Biology: Life on Earth with Physiology (11th Edition)
Biology: Life on Earth with Physiology (11th Edition)
11th Edition
ISBN: 9780133923001
Author: Gerald Audesirk, Teresa Audesirk, Bruce E. Byers
Publisher: PEARSON
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Chapter 25, Problem 5RQ

List the adaptations that distinguish reptiles from amphibians and help reptiles adapt to life in dry terrestrial environments.

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True or False? a.____If a restriction enzyme recognizes the restriction site, 5’AACGTT3’, and the enzyme cuts between the second A and the C, this will produce a “sticky end,” which is useful for cloning into a plasmid vector.   b.____The DNA molecules will travel through the agar toward the negative end of the gel rig (black wires) during electrophoresis.   c.____CRISPR was one of the first methods discovered in the 1970s for reliably amplifying gene products so that they can be sequenced and studied.
a) Describe the discovery of polymerase chain reaction (PCR), the components of the reaction and the generalized steps to the reaction  b) Describe how restriction enzymes like EcoR1 are used to create recombinant plasmids and what the process is for using these plasmids to replicate a piece of target DNA. Include information about how to create sticky ends, the makeup of the bacterial plasmid and how to tell if the gene was successfully inserted in the plasmid and if the plasmid has been transformed by the bacteria. You may use a drawing to enhance your description
a) Long interspersed elements (LINEs) are a type of b) Sequences of DNA assembled by identifying overlaps among smaller DNA segments are known as c) Humans are diploid organisms, containing two sets of chromosomes. How many sets of chromosomes does wheat,Triticum aestivum, have? d) Your research group has recently determined the complete genomic sequence of the organism Examus biologisium. You instruct your group to begin sequence annotation, which involves
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