Microbiology with Diseases by Taxonomy (5th Edition)
5th Edition
ISBN: 9780134019192
Author: Robert W. Bauman Ph.D.
Publisher: PEARSON
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Chapter 4, Problem 4SA
Summary Introduction
To identify:
The order of substance used in
Introduction:
Differential stains are mostly used in
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Which of the following serves as a counterstain for the gram-stain technique? It also happens to be the counterstain for the endospore staining technique.
What is the purpose of each reagent in gram-staining?
Primary stain
Mordant
Decolorizing agent
Counterstain
List the primary stain, secondary stain, mordant, and decolorizer for the gram stain,
and write them in the order that you would use them. Make sure to label which is
which.
Chapter 4 Solutions
Microbiology with Diseases by Taxonomy (5th Edition)
Ch. 4 - Prob. 1MCCh. 4 - A nanometer is ________ than a micrometer. a. 10...Ch. 4 - Prob. 3MCCh. 4 - Curved glass lenses _________ light. a. refract b....Ch. 4 - Prob. 5MCCh. 4 - Prob. 6MCCh. 4 - Which of the following types of microscopes...Ch. 4 - Prob. 8MCCh. 4 - Prob. 9MCCh. 4 - Prob. 10MC
Ch. 4 - If an objective magnifies 40 and each binocular...Ch. 4 - The type of fixation developed by Koch for...Ch. 4 - Immersion oil ________ (increases/decreases) the...Ch. 4 - Prob. 4FIBCh. 4 - Prob. 5FIBCh. 4 - Prob. 1VICh. 4 - Label the microscope.Ch. 4 - Prob. 1SACh. 4 - Critique the following definition of magnification...Ch. 4 - Prob. 3SACh. 4 - Prob. 4SACh. 4 - Prob. 5SACh. 4 - Give three characteristics of a specific epithet.Ch. 4 - Prob. 7SACh. 4 - Prob. 8SACh. 4 - Prob. 1CTCh. 4 - Prob. 2CTCh. 4 - Prob. 3CTCh. 4 - Prob. 4CTCh. 4 - Prob. 5CTCh. 4 - In what ways are the Gram stain and the acid-fast...Ch. 4 - Microbiologists have announced the discovery of...Ch. 4 - Why is the genus name Coccus placed within...Ch. 4 - A clinician obtains a specimen of urine from a...Ch. 4 - Using the following terms, fill in the following...Ch. 4 - Prob. 1TMWCh. 4 - Prob. 2TMWCh. 4 - Prob. 3TMWCh. 4 - Prob. 4TMWCh. 4 - Prob. 1EDCS
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- Which of the following are true about a differential stain. There may be more than one answer! No chemicals are needed. More than one stain is used The acid fast stain is an example The Gram stain is an example.arrow_forwardList the reagents of the Gram stain technique in order and their general role in the staining process.arrow_forwardCreate paragraphs to differentiate among the following terms: positive stain, negative stain, simple stain, and differential stain. please provide minimum of 400 wordsarrow_forward
- If you Gram stain a bacterium that is gram-negative, which dye will be visible once the stain is completed? Crystal violet Safranin Decolorizer lodinearrow_forwardName all the reagents used in Gram-staining and their roles in the staining procedure.arrow_forwardYou are performing a Gram stain on Gram-negative bacteria, and you stop after the addition of the alcohol. What is the appearance of the bacteria at this point? ○ pink ○ colorless ○ blue ○ purplearrow_forward
- Draw what you would expect to see for acid fast stain and endospore stain procedure if you did not stain with counterstain. Color pencils need to be used.arrow_forwardList the steps of the gram staining procedure in proper sequence and indicate the color of gram-positive and gram-negative bacteria after each step.arrow_forwardFill in the blank: The gram stain is a ______________ stain in which a decolorization step occurs between the application of two basic stains.arrow_forward
- This part of the staining process helps the primary stain to remain in the cell during decolorization. O 1) Secondary stain O 2) primary stain O 3) counterstain O 4) fixing reagent O 5) mordantarrow_forwardThe acid-fast stain is used to stain _____. The decolorizer used to destain the cells is ______. Positive cells that retain the primary stain are ______, while negative cells are counterstained with _____ and will have a ______ color. *Please use answers from the options provided in the image below.arrow_forwardThe first step in identifying an organism from a pure working culture is to perform a(n) __________ test. acid-fast staining negative staining endospore staining simple staining Gram stainarrow_forward
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