Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
12th Edition
ISBN: 9780134605203
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Chapter 8, Problem 5Q
Summary Introduction
To determine:
The reason for not using heat in Kinyoun modification.
Introduction:
The acid-fast stain is used to differentiate between acid-fast bacteria and non-acid fast bacteria. The stain here is used in carbol fuchsin, which is soluble in the lipoidal material found in the mycobacterial cell wall. The decolorizing agent used here is acid alcohol.
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Chapter 8 Solutions
Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
Ch. 8 - What are the large blue-stained areas on the...Ch. 8 - What is the decolorizing agent in the Gram stain?...Ch. 8 - Prob. 3QCh. 8 - What is phenol (carbolic acid), and what is its...Ch. 8 - Prob. 5QCh. 8 - How might the acid-fast characteristic of...Ch. 8 - In 1882, after experimenting with staining...Ch. 8 - Clinical specimens suspected of containing...Ch. 8 - The acid-fast stain is used to detect...
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- Both crystal violet and safranin are basic stains and may be used to do simple stains on Gram positive and negative cells. This being the case, explain how they end up staining Gram positive and gram negative cells differently.arrow_forwardCrystal violet is an example of what type of stain?arrow_forwardThe bacterial cells in the image below were stained with a high concentration of carbolfuschin for an extended period of time (5 minutes) while the slide was heated. The slide was decolorized with a mixture of acid and alcohol. The slide was then counter-stained with methylene blue. What kind of stain does this describe?arrow_forward
- Which of the following is/are true regarding the acid-fast stain? (There may be more than one correct answer.) Non-acid-fast microbes appear blue in a completed acid-fast stain. O Acid-fast cells retain the primary dye after treatment with acid-alcohol. O It is used to identify members of the genus Mycobacterium. V Acid-fast cells appear bright pink/red in a completed acid-fast stain. O If cells are acid-fast, they are gram-negative.arrow_forwardWhy is it called as differential stainarrow_forward(please answer all)arrow_forward
- Plating 0.1 mL of a sample diluted by a factor of 10^(-3) produced 43 colonies. What was the original concentration (OCD) in the undiluted sample?arrow_forwardWhat is the primary stain for the Ziehl-Neelson acid fast stain? What color is this stain? What cells will display this stain once you finish the stain and view your slide? Paragraph BIUarrow_forwardExplain the mechanism of staining for each of the stains used. Iodine Methyl green Nigrosinarrow_forward
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