Human Anatomy
9th Edition
ISBN: 9780135168059
Author: Marieb, Elaine Nicpon, Brady, Patricia, Mallatt, Jon
Publisher: Pearson Education, Inc.,
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Chapter 25, Problem 2CRCAQ
Based on what you know about the sources of semen, explain the effect of a vasectomy on the volume of the ejaculate.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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purple after tetra-methyl-p-phenylenediamine dihydrochloride (TMPD) is added. In the
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oxygen is terminal electron acceptor
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Chapter 25 Solutions
Human Anatomy
Ch. 25 - Where are interstitial cells located? What is...Ch. 25 - Distinguish the tunica albuginea from the tunica...Ch. 25 - What is meant by the phrase "sperm mature in the...Ch. 25 - Where is the ejaculatory duct located?Ch. 25 - Which pair of accessory glands produces the...Ch. 25 - Which erectile tissue does the urethra pass...Ch. 25 - How many spermatids are formed from each primary...Ch. 25 - What roles do sustentocytes play in sperm...Ch. 25 - What are the components of the head of the sperm?...Ch. 25 - What type of tissue lines the uterine tubes? How...
Ch. 25 - Name the peritoneal supports of the female...Ch. 25 - Describe the location of each of the following...Ch. 25 - What are the male homologues of the following...Ch. 25 - Which hormone stimulates follicle development in...Ch. 25 - During which period of a female’s life do stem...Ch. 25 - What is the corpus luteum, and what role does it...Ch. 25 - When does the glandular portion of the breast...Ch. 25 - What prevents more than one sperm from fertilizing...Ch. 25 - What part of the placenta is formed from maternal...Ch. 25 - In addition to nourishing the developing fetus,...Ch. 25 - What type of screening is most common for each...Ch. 25 - What do the embryonic mesonephric ducts form in a...Ch. 25 - What are secondary sex characteristics in males...Ch. 25 - Which of the following statements about the female...Ch. 25 - For each of the following hollow organs; choose...Ch. 25 - Which of the following structures produces the...Ch. 25 - The uterine cycle can be divided into three...Ch. 25 - Prob. 5RQCh. 25 - The hormone-secreting layer of the placental...Ch. 25 - The function of the stereocilia in the duct of the...Ch. 25 - The broad ligament of the uterus in the female...Ch. 25 - The embryonic paramesonephric duct gives rise to...Ch. 25 - Which of the following statements about the...Ch. 25 - If the uterine tube is a trumpet ("salpinx"), what...Ch. 25 - The myometrium is the muscular layer of the...Ch. 25 - What occurs during the proliferative phase of the...Ch. 25 - Which cells in the ovaries secrete hormones? (a)...Ch. 25 - Prob. 15RQCh. 25 - The hormones that stimulate the ovarian cycle are...Ch. 25 - Sustentocytes (a) nourish spermatocytes and....Ch. 25 - Match the embryonic structures listed in column B...Ch. 25 - Why would a radical mastectomy affect ability to...Ch. 25 - Based on what you know about the sources of semen,...Ch. 25 - A 68-year-old man who has trouble urinating is...Ch. 25 - Gina gave birth to six children. During an...Ch. 25 - Why do doctors often recommend that men not wear...Ch. 25 - Explain how a woman with an ovary removed from one...Ch. 25 - In severe cases of endometriosis, endometrial...Ch. 25 - Name three sets of arteries that must be...
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- You will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. You have already cultured it and gone through the plate isolation procedure. Before you ship your samples off for sequencing, you want to do one final check of the A260 ratios. You get back the following ratios: A260/280 ratio is 1.89; A260/230 is 2.01. These ratios are close enough to the accepted "pure" values so they could be considered "pure" and mostly (if not completely) free of contaminants from the PCR process. True Falsearrow_forwardYou will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. After receiving your sequence back from the sequencing lab, you feel that you have, in fact, discovered and isolated a new species. You ask a fellow labmate about how you should proceed, and he tells you the following is the proper way to introduce a new species for recognition: Cultures have to be sent to international culture collections. Then a paper must be published describing the new organism and providing a genus and species name. You recall learning about this in your Microbiology course in college. Is this information from your colleague true or false? True Falsearrow_forwardis often a good indication of phylogenetic relatedness in phenotypes. Life-cycle patterns Cleavage patterns O Gene expression O Morphological similarityarrow_forward
- Which of the following is a weakness of using 16S rRNA for phylogenetic analyses? It can only go down to the family and genus levels It takes months to complete O Both of the above O None of the abovearrow_forwardAn unrooted tree containing ten unrelated species can become rooted by adding a descendant group related to two of the species. an unrelated outgroup. O a distantly related outgroup. O a descendant related to only one of the species.arrow_forwardWhat is the most appropriate purpose of building a phylogenetic tree? They look awesome You can use a tree to compare morphological characteristics of organisms It can be used to establish and analyze evolutionary relationships between species All of the abovearrow_forward
- Which of the following sequencing techniques can identify down to the strain level? O Multilocus sequence typing Genomic fingerprinting Whole genome sequencing OSNP analysis All of the abovearrow_forwardWhat is the "gold standard" that is currently applied to species designations in microbiology? 97% between species: 50% among whole genome 90% between species: 75% among whole genome 99% between species; 97% among whole genome 97% between species: 70% among whole genome Onone of the abovearrow_forwardYou will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. You have decided to send your sample off for sequencing. You need to determine which type of sequencing to use for the preliminary identification of your species. You decide that, for now, you only need to be able to identify the family and genus levels. Which type of sequencing do you think is the most appropriate? O Genomic Fingerprinting O Whole Genome Sequencing O 16S rDNA Sequencing O DNA-DNA hybridization Nextarrow_forward
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