Concept explainers
(a)
To explain: The order of amino acids Arg, His, and Leu that eluted from carboxymethyl column at pH 6.
Concept introduction: Separation and purification of biological macromolecules are done using carboxymethyl columns. These columns have carboxymethyl
(b)
To explain: The order of amino acids Glu, Lys, and Val that are eluted from diethylaminoethyl column at pH 8.
Concept introduction: DEAE (Diethylaminoethyl) cellulose chromatography is an ion exchange chromatography used for the separation and purification of proteins. DEAE cellulose is a weak anion exchanger, so the negatively charged protein binds to DEAE. It separates the substance based on their charges, where a negatively charged protein binds with the positively charged DEAE.
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Chapter 5 Solutions
Fundamentals of Biochemistry: Life at the Molecular Level
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