Biochemistry (Looseleaf)
Biochemistry (Looseleaf)
9th Edition
ISBN: 9781319114800
Author: BERG
Publisher: MAC HIGHER
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Chapter 3, Problem 17P
Interpretation Introduction

Interpretation:

The name and reason for two indistinguishable amino acids in the peptide sequencing by the tandem mass spectroscopy technique should be determined.

Concept introduction:

A peptide bond exists between the two amino acids. During the formation of a peptide bond, a molecule of water is released. The carboxyl group of one amino acid gets linked with the amino group of the other amino acid. Polypeptides and proteins are the chains formed by the amino acids.

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Please ASAP. Thank you. Regarding the double helix of DNA, which of the following is true?   a. Guanine pairs up with cytosine with three hydrogen bonds   b. Complementary strands of DNA are held together by covalent bonds   c. The backbone consists of ribose sugars H-bonded to phosphate groups   d. Uracil pairs up with adenine with two hydrogen bonds
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Peptide mass determination. You have isolated a proteinfrom the bacterium E. coli and seek to confirm its identityby trypsin digestion and mass spectrometry. Determinationof the masses of several peptide fragments has enabled youto deduce the identity of the protein. However, there is adiscrepancy with one of the peptide fragments, whichyou believe should have the sequence MLNSFK and an(M 1 H)1 value of 739.38. In your experiments, yourepeat edly obtain an (M 1 H)1 value of 767.38. What isthe cause of this discrepancy and what does it tell youabout the region of the protein from which this peptide isderived?
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