MICROBIOLOGY W/ACCESS
4th Edition
ISBN: 9781266808685
Author: Cowan
Publisher: MCG
expand_more
expand_more
format_list_bulleted
Concept explainers
Videos
Textbook Question
Chapter 3, Problem 1VC
Visual Connections Bloom’s Level 5: Evaluate
These questions use visual images or previous content to make connections to this chapter’s concepts.
1. Process Figure 3.10. If you were using the streak plate method to plate a very dilute broth culture (with many fewer bacteria than the broth used here) would you expect to see single, isolated colonies in area 4 or area 3? Explain your answer.
Note: This method only works if the spreading tool (using an inoculating loop) is resterilized after each of steps 1-4.
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
Examine figure a, b (shown here). If you performed the quadrantstreak plate method using a broth culture that had 10× fewer cellsthan the broth used in the culture shown, which quadrant might youexpect to yield isolated colonies? Explain your answer.
Data
Both plates were inoculated with the same 4 bacteria and in the same pattern, but
were incubated in either the CampyPak or GasPak systems. Compare the growth
on either plate and log the data in the table in the next slide.
Answer the questions on the next slide.
GasPak Plus Pouch for growth
under anaerobic conditions
CampyPak Pouch for growth under
microaerophilic conditions (similar
to candle jar technique)
S. PYOGENES
S. PYOGENES
M. LUTEUS
M. LUTEUS
C. SPOROGENES
E. COLI
C. SPOROGENES
Step #1 Culture and perform a Gram-stain on the
Super Bug
You culture the Super Bug by swabbing the patient's wound,
applying the sample to the surface of an agar plate (petri dish),
and incubating the plate for one day. Next you perform a Gram
stain on a pure culture of the Super Bug cells.
1. Considering the bacteria were originally isolated from an
exposed wound on a human patient, under what conditions
(temperature, atmosphere) did you most likely incubate your
plate of Super Bug cells to achieve maximal growth?
Gram stain observations: Short rod-shaped cells found in
clusters, stained red/pink
2. Is the Super Bug Gram negative or positive?
3. What conclusions can you make about the components of the
Super Bug cell wall?
Chapter 3 Solutions
MICROBIOLOGY W/ACCESS
Ch. 3.1 - Prob. 2CFCh. 3.1 - Explain what the Five Is mean and what each step...Ch. 3.1 - Discuss three physical states of media and when...Ch. 3.1 - Compare and contrast selective and differential...Ch. 3.1 - Provide brief definitions for defined and complex...Ch. 3.2 - Prob. 5AYPCh. 3.2 - Prob. 6AYPCh. 3.2 - Prob. 7AYPCh. 3.2 - Prob. 8AYPCh. 3.2 - Compare and contrast the three main categories of...
Ch. 3 - Prob. 1CFCh. 3 - Prob. 1MCQCh. 3 - A mixed culture is a. the same as a contaminated...Ch. 3 - Resolution is ____ with a longer wavelength of...Ch. 3 - A real image is produced by the a. ocular. b....Ch. 3 - Prob. 5MCQCh. 3 - The specimen for an electron microscope is always...Ch. 3 - Prob. 7MCQCh. 3 - Bacteria tend to stain more readily with cationic...Ch. 3 - Multiple Matching. For each type of medium, select...Ch. 3 - A fastidious organism must be grown on what type...Ch. 3 - Agar has the disadvantage of being easily...Ch. 3 - A subculture is a culture made from an isolated...Ch. 3 - Prob. 13TFCh. 3 - Prob. 14TFCh. 3 - The best stain to use to visualize a microorganism...Ch. 3 - Prob. 1CTQCh. 3 - Prob. 2CTQCh. 3 - Prob. 3CTQCh. 3 - What is the functional type of mannitol salt agar...Ch. 3 - Create a short paragraph to differentiate among...Ch. 3 - Prob. 6CTQCh. 3 - Prob. 7CTQCh. 3 - a.Create a paragraph to differentiate among the...Ch. 3 - Prob. 9CTQCh. 3 - You are a scientist studying a marsh area...Ch. 3 - Prob. 1CCCh. 3 - Prob. 2CCCh. 3 - Prob. 3CCCh. 3 - Prob. 4CCCh. 3 - Prob. 5CCCh. 3 - Visual Connections Blooms Level 5: Evaluate These...Ch. 3 - Prob. 1CM
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Show steps plsarrow_forwardPlease answer fast Dilution Problem. A culture of Staphylococcus is diluted as follows: (1) 20mL are added to 80mL of water. (2) 10uL from (1) are then added to 9.99mL of water. (3) A 10-2 dilution is made from tube # (2). (4) 100uL from (3) are plated for a pour plate and incubated. Growth Problem.A culture with approximately 2x103 cells/mL were incubated. After 3 hours, the number of cells had increased to 3x105. a) How long was the generation time in minutes?b) How many generations have occurredarrow_forwardEXPERIMENT:EFFECT OF CARBON SOURCES ON THE MICROBIAL GROWTH. 1.OBJECTIVE To study the effect of media components on microbial growth 2.PROCEDURES Preparation of fungal suspension inoculums Prepare the laminar cabinet for the inoculum preparation by applying normal aseptic techniques To a full-grown fungal Petri dish, add 25ml sterilized distilled water and rub the agar gently with glass rod Pour the suspension into an empty flask and continue previous steps to achieve your desired volume of inoculums suspension. Cotton-plugged and aluminium-wrapped the mouth of inoculum flask. For certain fungal species like Niger, the suspension from the agar should be filtered upon pouring into the inoculum’s flask. Preparation of salt media of different carbon sources. Prepare the Modified Czapek Dox media by weighing the following salts according to your desired working volume. KH2PO4 (1 g/L) NaNO3 (3 g/L) MgSO4.7H2O (0.5 g/L) Carbon source (30 g/L) KCl (0.5 g/L) 2.…arrow_forward
- can you please do #1arrow_forwardDichotomous key: d. Since gram-negative bacteria are generally more resistant to antibiotics such as penicillin, Gram staining can be used to inform appropriate antibiotic treatment for patients with bacterial infections. Based on the information in the key, infections caused by bacteria belonging to which genera would be most effectively treated with penicillin?arrow_forwardPlease match the type of culture to the statement that most accurately describes it to test your understanding of the different types of cultures. please look at attached images.arrow_forward
- Please asaparrow_forwardIs the Tryptoc Soy Agar (TSA) a complex defined medium? Explaining based on its composition? Is TSA a differential, selective, both differential and selective media? Please help by explaining based on what kind of MicroOrganisms it allows to grow?arrow_forwardPlease show your work(calculations)arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)BiologyISBN:9780134580999Author:Elaine N. Marieb, Katja N. HoehnPublisher:PEARSON
Biology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStax
Anatomy & PhysiologyBiologyISBN:9781259398629Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa StouterPublisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)BiologyISBN:9780815344322Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter WalterPublisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & PhysiologyBiologyISBN:9781260159363Author:Martin, Terry R., Prentice-craver, CynthiaPublisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)BiologyISBN:9781260231700Author:Sylvia S. Mader, Michael WindelspechtPublisher:McGraw Hill Education
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:PEARSON
Biology 2e
Biology
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:OpenStax
Anatomy & Physiology
Biology
ISBN:9781259398629
Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:9780815344322
Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:9781260159363
Author:Martin, Terry R., Prentice-craver, Cynthia
Publisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:9781260231700
Author:Sylvia S. Mader, Michael Windelspecht
Publisher:McGraw Hill Education
Nitrogen emissions: environmental and health hazards; Author: Sandec Eawag;https://www.youtube.com/watch?v=iYcchHZ5Ejo;License: Standard Youtube License