Prescott's Microbiology
10th Edition
ISBN: 9781259281594
Author: Joanne Willey, Linda Sherwood Adjunt Professor Lecturer, Christopher J. Woolverton Professor
Publisher: McGraw-Hill Education
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Chapter 7.8, Problem 4RIA
For each of the following, which enumeration technique would you use? Explain your choice. (a) A pure culture of Staphylococcus aureus; (b) a water sample that needs to be checked for E. coli contamination; (c) a sample of yogurt.
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You spread 0.1 mL volume of a 10^(-6) dilution onto a nutrient agar plate. After 24 hours of incubation at 37°C, there were 280 colonies of bacteria on the plate. A.) What is the original concentration (OCD) of bacteria in the stock sample this dilution came from? B.) Using the OCD value from part A, determine the number of colonies that would be expected to grow on a plate that is inoculated with 0.1 mL volume of 10^(-8) dilution from this same stock of bacteria. Show your work for both.
You spread 0.1 mL volume of a 10^(-6) dilution onto a nutrient agar plate. After 24 hours of incubation at 37°C, there were 280 colonies of bacteria on the plate. A.) What is the original concentration (OCD) of bacteria in the stock sample this dilution came from? B.) Using the OCD value from part A, determine the number of colonies that would be expected to grow on a plate that is inoculated with 0.1 mL volume of 10^(-8) dilution from this same stock of bacteria.
You are given a mix culture of S. aureus,E. coli and P. aeruginosa. Besides the streak plate method what other methods could you use to separate the bacteria? Please state what method(s) you would use, the result you would be looking for to help identify each bacterium and the interpretation of the result.
Chapter 7 Solutions
Prescott's Microbiology
Ch. 7.1 - MICRO INQUIRY In addition to chromosomes, what...Ch. 7.2 - MICRO INQUIRY Why is it important that the origin...Ch. 7.2 - MICRO INQUIRY What would be the outcome if FtsZ...Ch. 7.2 - MICRO INQUIRY Which step in the development of...Ch. 7.2 - Retrieve, Infer, Apply 1. Describe the three...Ch. 7.2 - Retrieve, Infer, Apply 2. How does the bacterial...Ch. 7.2 - Retrieve, Infer, Apply 3. Do you think MinCDE...Ch. 7.2 - Retrieve, Infer, Apply 4. Do you think Spiroplasma...Ch. 7.3 - What elements of the Sulfolobus spp. cell cycle...Ch. 7.3 - Many archaea have genes encoding an FtsZ...
Ch. 7.4 - What is the difference between halophilic and...Ch. 7.4 - Why do facultative anaerobes grow best at the...Ch. 7.4 - Retrieve, Infer, Apply 1. How do microorganisms...Ch. 7.4 - Retrieve, Infer, Apply 2. Define water activity...Ch. 7.4 - What are halophiles and why do Halobacterium spp....Ch. 7.4 - Retrieve, Infer, Apply 1. Define pH, acidophile,...Ch. 7.4 - Retrieve, Infer, Apply Classify each of the...Ch. 7.4 - Retrieve, Infer, Apply 3. Describe the mechanisms...Ch. 7.4 - Retrieve, Infer, Apply 1. What are cardinal...Ch. 7.4 - Prob. 3.2RIACh. 7.4 - Define psychrophile, psychrotolerant, mesophile,...Ch. 7.4 - Retrieve, Infer, Apply What metabolic and...Ch. 7.4 - Retrieve, Infer, Apply Describe the five types of...Ch. 7.4 - Retrieve, Infer, Apply What are the toxic effects...Ch. 7.4 - Retrieve, Infer, Apply Where would you expect to...Ch. 7.4 - Retrieve, Infer, Apply List the types of...Ch. 7.4 - Prob. 5.3RIACh. 7.4 - Prob. 5.4RIACh. 7.5 - MICRO INQUIRY What biomolecules make up the...Ch. 7.5 - Prob. 1RIACh. 7.5 - Prob. 2RIACh. 7.5 - Retrieve, Infer, Apply What is quorum sensing?...Ch. 7.5 - Retrieve, Infer, Apply How is the communication...Ch. 7.6 - Prob. 1RIACh. 7.6 - What are peptones, yeast extract, beef extract,...Ch. 7.6 - Retrieve, Infer, Apply Describe four ways in which...Ch. 7.6 - What are pure cultures and why are they important?...Ch. 7.6 - Retrieve, Infer, Apply It is known that microbial...Ch. 7.6 - Retrieve, Infer, Apply How might an enrichment...Ch. 7.7 - MICRO INQUIRY Identify the regions of the growth...Ch. 7.7 - Retrieve, Infer, Apply Define microbial growth.Ch. 7.7 - Retrieve, Infer, Apply Describe the phases of the...Ch. 7.7 - Why would cells that are vigorously growing when...Ch. 7.7 - Retrieve, Infer, Apply Contrast and compare the...Ch. 7.7 - Calculate the growth rate constant and generation...Ch. 7.7 - Suppose the generation time of a bacterium is 90...Ch. 7.8 - Why is it important to have no more than about 250...Ch. 7.8 - Briefly describe each technique by which microbial...Ch. 7.8 - Prob. 2RIACh. 7.8 - Prob. 3RIACh. 7.8 - For each of the following, which enumeration...Ch. 7.9 - Prob. 1MICh. 7.9 - Prob. 1RIACh. 7.9 - Prob. 2RIACh. 7.9 - Prob. 3RIACh. 7 - As an alternative to diffusible signals, suggest...Ch. 7 - If you wished to obtain a pure culture of bacteria...Ch. 7 - Design an experiment to determine if a...Ch. 7 - Suggest one specific mechanism underlying the...Ch. 7 - Consider cell-cell communication: bacteria that...Ch. 7 - Suppose you discovered a new bacterial strain from...Ch. 7 - Because many persistent bacterial infections...
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- You are testing a river water sample. Your "original" plate shows 100 coliform colonies. Assuming you did the serial dilution correctly, how many coliform colonies would you expect to see on your 1:10 plate? Your 1:100 plate? Explain.arrow_forwardGiven the scenario, compute for the total volume of the culture media solution (milliliter or liter) and dehydrated media (grams). Scenario: The students of a Microbiology class were tasked to transfer or subculture a pure culture of Escherichia coli bacterium in five 7 mL nutrient broth and five petri dishes of nutrient agar with 20 mL capacity each. Based on the instruction bottles for nutrient broth and nutrient agar, preparation of the culture media is as follows. Nutrient broth: 8 g/liter Nutrient agar: 28 g/liter Formula: C1V1 = C2V2 *Concentration *Volume Computation: What are the answers to the following. Weight in grams of nutrient broth: _________ Distilled water in mL for nutrient broth: __________ Weight in grams of nutrient agar __________ Distilled water in mL for nutrient agar: ____________arrow_forwardYou are given a 1 gram soil sample of unknown bacterial load. After doing 10-fold serial dilutions of the soil in sterile water, 100 uL volumes are taken from each dilution for preparation of pour plates. Following incubation, each half of the 10-8 plate has 46 colonies.a) What was the dilution factor?b) How many bacteria were present in the soil?2. Staphylococcus aureus divides every 20 minutes. A culture begins with 10 bacterial cells.a) After 5 hours, how many generations have occurredb) After 5 hours, how many bacteria are present?3. How many milliliters would you need to prepare a 10-2 dilution from a 10ml starting culture?arrow_forward
- 1) based on the difference in appearance between mixed culture streak plates and broth culture plates, what would lead you to believe that you have created a pure culture when you inoculated the broths? 2) when using the loop dilution technique to produce pour plates, did all the plates produce isolated colonies? If your goal was to ultimately create pure cultures of E. coli, M. luteus, and S. morcescens, which plate or which dilution would you use, and why would you use this plate? 3) What are the advantages and disadvantages of streak plating compared with pour plating?arrow_forwardyou are given a mixed culture of s. aureus, E. coli and P. aeruginosa. how would you isolate each other from this mixed culture?( Besides using plate technique)arrow_forwardIn the Harada-Mori culture technique, how are you going to dispose the culture tubes? Is it suitable to use refrigerated samples for this procedure Why or Why not?arrow_forward
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- Per the USDA, whole, unpasteurized fresh eggs can contain no more than 50,000 CFU/mL bacteria in a standard plate count. You are curious if the fresh eggs that you buy from your neighbor are considered safe to consume so you use your eScience Microbiology kit to test these eggs using direct plate count after serial dilution. After you complete the experiment, you obtain 74 countable colonies from the 10-2 dilution plate. The inoculum volume you plated was 0.1 mL. How many bacteria are present in 1 mL of the egg you sampled? Are these eggs considered safe to consume per USDA standards?arrow_forwardJoseph, being a know-it-all, told Mary that her method would not include all microbes. To demonstrate this, he took another 10g sample of Mary's garden and added starch. After the 1- week incubation time, he performed the same serial dilution scheme as Mary but used pour plating instead of spread plating. Shown below are the counts that he obtained after the incubation period. Compute for the CFU/g given the data below. Be sure to show complete solutions and box your final answer. Final answers should be expressed in 3 significant figures. Tube 1 135, 129 Tube 2 9.7arrow_forwardYou are interested in obtaining Staphylococcus aureus for a study investigating the prevalence of methicillin-resistant Staphylococcus aureus in the general population. You have received several samples and are ready to start your isolation procedures ,State if you use general or selective media and which specific media you would choose. How would you determine if the culture was contaminatedarrow_forward
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