All the purple non-sulfur bacteria belong to α-proteobacteria, except Rhodocyclus. Rhodospirillum rubrum is a purple non-sulfur bacteria. It is a gram-negative, facultative anaerobe and is spiral shaped. R. rubrum is found in most of the natural aquatic environment such as lakes, ponds, streams, and also in sewage and mud.
Explanation of Solution
The advantages of
The presence of intra-cytoplasmic membranes offsets the energetic burden of the organism. These sites are responsible for the generation of energy. The photosynthetic apparatus is present in the intra-cytoplasmic membrane. It is the place where
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Chapter 22 Solutions
Prescott's Microbiology
- A classic way to isolate thymidylate synthase–negative mutants of bacteriais to treat a growing culture with thymidine and trimethoprim. Most ofthe cells are killed, and the survivors are greatly enriched in thymidylatesynthase–negative mutants.(a) What phenotype would allow you to identify these mutants?(b) What is the biochemical rationale for the selection? (That is, why are themutants not killed under these conditions?)(c) How would the procedure need to be modified to select mammalian cellmutants defective in thymidylate synthase?arrow_forward. Mutants of Neurospora crassa that lack carbamoyl phosphate syn- thetase I (CPS I) require arginine in the medium in order to grow, whereas mutants that lack carbamoyl-phosphate synthetase II (CPS II) require a pyrimidine, such as uracil. A priori, one would expect the active CPS II in the arginine mutants to provide sufficient carbamoyl phosphate for arginine synthesis, and the active CPS I in the pyrimidine mutants to "feed" the pyrimidine pathway. Explain these observations.arrow_forwardIn Aplysia, (a) why does a gentle touch on the siphon lead to pronounced gill withdrawal after electric shock on its tail? (b) why does a gentle touch on the syphon lead to even more pronounced gill withdrawal after pairing electric shock on its tail with siphon touch? (c) you found an Aplysia mutant that cannot make functional adenylyl cyclase in the brain. How would this affect part (a) and (b)? Briefly explain your reasoning.arrow_forward
- There are three different chitin synthase genes that control the chitin synthesis in Saccharomyces cerevisiae, namely CHS I, CHSII, and CHSIII. If a mutation occurs in each of these genes, what will happen to the S. cerevisiae?arrow_forwardCTP synthetase catalyzes the glutamine-dependent conversion of UTP to CTP. The enzyme is allosterically inhibited by the product, CTP. Mammalian cells defective in this allosteric inhibition are found to have a complex phenotype: They require thymidine in the growth medium, they have unbalanced nucleotide pools, and they have an elevated spontaneous mutation rate. Explain the likely basis for these observations.arrow_forwardFor the lac genotypes of Escherichia coli shown in the following Table 1, predict the expression of beta-galactosidase (Z) and permease (Y) is inducible or noninducible or constitutive. Explain your answer. Table 1 Genotype I-P+O+Z+Y+ (i) (ii) I+P+OCZ+Y+ (iii) ISP+O+Z+Y+ (iv) I+P+O+Z+Y-//I+P-O+Z+Y+ (v) ISP+OcZ+Y+//I-P+O+Z+Y- Condition No lactose No lactose lactose lactose No lactosearrow_forward
- Why are regulatory mutants used to increase the production of glutamic acid by Corynebacterium glutamicum?arrow_forwardFor each of the following mutant E. coli strains,plot a 30-minute time course of concentration ofβ-galactosidase, permease, and acetylase enzymesgrown under the following conditions: For the first10 minutes, no lactose is present; at 10 minutes, lactosebecomes the sole carbon source. Plot concentration onthe y-axis, time on the x-axis. (Don’t worry about theexact units for each protein on the y-axis.)a. I− P+ o+ Z+ Y+ A+ / I+ P+ o+ Z− Y+ A+b. I− P+ ocZ+ Y+ A− / I+ P+ o+ Z− Y+ A+c. IsP+ o+ Z+ Y+ A+ / I− P+ o+ Z− Y+ A+d. I− P− o+ Z+ Y+ A+ / I− P+ ocZ+ Y− A+e. I− P+ o+ Z− Y+ A+ / I− P− ocZ+ Y− A+arrow_forwardIn the: A mutated TBP protein Explain: (a) What is the process affected? (b) What is the Effect on the process? (c) Does it affect prokaryotes, eukaryotes or both?arrow_forward
- The synthesis of arginine by Nuerospora was determined by examining a number of mutant strains that were unable to synthesize the compound. Use the table of bacterial growth below to 1) determine the correct sequence of the synthesis pathway and 2) where in the synthesis pathway each mutation interrupts the synthesis. A “+" indicates growth. Nothing added to Succinate Ornithine added Strain Cirtulline Arginine Added added added growth medium Wild Mutant 1 Mutant 2 Mutant 3 Mutant 4arrow_forwardIllustrate and explain how SPO11 releases SPO11-linked oligonucleotides during the zygotene stage.arrow_forwardWhat are the reagents and materials used/needed in the extraction of invertase from yeast? for a lab experiment regarding enzymes.arrow_forward
- Biology: The Dynamic Science (MindTap Course List)BiologyISBN:9781305389892Author:Peter J. Russell, Paul E. Hertz, Beverly McMillanPublisher:Cengage Learning