Microbiology with Diseases by Taxonomy (5th Edition)
Microbiology with Diseases by Taxonomy (5th Edition)
5th Edition
ISBN: 9780134019192
Author: Robert W. Bauman Ph.D.
Publisher: PEARSON
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Chapter 8, Problem 1MC

Which of the following statements is true concerning recombinant DNA technology?

  1. a. It will replace biotechnology in the future.
  2. b. It is a single technique for genetic manipulation.
  3. c. It is useful in manipulating genotypes but not phenotypes.
  4. d. It involves modification of an organism's genome.
Expert Solution & Answer
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Summary Introduction

Introduction:

Recombinant DNA technology is the technique of manipulation of the genome of microorganisms. It involves the cloning of genes through the use of restriction enzymes which cut DNA at specific sites in the genome.

Answer to Problem 1MC

Correct answer:

The recombinant DNA technology involves the modification of the genome of organisms.

Therefore, option (d) is correct.

Option (d) is given as “It involves modification of an organism’s genome”.

Explanation of Solution

Justify the reasons for the correct statement:

The recombinant DNA technology utilizes the restriction enzymes which can clone gene sequences inside vectors. These genes can then be expressed in the genome by injecting the plasmid inside the mammalian cells or bacterial cells.

Hence, option (d) is correct.

Justify the reasons for the incorrect statements:

Option (a) is given as “It will replace biotechnology in near future”.

Recombinant DNA technology and genetic engineering are branches of biotechnology. It is the use of genetic engineering principles used to make recombinant food products like cheese, butanol, and antibiotics. Hence, it is a wrong answer.

Option (b) is given as “It is a single technique for genetic manipulation”.

There are other techniques of genetic manipulation are electroporation and gene gun method. Hence, it is a wrong answer.

Option (c) is given as “It is useful in manipulating genotypes but not phenotypes”.

Genotypes and phenotypes regulation occurs through genes in the genome. Hence, it is a wrong answer.

Hence, options (a), (b), and (c) are incorrect.

Conclusion

The recombinant DNA technology utilizes restriction enzymes and modifies the genome of microorganisms according to the gene which is required to be expressed. This gene is cloned inside the vector for expression of the protein inside the cell.

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Which of the following is true about DNA manipulation? Select the best answer. A. all of these B. DNA has never been transferred between different organisms. C. Transferring DNA from one organism into another always kills the host organism. D. Whole genomes have been manipulated by humans for centuries; now individual genes ca be inserted into different organisms using a variety of techniques. E. DNA can't be manipulated.
Which statement is true?   a. There is no danger involved in recombinant DNA research in humans.   b. Stringent safety rules make the use of recombinant DNA research impossible.   c. There is no danger in releasing recombinant organisms into the environment.   d. Stringent safety rules make the use of recombinant DNA research possible.   e. There is no danger involved in recombinant DNA research in bacteria.
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