BIOLOGY
12th Edition
ISBN: 9781260169614
Author: Raven
Publisher: RENT MCG
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Chapter 5, Problem 1S
figure 5.5 describes a classic experiment demonstrating the ability of proteins to move within the plane of the cell’s plasma membrane. The following table outlines three different experiments using the fusion of labeled mouse and human cells.
| Experiment Conditions | Temperature (°C) Result |
| 1 Fuse human and mouse cells | 37 Intermixed membrane proteins |
| 2 Fuse human and mouse cells in presence of ATP inhibitors | 37 Intermixed membrane proteins |
| 3 Fuse human and mouse cells | 4 No intermixing of membrane proteins |
What conclusions can you reach about the movement of these proteins?
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Experiment I: Scientists took two types of human epithelial cells and placed them in an experimental
system that could measure intracellular (IN) and extracellular concentration (OUT) of 3 substances: Na+,
ATP and Glucose. Cell type A was nonmutant whereas cell type B had a mutation in a transmembrane
protein. At the beginning, middle and end of the experiment, concentrations were measured for all three
substances as shown below.
Cell type A (non-mutant)
Time
IN [Na+]
0
10 ms
20 ms
Concentration in mM
200
150
100
150mM
25mM
150mM
Q1. Analyze the intracellular concentration changes in the table (above) and the graph (below left).
Label the key at the bottom left. Graph the extracellular concentration changes in this data set using the
graph below right and the same key. With cell image on bottom right, diagram what is happening using
arrows and labels for the transported substance. And finally, at the bottom of the page describe what is
happening using words i.e. powers the movement of…
Chapter 5 Solutions
BIOLOGY
Ch. 5.1 - Prob. 1LOCh. 5.1 - Explain the fluid mosaic model of membrane...Ch. 5.2 - List the different components of phospholipids.Ch. 5.2 - Prob. 2LOCh. 5.2 - Prob. 3LOCh. 5.3 - Illustrate the functions of membrane proteins.Ch. 5.3 - Prob. 2LOCh. 5.3 - Prob. 3LOCh. 5.4 - Compare simple diffusion and facilitated...Ch. 5.4 - Prob. 2LO
Ch. 5.4 - Prob. 3LOCh. 5.5 - Prob. 1LOCh. 5.5 - Prob. 2LOCh. 5.5 - Prob. 3LOCh. 5.6 - Distinguish between endocytosis and exocytosis.Ch. 5.6 - Illustrate how endocytosis can be specific.Ch. 5 - According to the fluid mosaic model, membranes are...Ch. 5 - Prob. 2IQCh. 5 - The fluid mosaic model of the membrane describes...Ch. 5 - What chemical property characterizes the interior...Ch. 5 - The transmembrane domain of an integral membrane...Ch. 5 - The specific function of a membrane within a cell...Ch. 5 - The movement of water across a membrane is...Ch. 5 - Prob. 6UCh. 5 - Which of the following is NOT a mechanism for...Ch. 5 - A bacterial cell that can alter the composition of...Ch. 5 - What variable(s) influence(s) whether a nonpolar...Ch. 5 - Which of the following does NOT contribute to the...Ch. 5 - How are active transport and coupled transport...Ch. 5 - A cell can use the process of facilitated...Ch. 5 - figure 5.5 describes a classic experiment...Ch. 5 - Each compartment of the endomembrane system of a...Ch. 5 - The distribution of lipids in the ER membrane is...
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- A researcher is isolating proteins from a cell via different techniques. They find that when the cell is ground with a blender, all of the proteins in the cell can be extracted. However if instead the cell membranes are agitated in a 1.2 M KCl mixture, only about 70% of the total protein is extracted.What class of membrane proteins are in this soluble extract and what forces normally hold them to the membrane?What class of membrane proteins are the 30% of proteins that remain behind, and what forces hold these proteins in the membrane? If a solution of ionic solids was not available, what other solvent systems could be used to extract these proteins?arrow_forwardWhen fluorescein-dyed human proteins were first mixed with rhodamine-dyed mouse proteins (in mouse/human hybrid cell fusions carried out in the 1970s), these proteins appeared to exhibit: restricted movement, based on confinement by diffusion barriers restricted movement, based on tethering to extracellular molecules unrestricted movement, similar to membrane phospholipids restricted movement, based on anchoring to intracellular proteins restricted movement, based on attachment to other cellsarrow_forwardYou are interested in the mobility of two different unknown integral plasma membrane proteins—protein X and protein Y—in a cell. In one cell, you fluorescently label all the X proteins. In another cell, you label all the Y proteins. When you perform FRAP analysis on both cells, you find that most of the protein X fluorescence has recovered within 3 minutes, but recovery of the protein Y fluorescence is significantly slower. Even after 10 minutes, only 50% of the protein Y fluorescence has recovered. Describe the FRAP experimental protocol, explain what the results tell you about mobility of the X and Y proteins, and propose a possible explanation for why the mobilities of the two proteins differ.arrow_forward
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