primers bind to the DNA template, is performed at the highest temperature. Extension is the step when the polymerase catalyzes nucleotide incorporation during polymerization. ATP, UTP, GTP, and CTP are all required for polymerization.arrow_forwardWhich is a complete list of the ingredients that are essential for PCR? nucleotides, DNA template, Taq polymerase, and plasmids nucleotides, DNA template, DNA ligase, and plasmids nucleotides, DNA template, Taq polymerase, and primers restriction enzymes, DNA template, Taq polymerase, and primers nucleotides, DNA template, DNA ligase, and primersarrow_forwardWhich of the following is NOT a tool used in recombinant DNA technology? Cutting specific DNA sequences using restriction enzymes. Adding Taq polymerase to copy DNA in PCR. Using primase to catalyze the formation of phosphodiester bonds. Inserting a gene of interest into a vector. Inserting a plasmid into bacteria to generate copies.arrow_forward
- What molecular biology technique is used to amplify DNA from an evidence sample? Reverse dot blotting Southern hybridization Cloning Polymerase chain reaction (PCR) Capillary electrophoresisarrow_forwardHumans have very similar DNA sequences, with approximately 999/1000 letters being ide One possible way of telling the difference between these very similar sequences is by: Genetic engineering Analyzing RFLPS using gel electrophoresis Comparing the total amount of DNA from two or more peoplearrow_forwardRECOMBINANT DNA BRIEFLY, DESCRIBE RECOMBINANT DNA AND GIVE ONE CONCRETE EXAMPLE. EVALUATE THE SIGNIFICANCE/PRACTICAL APPLICATIONS OF THIS DNA TECHNOLOGY BY CONSIDERING ETHICAL AND MORAL IMPLICATIONS BEHIND IT. RECOMBINANT DNA EXAMPLE: MODIFIED TRAIT GENE MODIFICATION RECIPIENT ORGANISM FIELDS OF APPLICATION ALSO, WHAT ARE YOUR INSIGHTS? IN 2 TO 3 SENTENCES.arrow_forward
- Which of the following ARE part of a typical PCR reaction mixture? DNA ligase dNTPs (mix of nucleoside tri-phosphates) RNA primers made by primase enzyme template DNA, often from cells collected from hair, cheek swab, or blood 2 DNA primers polymerase enzymearrow_forwardSelect the statement below that is FALSE regarding DNA and DNA Fingerprinting: Select an answer and submit. For keyboard navigation, use the up/down arrow keys to select an answer. DNA fingerprints can be used to link remains to a particular person. a b Restriction enzymes cut molecules into fragments of identical lengths. Restriction enzymes cut molecules of DNA into a unique set of fragment length determined by the genetic code. d Subunits of DNA are called bases and include (adenine/guanine/ cytosine/ and thymine)arrow_forward1 2 Today's technology has made it easier to quickly and accurately generate DNA profiles. In this part of the activity, you will model the process yourself to solve a crime. Good luck, detective! Crime Report: A thief has stolen a priceless collection of jewels from the Museum of Precious Jewels. Forensic technicians obtained skin cells from a forehead print left on the glass enclosure of the jewel exhibit. DNA has been isolated and PCR amplified for some of the standard STR loci. A partial genetic profile generated from the collected DNA is shown in Figure 5. 10 50 DNA Profile from Forehead Print Number of base pairs 00 50 40 D58818 075830 I 16 MU DES1179 Shandand (10) 70 CSF1PO DITS820 80 100 Figure 5. The DNA profile of the forehead print from the scene of the crime. Each colored line shows the alleles for one of four of the core CODIS STR loci (D5S818, CSF1PO, D7S820, D8S1179). and data for the four STR loci that were included in the A suspect was identified in the case. Her DNA…arrow_forward
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