Prescott's Microbiology
11th Edition
ISBN: 9781260211887
Author: WILLEY, Sandman, Wood
Publisher: McGraw Hill
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Textbook Question
Chapter 37, Problem 1AL
ELISA tests usually use a primary and secondary antibody. Why? What are the necessary controls one would need to perform to ensure that the antibody specificities are valid (i.e., no false-positive or false-negative reactions)?
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Chapter 37 Solutions
Prescott's Microbiology
Ch. 37.2 - Prob. 1MICh. 37.2 - Prob. 2MICh. 37.2 - Prob. 1CCCh. 37.2 - Describe in general how biochemical tests are used...Ch. 37.2 - Prob. 3CCCh. 37.2 - Prob. 4CCCh. 37.2 - Prob. 5CCCh. 37.2 - Prob. 6CCCh. 37.3 - Prob. 1MICh. 37.3 - Prob. 2MI
Ch. 37.3 - Prob. 1CCCh. 37.3 - Prob. 2CCCh. 37.3 - Prob. 3CCCh. 37.3 - Prob. 4CCCh. 37.3 - Prob. 5CCCh. 37.3 - Name two types of immunodiffusion tests and...Ch. 37 - Prob. 1RCCh. 37 - Prob. 2RCCh. 37 - Prob. 3RCCh. 37 - Prob. 4RCCh. 37 - ELISA tests usually use a primary and secondary...Ch. 37 - Prob. 2ALCh. 37 - Prob. 3AL
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- What is the effect of not adding the primary antibody to the ELISA reaction?arrow_forwardWhat is the difference between an indirect ELISA and a sandwich ELISA? Is rapid antibody testing a form of ELISA?arrow_forwardWhat are the advantages and disadvantages of agglutinationtests versus fluorescent antibody assays? How are the latter usedto identify specific cells in complex mixtures, such as blood?arrow_forward
- What is the result of the Antibody Screening Test? Based on the result of the autocontrol, it is safe to say that the antibodies which reacted with the screening cells and panel cells are?arrow_forwardIf you were using the ELISA to look for the presence of antibodies and the sample gave a negative result, does this mean that there were no antibodies present? Explain.arrow_forwardWhy do some tests for antibody in serum (such as for HIV and syphilis)require backup verification with additional tests at a later date?arrow_forward
- To diagnosis, we used a procedure known as a "sandwich ELISA" (Enzyme-Linked Immunosorbent Assay). 1.In the "ELISA sandwich," what is the "meat"? 2.Both patients A and B had positive results. Why are these two patients' outcomes so different? 3.What is one potential cause of an ELISA false negative result? What is one potential cause of an ELISA false positive result? What is another example of how an ELISA assay should be used?arrow_forwardConjugated enzymes are a key component of ELISA. To what are these enzymes conjugated, and why is that important? To the antigen so the enzymes are only present when the molecule of interest is also present To the plastic of the reaction well so the enzymes can concentrate the molecule of interest To the capture antibody so the enzymes are only present when the molecule of interest is also present To the detection antibody so the enzymes are only present when the molecule of interest is also presentarrow_forwardSuppose you were instructed to make two antiserum solutions: The first is identical to what you used in the lab exercise. The other is a 10:6 dilution of the antiserum. After incubation with the antigen, the full-strength antiserum produces a precipitous ring, but the diluted antiserum does not. Explain these results. Is this due to poor specificity or sensitivity of the test?arrow_forward
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