Prescott's Microbiology
Prescott's Microbiology
11th Edition
ISBN: 9781260211887
Author: WILLEY, Sandman, Wood
Publisher: McGraw Hill
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Chapter 34, Problem 3RC
Summary Introduction

To determine: The identification of microbes by using small subunit ribosomal RNA.

Introduction: There are various types of approaches used for classification and identification of different micro-organisms, which have been isolated and cultured. The two types of approaches are: classical and molecular

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1) Given an mRNA with the following sequence, please translate the codons to a chain of amino acids. Use the codon chart below.5’AUG/CCU/GCU/UAC/CGG/GAG/UAA3’ “met-_________-_________-_________-_________-_________”STOP”   2) Assuming the original polypeptide chain below, match each type of point mutation with the polypeptide chain that results.   Original polypeptide: Pro-Thr-Ser-Leu-Leu-His-Asn A.    Missense B.    Silent C.     Nonsense D.    Frameshift   ______ Pro-Thr-Ser-STOP     ______ Pro-Thr-Ser-Leu-Ile-His-Asn     ______ Pro-Thr-Ser-Leu-Leu-His-Asn     _______ Pro-Thr-His-Cys-Tyr-Thr
Referring to the Standard Genetic Code table, categorize the chemical properties of each of the 24 amino acids that make up the ER Signal Peptide (hydrophobic, hydrophilic, positive charge, or negative charge). What is notable about the chemical properties of the amino acids that make up the ER Signal Peptide? methionine- translation code tra-hyperopic amino acid R Origine eukiauk aicd amino  glutamine HYDOICO ACIDS  gaac -CHANGED AMINO ACID
1) Given an mRNA with the following sequence, please translate the codons to a chain of amino acids. Use the codon chart below.5’AUG/CCU/GCU/UAC/CGG/GAG/UAA3’ “met-_________-_________-_________-_________-_________”STOP”   2) Assuming the original polypeptide chain below, match each type of point mutation with the polypeptide chain that results.   Original polypeptide: Pro-Thr-Ser-Leu-Leu-His-Asn A.    Missense B.    Silent C.     Nonsense D.    Frameshift   ______ Pro-Thr-Ser-STOP     ______ Pro-Thr-Ser-Leu-Ile-His-Asn     ______ Pro-Thr-Ser-Leu-Leu-His-Asn     _______ Pro-Thr-His-Cys-Tyr-Thr
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