Microbiology: An Introduction, Global Edition
12th Edition
ISBN: 9781292099149
Author: Tortora J. Gerard
Publisher: PEARSON
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Question
Chapter 4, Problem 7R
Summary Introduction
To review:
The cell wall components between gram-positive and gram-negative bacteria.
Introduction:
The cell wall of a bacteria is a slightly rigid structure that gives the shape of the cell. It is made up of an inner and an outer membrane which give protection to the cell from adverse conditions. The cell wall is present in most of the prokaryotes which serve as an attachment site of flagella, site to cause diseases and a site of antibiotic action.
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a. What can you observe in viewing your stained bacterial slide under the microscope if you fixed a lot of bacterial colonies in your slide during smear preparation?
b. What can you observe in viewing your stained bacterial slide under the microscope if you heat fixed your slide in a much longer exposure to heat?
Bacillis brevis are lysed using a valve-type homogenizer. The extent of disruption depends on the applied pressure and the number of passes through the homogenizer chamber and can be described by the equation, ln(1 − ?) = −????.
Is Bacillis brevis a gram-positive or gram-negative bacterial cell? Based on this response,
describe the cell wall structure and how it differs from the other type.
Develop an equation for calculation of pressure as a function of the number of passes through the homogenizer.
What factor(s) did you take into consideration when selecting the number of passes?
Escherichia coli cells are lysed with the same homogenization, is this organism gram-positive or gram-negative?
What impact does increasing the value of the constant, a, have on the required number of passes at a given pressure? What factors impact the value of a?
If fine cell debris after homogenization makes subsequent solid-liquid separation difficult, what would you recommend?
Answer as…
Answer the following questions:
1. Define a bacterial colony.
2. What is the difference between macroscopic and microscopic appearance of bacteria?
3. State the three standard terms used to describe single colonies on agar plates.
4. State and define the three types of growth that may be seen in a broth culture.
5. State three basic shapes of bacteria.
6. State and describe the different arrangements of cocci.
7. What is the difference between true motility and Brownian motion?
Chapter 4 Solutions
Microbiology: An Introduction, Global Edition
Ch. 4 - DRAW IT Diagram each of the following flagellar...Ch. 4 - Endospore formation is called (a) _____. It is...Ch. 4 - DRAW IT Draw the bacterial shapes listed in (a),...Ch. 4 - Match the structures in column A to their...Ch. 4 - Why is an endospore called a resting structure? Of...Ch. 4 - Compare and contrast the following a. simple...Ch. 4 - Prob. 7RCh. 4 - Starch is readily metabolized by many cells, but a...Ch. 4 - Match the characteristics of eukaryotic cells in...Ch. 4 - NAME IT What group of microbes is characterized by...
Ch. 4 - Which of the following is not a distinguishing...Ch. 4 - Which statement best describes what happens when a...Ch. 4 - Which statement best describes what happens when a...Ch. 4 - Which statement best describes what happens when a...Ch. 4 - Which of the following statements best describes...Ch. 4 - Which of the following is false about fimbriae? a....Ch. 4 - Which of the following pairs is mismatched? a....Ch. 4 - Which of the following pairs is mismatched? a....Ch. 4 - You have isolated a motile, gram-positive cell...Ch. 4 - The antibiotic amphothericin B disrupts plasma...Ch. 4 - How can prokaryotic cells be smaller than...Ch. 4 - The smallest eukaryotic cell is the motile alga...Ch. 4 - Two types of prokaryotic cells have been...Ch. 4 - In 1985, a 0.5-mm cell was discovered in...Ch. 4 - When E. coli cells are exposed to a hypertonic...Ch. 4 - Clostridium botulinum is a strict anaerobe; that...Ch. 4 - A South San Francisco child enjoyed bath time at...Ch. 4 - Live cultures of Bacillus thuringiensis (Dipel)...
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