Microbiology with Diseases by Body System (5th Edition)
5th Edition
ISBN: 9780134477206
Author: Robert W. Bauman Ph.D.
Publisher: PEARSON
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Chapter 4, Problem 6MC
Summary Introduction
Introduction:
Various types of lights have been used in several classes of microscopes to examine specimens. The light microscopy is classified into four types based on the application of light on the image to get its appropriate view.
In transmission electron microscopy, a beam of electrons is generated to produce the image on a fluorescent background. It is used to study the internal details of a specimen.
The light microscopy and the transmission electron microscopy differ from each other on the basis of their magnification power, resolution, and wavelength.
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FRET microscopy can be used for:
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a. Do you open both eyes or close one eye when viewing a specimen under a microscope? Why? Why not?
b. In using a compound microscope, do you start with the low power objective (LPO) or the high power objective (HPO)? Why or Why not?
c. When using 60X, do you it together with the coarse adjustment knob or fine adjustment knob?
d. Why must you slowly turn the fine adjustment knob?
e. Is natural light or is artificial light used when using the microscope?
As with standard phase contrast microscopy,differential-interference contrast microscopy depends on differences in the ___________________ of a speciment to create high-contrast images.
a. refractive index
b. light absorption
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Chapter 4 Solutions
Microbiology with Diseases by Body System (5th Edition)
Ch. 4 - Prob. 1TMWCh. 4 - Why is a Gram-negative bacterium colorless but a...Ch. 4 - Why didnt Linnaeus create taxonomic groups for...Ch. 4 - Necrotizing Fasciitis Fever, chills, nausea,...Ch. 4 - Why is magnification high and color absent in an...Ch. 4 - Prob. 1MCCh. 4 - Prob. 2MCCh. 4 - Prob. 3MCCh. 4 - Curved glass lenses _________ light. a. refract b....Ch. 4 - Prob. 5MC
Ch. 4 - Prob. 6MCCh. 4 - Prob. 7MCCh. 4 - Prob. 8MCCh. 4 - Prob. 9MCCh. 4 - In the binomial system at nomenclature, which term...Ch. 4 - Prob. 1FIBCh. 4 - Prob. 2FIBCh. 4 - Prob. 3FIBCh. 4 - Fill in the Blanks 4. ___________ refers to...Ch. 4 - Fill in the Blanks 5. Cationic chromophores such...Ch. 4 - Prob. 1VICh. 4 - Label the microscope.Ch. 4 - Prob. 1SACh. 4 - Critique the following definition of magnification...Ch. 4 - Prob. 3SACh. 4 - Put the following substances in the order they are...Ch. 4 - Prob. 5SACh. 4 - Prob. 6SACh. 4 - Prob. 7SACh. 4 - Prob. 8SACh. 4 - Miki came home from microbiology lab with green...Ch. 4 - Why is the definition of species as successfully...Ch. 4 - With the exception of the discovery of new...Ch. 4 - Prob. 4CTCh. 4 - Prob. 5CTCh. 4 - In what ways are the Gram stain and the acid-fast...Ch. 4 - Microbiologists have announced the discovery of...Ch. 4 - Why is the genus name Coccus placed within...Ch. 4 - A clinician obtains a specimen of urine from a...Ch. 4 - Using the following terms, fill in the following...
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- B. Write the function/s of each part of the microscope listed below. a. Eyepiece b. Draw tube c. Hemispheric prism housing d. Dust shield e. Revolving Nose Piece f. Objectives: Scanner LPO HPO ΟΙΟ g. Arm h. Coarse Adjustment knob i. Fine adjustment knob j. Slide holder & clip k. Rear knob I. Front knob m. Central aperture n. Condenser o. Iris diaphragm p. Mechanical Stage q. Mirror r. Mirror rack s. Stage adjustment knob t. Basearrow_forwardWhich microscopy technique is BEST at determining the presence of physical microbial interaction(s)? a Light microscopy. b Scanning or transmission electron microscopy. c Fluorescent microscopy. d Phase contrast microscopy.arrow_forwardA phase-contrast microscope differs from a brightfield microscope by having a a. blue filter in the ocular lens. b. diaphragm with an annular stop. c. phase plate in the objective lens. d. Both (b) and (c) are correct. e. All are correct.arrow_forward
- The resolving power of the microscope reveals: a. Whether or not oil is needed to see a microorganism. b. The maximum magnification possible with a particular objective. closely spaced objects to be seen clearly. c. Two closely spaced objects to be seen clearly. d. The magnification capacity of the microscope E.the amount of clearance between slide and objective lens.arrow_forwardA microscope that maintains focus when the objective magnification is increased is calleda. binocular.b. myopic.c. parfocal.d. refractive.e. resolute.arrow_forwardIn light microscopy, when magnification is increased, the lens focuses closer to the sample. This makes the user more likely to ram the sample into the lens. Why is it common for the lens to focus closer to the sample at higher magnifications? What can be done to mitigate this risk?arrow_forward
- What is/are the characteristics of an ideal microscope? I. Low f-number. II. High f-number. III. Short wavelength of "light" used IV. Long wavelength of "light" used A. I only. B. II only. C. III only. D. Both I and III. E. Both II and IV.arrow_forwardWhat part of the microscope adjusts the magnification of the microscope? Group of answer choices A. objective lens B. on/off switch C. iris D. course adjustmentarrow_forwardWhat part of the microscope adjusts the amount of light projected onto the slide? Group of answer choices A. rheostat B. on/off switch C. iris D. course adjustmentarrow_forward
- Which of the following statements are true in the context of the microscope's field of view? I. It is the observable area within a circular space as defined by the lenses II. FOV is the smallest on the lowest power objective III. FOV is the maximum area visible through the microscope eyepiece IV. When you switch to a higher power, the field of zooms out towards the centerarrow_forwardParagraph 3. If the ocular lens on a microscope has a magnification of 20OX, what is the total magnification of: a. a scanning lens with magnification of 4X b. a low-power lens with a magnification of 1oX c. a high power lens with a magnification of 40X d. an oil-immersion lens with a magnification of 100X 4. Choose how the letter "R" will appear as viewed under a microscope. R B R As viewed with the naked eye b. a. P. 5. True/False a. Begin the focusing process with the high-power objective. b. Store the microscope with the oil-immersion objective in position. c. Use tissue d. The coarse adjustment knob can be used with any objective lens. раper and water to clean the ocular and objective lenses.arrow_forwardDescribe the ff types of microscope: A. Darkfield microscope B. Phase contrast microscope C. Differential interfetence microscopearrow_forward
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