Converting dc to ac. An individual cell such as an egg cell (an ovum, produced in the ovaries) is commonly organized spatially, as manifested in part by asymmetries in the cell membrane These asymmetries include non-uniform distributions of ion transport mechanisms, which result in a net
These cellular currents move in loops through extracellular fluid Ohm s law requires that there be a voltage difference between any two points in this current-carrying fluid surrounding cells Although the currents may be significant, the extracellular voltage differences are tiny—on the order of nanovolts If we can map the voltage differences in the fluid outside a cell, we can calculate the current density by using Ohm s law, assuming that the resistivity of the fluid is known We cannot measure these voltage differences by spacing two electrodes 10 or 20 μm apart because the dc impedance (the resistance) of such electrodes is high and the inherent noise in signals detected at the electrodes far exceeds the cellular voltages.
One successful method of measurement uses an electrode with a ball-shaped end made of platinum that is moved sinusoidally between two points in the fluid outside a cell The electric potential that the electrode measures, with respect to a distant reference electrode, also vanes sinusoidally The dc potential difference between the two extremes (the two points in the fluid) is then converted to a sine-wave ac potential difference The platinum electrode behaves as a capacitor in series with the resistance of the extracellular fluid This resistance, called the access resistance (RA), has a value of about ρ/10a, where ρ is the resistivity of the fluid (usually expressed in ω cm) and a is the radius of the ball electrode The platinum ball typically has a diameter of 20 pm and a capacitance of 10 μm; the resistivity of many biological fluids is 100 ω cm.
43. If the frequency at which the electrode is oscillated is increased to a very large value, what happens to the electrode s impedance? The impedance
- A. approaches infinity.
- B. approaches zero.
- C. approaches a constant but nonzero value.
- D. does not change.
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