ANATOMY+PHYSIOLOGY
4th Edition
ISBN: 9781260265217
Author: McKinley
Publisher: RENT MCG
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Textbook Question
Chapter 8.2, Problem 5WDYL
What bones form the middle cranial fossa, and which part of the brain resides in this fossa
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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 8 Solutions
ANATOMY+PHYSIOLOGY
Ch. 8.1 - What is the general function of the axial...Ch. 8.1 - What is the difference between a foramen and a...Ch. 8.2 - Prob. 3WDYLCh. 8.2 - Prob. 4WDYLCh. 8.2 - What bones form the middle cranial fossa, and...Ch. 8.2 - Prob. 6WDYLCh. 8.2 - Prob. 7WDYLCh. 8.2 - In which four bones are the paranasal sinuses...Ch. 8.3 - Prob. 9WDYLCh. 8.4 - Prob. 10WDYL
Ch. 8.4 - What are the two largest fontanelles, and when do...Ch. 8.5 - Prob. 12WDYLCh. 8.5 - What are the secondary curves, and when do they...Ch. 8.5 - Compare the locations and functions of the...Ch. 8.5 - Prob. 15WDYLCh. 8.6 - Prob. 16WDYLCh. 8.6 - Prob. 17WDYLCh. 8.7 - Prob. 18WDYLCh. 8.8 - How do the sternal end and acromial end of the...Ch. 8.8 - Prob. 20WDYLCh. 8.9 - Prob. 21WDYLCh. 8.9 - Prob. 22WDYLCh. 8.9 - Prob. 23WDYLCh. 8.9 - Describe how the radius and ulna are positioned...Ch. 8.9 - Prob. 25WDYLCh. 8.10 - What three bones fuse to form the os coxae?Ch. 8.10 - Prob. 27WDYLCh. 8.10 - How is the pelvic inlet distinguished from the...Ch. 8.10 - Prob. 29WDYLCh. 8.10 - What are some differences in the symphysial...Ch. 8.11 - Prob. 31WDYLCh. 8.11 - Prob. 32WDYLCh. 8.11 - What are some bony features that are similar or...Ch. 8.11 - Prob. 34WDYLCh. 8.11 - Prob. 35WDYLCh. 8.11 - Prob. 36WDYLCh. 8.12 - Prob. 37WDYLCh. 8 - Prob. 1DYKBCh. 8 - Which bone marking is matched with its correct...Ch. 8 - The frontal and parietal bones articulate at the...Ch. 8 - Prob. 4DYKBCh. 8 - Prob. 5DYKBCh. 8 - Prob. 6DYKBCh. 8 - Prob. 7DYKBCh. 8 - Prob. 8DYKBCh. 8 - The femur articulates with the tibia at the a....Ch. 8 - Prob. 10DYKBCh. 8 - Prob. 11DYKBCh. 8 - Prob. 12DYKBCh. 8 - What are the functions of the paranasal sinuses?Ch. 8 - Prob. 14DYKBCh. 8 - Describe similarities and differences among true,...Ch. 8 - Compare and contrast the anatomic and functional...Ch. 8 - What are the primary similarities and differences...Ch. 8 - Prob. 18DYKBCh. 8 - Prob. 19DYKBCh. 8 - Prob. 20DYKBCh. 8 - Prob. 1CALCh. 8 - Prob. 2CALCh. 8 - Prob. 3CALCh. 8 - Prob. 4CALCh. 8 - Use the following paragraph to answer questions...Ch. 8 - Prob. 1CSLCh. 8 - Prob. 2CSLCh. 8 - Forensic anthropologists are investigating...
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- Which of the following is not a strength of using 16S rRNA for phylogenetic analyses? OA. It's cheap OB. It's easy to do C. It can be used to identify all the way down to the strain level OD. Both A & B OE. None of the abovearrow_forwardWhy are molecular approaches important to the field of microbial taxonomy and phylogeny? Phylogenetic inferences based on molecular approaches provide the most robust analysis of microbial evolution currently available. It allows for the collection of a large and accurate dataset from many organisms Almost no fossil record was left by microbes when compared to plants and animals All of the above None of the abovearrow_forwardYou will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. You have already cultured it and gone through the plate isolation procedure. Before you ship your samples off for sequencing, you want to do one final check of the A260 ratios. You get back the following ratios: A260/280 ratio is 1.89; A260/230 is 2.01. These ratios are close enough to the accepted "pure" values so they could be considered "pure" and mostly (if not completely) free of contaminants from the PCR process. True Falsearrow_forward
- You will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. After receiving your sequence back from the sequencing lab, you feel that you have, in fact, discovered and isolated a new species. You ask a fellow labmate about how you should proceed, and he tells you the following is the proper way to introduce a new species for recognition: Cultures have to be sent to international culture collections. Then a paper must be published describing the new organism and providing a genus and species name. You recall learning about this in your Microbiology course in college. Is this information from your colleague true or false? True Falsearrow_forwardis often a good indication of phylogenetic relatedness in phenotypes. Life-cycle patterns Cleavage patterns O Gene expression O Morphological similarityarrow_forwardWhich of the following is a weakness of using 16S rRNA for phylogenetic analyses? It can only go down to the family and genus levels It takes months to complete O Both of the above O None of the abovearrow_forward
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