Pearson eText Microbiology: An Introduction -- Instant Access (Pearson+)
13th Edition
ISBN: 9780135789377
Author: Gerard Tortora, Berdell Funke
Publisher: PEARSON+
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Chapter 3, Problem 4A
Endospores can be seen as refractile structures in unstained cells and as colorless areas in
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Endospores can be seen as refractile structures in unstained cells and as colorless areas in Gram-stained cells. Why is it necessary to do an endospore stain to verify the presence of endospores?
1) In Gram staining, to what cell structure do the dyes bind?
2) Would it be useful to perform a Gram stain on a mixed culture? Why?
3) In capsule staining, why does the capsules did NOT take in any dye?
4) In endospore staining, what is the purpose of using the steam?
Additional Differential Staining Procedures: Negative stain, Capsule Stain, Endospore stain, Flagella stain. What is a positive and negative results of this procedures?
Chapter 3 Solutions
Pearson eText Microbiology: An Introduction -- Instant Access (Pearson+)
Ch. 3 - Fill in the following blanks. 1. 1 m = ________ m...Ch. 3 - Prob. 2RCh. 3 - Prob. 3RCh. 3 - Prob. 4RCh. 3 - Prob. 5RCh. 3 - Why is a mordant used in the Gram stain? In the...Ch. 3 - Prob. 7RCh. 3 - Prob. 8RCh. 3 - Fill in the following table regarding the Gram...Ch. 3 - NAME IT A sputum sample from Calle, a 30-year-old...
Ch. 3 - Through the microscope, the green structures are...Ch. 3 - Prob. 2MCQCh. 3 - Carbolfuchsin can be used as a simple stain and a...Ch. 3 - Prob. 4MCQCh. 3 - Which of the following is not a functionally...Ch. 3 - Which of the following pairs is mismatched? 1....Ch. 3 - Assume you stain Clostridium by applying a basic...Ch. 3 - Prob. 8MCQCh. 3 - In 1996, scientists described a new tapeworm...Ch. 3 - Prob. 10MCQCh. 3 - Prob. 1ACh. 3 - Prob. 2ACh. 3 - Why isnt the Gram stain used on acid-fast...Ch. 3 - Endospores can be seen as refractile structures in...Ch. 3 - In 1882, German bacteriologist Paul Erhlich...Ch. 3 - Laboratory diagnosis of Neisseria gonorrhoeae...Ch. 3 - Assume that you are viewing a Gram-stained sample...
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- What are the structural differences between Gram-positive and Gram-negative bacteria cell walls? Can you explain what would happen to the cell wall's physical integrity while applying the Gram staining technique?arrow_forwardWhat benefits do endospores provide the cells during staining procedures?arrow_forwardWhy do you suppose endospore-forming bacteria are commonly found in the soil? Suppose your unknown bacteria is a Gram-negative rod. You decide to perform an endospore stain, thinking it will help you with the identification of the organism. What will you see, and why?arrow_forward
- Are there any chemical differences between the cell walls of gram-positive and negative bacteria that might explain differences in the rate of decolorization ? Does the age of a culture affect the gram stain reaction ? Why or why not?arrow_forwardThe bacterial cells in the image below were stained with a high concentration of carbolfuschin for an extended period of time (5 minutes) while the slide was heated. The slide was decolorized with a mixture of acid and alcohol. The slide was then counter-stained with methylene blue. What kind of stain does this describe?arrow_forwardwhy is it necessary to employ a spore stain rather than rely on diagnosis of sporing from examination of gram stainarrow_forward
- An acid-fast stain can be used to detect protective coverings found in using light microscopy. organisms incorporating mycolic acid in the cell wall organisms forming endospores organisms with capsules organisms with Gram-negative cell wallsarrow_forwardWhich of the following serves as a counterstain for the gram-stain technique? It also happens to be the counterstain for the endospore staining technique.arrow_forwardWhat staining technique is used here? What is the primary stain, decolorizing agent, and counter stain of this technique? Do we heat fix in here? Describe the organism with the blue color. Describe the organism with the bright pink color. Name the genus of bacteria with bright pink.arrow_forward
- What is the purpose of iodine in the gram staining process? Explain.arrow_forwardwhat color are endospores at the conclusion of the endospore stain?arrow_forwardWhy does endospore staining require heating? What chemical component of the endosporesprotects the cell from high temperature and chemical treatment?arrow_forward
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