To review:
A genetic screening to identify the phyC –deficient mutants and the type of
Given:
It is given in the experiment that the plants in which the gene for phy C was overexpressed shows an increase in the leaf area relative to the wild type.
Table: Phtochromes phyA, phyB, phyC, and phyD were separately overexpressed in Arabidopsis, and 18-day-old seedlings were examined with regard to leaf area.
Gene expression | Leaf area (mm2) |
Wild-type | 22 |
phyA overexpressed | 21 |
phyB overexpressed | 21 |
phyC overexpressed | 31 |
phyD overexpressed | 22 |
Introduction:
The genetic screen is an important technique that can be used to identify certain genes involved in a particular function. It is an experimental technique in which individuals that possess a certain type of phenotype are identified from a mutagenized population. Genetic screens are helpful in studying functions of genes of interest.
Want to see the full answer?
Check out a sample textbook solutionChapter 36 Solutions
Life: The Science of Biology
- How do you design a reporter assay for Serpina1?arrow_forwardWhat detection method could be used to determine the Mutation at a palindromic site and what results could be achieved from that?arrow_forwardCan you suggest how we could have determined whether Bill’s aunts were carriers of the mutant BTK gene? Give the name for the procedure and brief explanation of how it’s done and the anticipated results. What controls would be needed?arrow_forward
- How would you modify the Ames test to evaluate physical mutagens?Would it be necessary to add the rat liver extract? Explain why or why not.arrow_forwardYou can carry out matings between an Hfr and F strain by mixing the two cell types in a small patch on a plate and then replica plating to selective medium. This methodology was used to screen hundreds of different cells for a recombination-deficient recA - mutant. Why is this an assay for RecA function? Would you be screening for a recA mutation in the F or Hfr strain using this protocol? Explain.arrow_forwardWhat are site-recombinases? Describe in detail how cre- recombinase can be used to decipher the roles of specific genes and proteins in complex multicellular organisms? Explain how the cre-lox system can be used to examine the role of a particular gene in a specific type of tissue?arrow_forward
- How can we use the transposon sequence to find where the mutation occurred in a forward genetic screen.arrow_forwardBriefly describe the method of chromatin immunoprecipitation sequencing (ChIP-Seq). How is it used to determine nucleosome positions within a genome?arrow_forwardWhat detection method could be used to determine the mutagen added to a microbial culture resulting in an altered gene A of the organism and what results could be achieved from that?arrow_forward
- Outline a PCR-based approach to screen for colonies transformed with recombinant expression plasmid that can be used for protein expression.arrow_forwardAfter mutagenesis of wild type Vibrio fisheri, you isolate two different mutant strains (A and B) that, unlike the wild type cells, fail to luminesce when grown to high density in a flask with appropriate medium. Curiously, however, when you inoculate both mutant strains in the same flask, you observe that the mixed (A+B) culture begins to emit light after growing dense. a) What gene/functions are likely affected in each of the two mutants? b) How does this explain their phenotypes?arrow_forwardExplain how site directed mutagenesis can be accomplished using M13 bacteriophage. Using this method often results in only 5% of plaques with mutant gene. Explain how the procedure has been modified to enrich for the number of plaques with the mutant gene.arrow_forward
- Case Studies In Health Information ManagementBiologyISBN:9781337676908Author:SCHNERINGPublisher:Cengage