Campbell Biology: Concepts & Connections (9th Edition)
9th Edition
ISBN: 9780134296012
Author: Martha R. Taylor, Eric J. Simon, Jean L. Dickey, Kelly A. Hogan, Jane B. Reece
Publisher: PEARSON
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Chapter 8, Problem 19TYK
Bacteria are able to divide on a faster schedule than eukaryotic cells. Some bacteria can divide every 20 minutes, while the minimum time required by eukaryotic cells in a rapidly developing embryo is about once per hour, and most cells divide much less often than that. State at least two testable hypotheses explaining why bacteria can divide at a faster rate than eukaryotic cells.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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oxygen is terminal electron acceptor
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Chapter 8 Solutions
Campbell Biology: Concepts & Connections (9th Edition)
Ch. 8 - Complete the following table to compare mitosis...Ch. 8 - If an intestinal cell in a grasshopper contains 24...Ch. 8 - Which of the following is not a function of...Ch. 8 - It is difficult to observe individual chromosomes...Ch. 8 - A fruit fly somatic cell contains 8 chromosomes....Ch. 8 - If a fragment of a chromosome breaks off and then...Ch. 8 - Which of the following phases of mitosis is...Ch. 8 - A biochemist measured the amount of DNA in cells...Ch. 8 - A micrograph of a dividing cell from a mouse...Ch. 8 - Cytochalasin B is a chemical that disrupts...
Ch. 8 - Why are individuals with an extra chromosome 21,...Ch. 8 - In the light micrograph below of dividing cells...Ch. 8 - An organism called a plasmodial slime mold is one...Ch. 8 - Prob. 14TYKCh. 8 - Discuss the factors that control the division of...Ch. 8 - Compare cytokinesis in plant and animal cells. In...Ch. 8 - Sketch a cell with three pairs of chromosomes...Ch. 8 - Suppose you read in the newspaper that a genetic...Ch. 8 - Bacteria are able to divide on a faster schedule...Ch. 8 - Prob. 20TYKCh. 8 - A mule is the offspring of a horse and a donkey. A...Ch. 8 - What you think of as a banana is a Cavendish, one...Ch. 8 - SCIENTIFIC THINKING The study described in Module...
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- You will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. You have already cultured it and gone through the plate isolation procedure. Before you ship your samples off for sequencing, you want to do one final check of the A260 ratios. You get back the following ratios: A260/280 ratio is 1.89; A260/230 is 2.01. These ratios are close enough to the accepted "pure" values so they could be considered "pure" and mostly (if not completely) free of contaminants from the PCR process. True Falsearrow_forwardYou will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. After receiving your sequence back from the sequencing lab, you feel that you have, in fact, discovered and isolated a new species. You ask a fellow labmate about how you should proceed, and he tells you the following is the proper way to introduce a new species for recognition: Cultures have to be sent to international culture collections. Then a paper must be published describing the new organism and providing a genus and species name. You recall learning about this in your Microbiology course in college. Is this information from your colleague true or false? True Falsearrow_forwardis often a good indication of phylogenetic relatedness in phenotypes. Life-cycle patterns Cleavage patterns O Gene expression O Morphological similarityarrow_forward
- Which of the following is a weakness of using 16S rRNA for phylogenetic analyses? It can only go down to the family and genus levels It takes months to complete O Both of the above O None of the abovearrow_forwardAn unrooted tree containing ten unrelated species can become rooted by adding a descendant group related to two of the species. an unrelated outgroup. O a distantly related outgroup. O a descendant related to only one of the species.arrow_forwardWhat is the most appropriate purpose of building a phylogenetic tree? They look awesome You can use a tree to compare morphological characteristics of organisms It can be used to establish and analyze evolutionary relationships between species All of the abovearrow_forward
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