The activated intermediate present in the linkage of the enzymes, DNA polymerase I, DNA ligase and topoisomerase I are to be stated. The leaving groups present in the enzymes are to be stated. Concept introduction: DNA stands for deoxyribonucleic acid, is a biological macromolecule. DNA contains double helical strands along with the complementary base pairs. The four complementary bases of DNA are adenine (A), thymine (T), guanine (G) and cytosine (C). In the formation of recombinant DNA, the restriction enzymes are involved to cut the particular region in the DNA molecule.

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Answer 1

Question

Chapter 29, Problem 1P

Interpretation Introduction

Interpretation:

The activated intermediate present in the linkage of the enzymes, DNA polymerase I, DNA ligase and topoisomerase I are to be stated. The leaving groups present in the enzymes are to be stated.

Concept introduction:

DNA stands for deoxyribonucleic acid, is a biological macromolecule. DNA contains double helical strands along with the complementary base pairs. The four complementary bases of DNA are adenine (A), thymine (T), guanine (G) and cytosine (C). In the formation of recombinant DNA, the restriction enzymes are involved to cut the particular region in the DNA molecule.

Expert Solution & Answer

Answer to Problem 1P

The activating intermediate present in the linkage of the enzymes, DNA polymerase I, DNA ligase and topoisomerase I are deoxynucleotide triphosphate, DNA adenylate and DNA-tyrosyl intermediate respectively. The leaving groups present in the enzymes, DNA polymerase I, DNA ligase and topoisomerase I are pyrophosphate, AMP and DNA- tyrosine residue respectively.

Explanation of Solution

It is given that the enzymes, DNA polymerase I, DNA ligase and topoisomerase I help in catalyzing the formation of phosphodiaster bonds.

The activated intermediate present in case of DNA polymerase I is deoxynucleotide triphosphate and the leaving group present in this enzyme is pyrophosphate.

The activated intermediate present in case of DNA ligase is DNA adenylate, in which AMP is attached to 5' phosphate and the leaving group present in this enzyme is adenosine monophosphate(AMP).

The activated intermediate present in case of topoisomerase I is DNA-tyrosyl intermediate, in which 5' phosphate is attached to phenolic −OH group and the leaving group present in this enzyme is the tyrosine residue.

Conclusion

The activating intermediate present in the linkage of the given enzymes are deoxynucleotide triphosphate, DNA adenylate and DNA-tyrosyl intermediate respectively. The leaving groups present in the enzymes are pyrophosphate, AMP and DNA- tyrosine residue respectively.

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Students have asked these similar questions

Polymerase inhibition. Cordycepin inhibits poly(A) synthesis at low concentrations and RNA synthesis at higher concentrations. NH2 H. он Cordycepin (3'-deoxyadenosine) a. What is the basis of inhibition by cordycepin? b. Why is poly(A) synthesis more sensitive than the synthesis of other RNAS to the presence of cordycepin? c. Does cordycepin need to be modified to exert its effect?

True or False. Topoisomerase I does not require ATP to break and rejoin DNA strands because the energy of the phospho-diester bond is stored transiently in a phospho-tyrosine linkage in the enzyme’s active site. Explain your answer in 2-3 sentences.

An extra piece. In one type of mutation leading to a form of thalassemia, the mutation of a single base (G to A) generates a new 3' 3' splice site (blue in the illustration below) akin to the normal one (yellow) but farther upstream. Normal 3' end of intron 5' CCTATTGGTCTATTITCCACCCITAGGCTGCTG 3' 5' CCTATTAGTCTAIIIICCACCCTTAGGCTGCTG 3' What is the amino acid sequence of the extra segment of protein synthesized in a thalassemic patient having a mutation leading to aberrant splicing? The reading frame after the splice site begins with TCT.

Answer 2

Question

Chapter 29, Problem 1P

Interpretation Introduction

Interpretation:

The activated intermediate present in the linkage of the enzymes, DNA polymerase I, DNA ligase and topoisomerase I are to be stated. The leaving groups present in the enzymes are to be stated.

Concept introduction:

DNA stands for deoxyribonucleic acid, is a biological macromolecule. DNA contains double helical strands along with the complementary base pairs. The four complementary bases of DNA are adenine (A), thymine (T), guanine (G) and cytosine (C). In the formation of recombinant DNA, the restriction enzymes are involved to cut the particular region in the DNA molecule.

Expert Solution & Answer

Answer to Problem 1P

The activating intermediate present in the linkage of the enzymes, DNA polymerase I, DNA ligase and topoisomerase I are deoxynucleotide triphosphate, DNA adenylate and DNA-tyrosyl intermediate respectively. The leaving groups present in the enzymes, DNA polymerase I, DNA ligase and topoisomerase I are pyrophosphate, AMP and DNA- tyrosine residue respectively.

Explanation of Solution

It is given that the enzymes, DNA polymerase I, DNA ligase and topoisomerase I help in catalyzing the formation of phosphodiaster bonds.

The activated intermediate present in case of DNA polymerase I is deoxynucleotide triphosphate and the leaving group present in this enzyme is pyrophosphate.

The activated intermediate present in case of DNA ligase is DNA adenylate, in which AMP is attached to 5' phosphate and the leaving group present in this enzyme is adenosine monophosphate(AMP).

The activated intermediate present in case of topoisomerase I is DNA-tyrosyl intermediate, in which 5' phosphate is attached to phenolic −OH group and the leaving group present in this enzyme is the tyrosine residue.

Conclusion

The activating intermediate present in the linkage of the given enzymes are deoxynucleotide triphosphate, DNA adenylate and DNA-tyrosyl intermediate respectively. The leaving groups present in the enzymes are pyrophosphate, AMP and DNA- tyrosine residue respectively.

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Answer 3

Question

Chapter 29, Problem 1P

Interpretation Introduction

Interpretation:

The activated intermediate present in the linkage of the enzymes, DNA polymerase I, DNA ligase and topoisomerase I are to be stated. The leaving groups present in the enzymes are to be stated.

Concept introduction:

DNA stands for deoxyribonucleic acid, is a biological macromolecule. DNA contains double helical strands along with the complementary base pairs. The four complementary bases of DNA are adenine (A), thymine (T), guanine (G) and cytosine (C). In the formation of recombinant DNA, the restriction enzymes are involved to cut the particular region in the DNA molecule.

Expert Solution & Answer

Answer to Problem 1P

The activating intermediate present in the linkage of the enzymes, DNA polymerase I, DNA ligase and topoisomerase I are deoxynucleotide triphosphate, DNA adenylate and DNA-tyrosyl intermediate respectively. The leaving groups present in the enzymes, DNA polymerase I, DNA ligase and topoisomerase I are pyrophosphate, AMP and DNA- tyrosine residue respectively.

Explanation of Solution

It is given that the enzymes, DNA polymerase I, DNA ligase and topoisomerase I help in catalyzing the formation of phosphodiaster bonds.

The activated intermediate present in case of DNA polymerase I is deoxynucleotide triphosphate and the leaving group present in this enzyme is pyrophosphate.

The activated intermediate present in case of DNA ligase is DNA adenylate, in which AMP is attached to 5' phosphate and the leaving group present in this enzyme is adenosine monophosphate(AMP).

The activated intermediate present in case of topoisomerase I is DNA-tyrosyl intermediate, in which 5' phosphate is attached to phenolic −OH group and the leaving group present in this enzyme is the tyrosine residue.

Conclusion

The activating intermediate present in the linkage of the given enzymes are deoxynucleotide triphosphate, DNA adenylate and DNA-tyrosyl intermediate respectively. The leaving groups present in the enzymes are pyrophosphate, AMP and DNA- tyrosine residue respectively.

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Answer 4

Question

Chapter 29, Problem 1P

Interpretation Introduction

Interpretation:

The activated intermediate present in the linkage of the enzymes, DNA polymerase I, DNA ligase and topoisomerase I are to be stated. The leaving groups present in the enzymes are to be stated.

Concept introduction:

DNA stands for deoxyribonucleic acid, is a biological macromolecule. DNA contains double helical strands along with the complementary base pairs. The four complementary bases of DNA are adenine (A), thymine (T), guanine (G) and cytosine (C). In the formation of recombinant DNA, the restriction enzymes are involved to cut the particular region in the DNA molecule.

Expert Solution & Answer

Answer to Problem 1P

The activating intermediate present in the linkage of the enzymes, DNA polymerase I, DNA ligase and topoisomerase I are deoxynucleotide triphosphate, DNA adenylate and DNA-tyrosyl intermediate respectively. The leaving groups present in the enzymes, DNA polymerase I, DNA ligase and topoisomerase I are pyrophosphate, AMP and DNA- tyrosine residue respectively.

Explanation of Solution

It is given that the enzymes, DNA polymerase I, DNA ligase and topoisomerase I help in catalyzing the formation of phosphodiaster bonds.

The activated intermediate present in case of DNA polymerase I is deoxynucleotide triphosphate and the leaving group present in this enzyme is pyrophosphate.

The activated intermediate present in case of DNA ligase is DNA adenylate, in which AMP is attached to 5' phosphate and the leaving group present in this enzyme is adenosine monophosphate(AMP).

The activated intermediate present in case of topoisomerase I is DNA-tyrosyl intermediate, in which 5' phosphate is attached to phenolic −OH group and the leaving group present in this enzyme is the tyrosine residue.

Conclusion

The activating intermediate present in the linkage of the given enzymes are deoxynucleotide triphosphate, DNA adenylate and DNA-tyrosyl intermediate respectively. The leaving groups present in the enzymes are pyrophosphate, AMP and DNA- tyrosine residue respectively.

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Answer 5

Chapter 29, Problem 1P

Interpretation Introduction

Interpretation:

The activated intermediate present in the linkage of the enzymes, DNA polymerase I, DNA ligase and topoisomerase I are to be stated. The leaving groups present in the enzymes are to be stated.

Concept introduction:

DNA stands for deoxyribonucleic acid, is a biological macromolecule. DNA contains double helical strands along with the complementary base pairs. The four complementary bases of DNA are adenine (A), thymine (T), guanine (G) and cytosine (C). In the formation of recombinant DNA, the restriction enzymes are involved to cut the particular region in the DNA molecule.

Expert Solution & Answer

Answer to Problem 1P

The activating intermediate present in the linkage of the enzymes, DNA polymerase I, DNA ligase and topoisomerase I are deoxynucleotide triphosphate, DNA adenylate and DNA-tyrosyl intermediate respectively. The leaving groups present in the enzymes, DNA polymerase I, DNA ligase and topoisomerase I are pyrophosphate, AMP and DNA- tyrosine residue respectively.

Explanation of Solution

It is given that the enzymes, DNA polymerase I, DNA ligase and topoisomerase I help in catalyzing the formation of phosphodiaster bonds.

The activated intermediate present in case of DNA polymerase I is deoxynucleotide triphosphate and the leaving group present in this enzyme is pyrophosphate.

The activated intermediate present in case of DNA ligase is DNA adenylate, in which AMP is attached to 5' phosphate and the leaving group present in this enzyme is adenosine monophosphate(AMP).

The activated intermediate present in case of topoisomerase I is DNA-tyrosyl intermediate, in which 5' phosphate is attached to phenolic −OH group and the leaving group present in this enzyme is the tyrosine residue.

Conclusion

The activating intermediate present in the linkage of the given enzymes are deoxynucleotide triphosphate, DNA adenylate and DNA-tyrosyl intermediate respectively. The leaving groups present in the enzymes are pyrophosphate, AMP and DNA- tyrosine residue respectively.

Answer 6

Chapter 29, Problem 1P

Interpretation Introduction

Interpretation:

The activated intermediate present in the linkage of the enzymes, DNA polymerase I, DNA ligase and topoisomerase I are to be stated. The leaving groups present in the enzymes are to be stated.

Concept introduction:

DNA stands for deoxyribonucleic acid, is a biological macromolecule. DNA contains double helical strands along with the complementary base pairs. The four complementary bases of DNA are adenine (A), thymine (T), guanine (G) and cytosine (C). In the formation of recombinant DNA, the restriction enzymes are involved to cut the particular region in the DNA molecule.

Expert Solution & Answer

Answer to Problem 1P

The activating intermediate present in the linkage of the enzymes, DNA polymerase I, DNA ligase and topoisomerase I are deoxynucleotide triphosphate, DNA adenylate and DNA-tyrosyl intermediate respectively. The leaving groups present in the enzymes, DNA polymerase I, DNA ligase and topoisomerase I are pyrophosphate, AMP and DNA- tyrosine residue respectively.

Explanation of Solution

It is given that the enzymes, DNA polymerase I, DNA ligase and topoisomerase I help in catalyzing the formation of phosphodiaster bonds.

The activated intermediate present in case of DNA polymerase I is deoxynucleotide triphosphate and the leaving group present in this enzyme is pyrophosphate.

The activated intermediate present in case of DNA ligase is DNA adenylate, in which AMP is attached to 5' phosphate and the leaving group present in this enzyme is adenosine monophosphate(AMP).

The activated intermediate present in case of topoisomerase I is DNA-tyrosyl intermediate, in which 5' phosphate is attached to phenolic −OH group and the leaving group present in this enzyme is the tyrosine residue.

Conclusion

The activating intermediate present in the linkage of the given enzymes are deoxynucleotide triphosphate, DNA adenylate and DNA-tyrosyl intermediate respectively. The leaving groups present in the enzymes are pyrophosphate, AMP and DNA- tyrosine residue respectively.

Answer 7

Chapter 29, Problem 1P

Interpretation Introduction

Interpretation:

The activated intermediate present in the linkage of the enzymes, DNA polymerase I, DNA ligase and topoisomerase I are to be stated. The leaving groups present in the enzymes are to be stated.

Concept introduction:

DNA stands for deoxyribonucleic acid, is a biological macromolecule. DNA contains double helical strands along with the complementary base pairs. The four complementary bases of DNA are adenine (A), thymine (T), guanine (G) and cytosine (C). In the formation of recombinant DNA, the restriction enzymes are involved to cut the particular region in the DNA molecule.

Answer 8

Expert Solution & Answer

Answer to Problem 1P

The activating intermediate present in the linkage of the enzymes, DNA polymerase I, DNA ligase and topoisomerase I are deoxynucleotide triphosphate, DNA adenylate and DNA-tyrosyl intermediate respectively. The leaving groups present in the enzymes, DNA polymerase I, DNA ligase and topoisomerase I are pyrophosphate, AMP and DNA- tyrosine residue respectively.

Answer 9

Expert Solution & Answer

Answer 10

Expert Solution & Answer

Answer 11

Explanation of Solution

It is given that the enzymes, DNA polymerase I, DNA ligase and topoisomerase I help in catalyzing the formation of phosphodiaster bonds.

The activated intermediate present in case of DNA polymerase I is deoxynucleotide triphosphate and the leaving group present in this enzyme is pyrophosphate.

The activated intermediate present in case of DNA ligase is DNA adenylate, in which AMP is attached to 5' phosphate and the leaving group present in this enzyme is adenosine monophosphate(AMP).

The activated intermediate present in case of topoisomerase I is DNA-tyrosyl intermediate, in which 5' phosphate is attached to phenolic −OH group and the leaving group present in this enzyme is the tyrosine residue.

Answer 12

Conclusion

The activating intermediate present in the linkage of the given enzymes are deoxynucleotide triphosphate, DNA adenylate and DNA-tyrosyl intermediate respectively. The leaving groups present in the enzymes are pyrophosphate, AMP and DNA- tyrosine residue respectively.

Answer 13

Ch. 29 - Prob. 1P Ch. 29 - Prob. 2P Ch. 29 - Prob. 3P Ch. 29 - Prob. 4P Ch. 29 - Prob. 5P Ch. 29 - Prob. 6P Ch. 29 - Prob. 7P Ch. 29 - Prob. 8P Ch. 29 - Prob. 9P Ch. 29 - Prob. 10P

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