Biology
Biology
4th Edition
ISBN: 9781259188121
Author: Peter Stiling, Robert Brooker, Linda Graham, Eric Widmaier
Publisher: McGraw-Hill Education
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Chapter 2.1, Problem 3EQ
Summary Introduction

To describe: The interpretation of the data “98% of particles passed through the gold foil.”

Introduction: Atom is the smallest structural unit of any matter. Combination of a great number of atoms results in the origination of a substance. The living organisms are also originated from the combination of atoms. A group of atoms joined together and forms the molecule.

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(b) Table Q1 below provides optical measurements of samples with known concentrations of analyte (ten samples were measured and the average and standard deviation are presented). They are also represented graphically in Figure Q1, below the table. Analyte concentration (nM) 0.1 0.2 0.5 1 2 5 10 15 fluorescence intensity 300000 250000 200000 150000 100000 50000 0 0 Table Q1 Fluorescence intensity (average) 9300 9200 10800 19600 37200 90000 178000 224000 5 [Analyte]/nM 10 Standard deviation 2000 1200 1000 2000 10000 30000 25000 40000 15 Figure Q1. Signal intensity for different analyte concentrations. Error bars are standard deviation. (ii) Calculate the limit of detection (LOD) for this sensor. (iii) A patient sample measured using this biosensor in the diagnostic laboratory generates a signal of 142800. The coefficient of variation of the device is 5%. Explain whether the diagnostic lab would have the confidence to report that the sample has a concentration in analyte of 8 nM or…
explain the results of this experiment Table 1 Absorption Spectrum of Cobalt Chloride Using a Spectrophotometer       Wavelength           Absorbance 400 410 420 430 440 450 460 370 480 490 500 510 520 530 540 550 560 570 580 590 600 610 620 630 640 650 660 670 680 690 700 0.036 0.044 0.063 0.095 0.147 0.215 0.284 0.329 0.362 0.402 0.450 0.479 0.464 0.398 0.314 0.216 0.142 0.087 0.054 0.041 0.036 0.036 0.034 0.028 0.030 0.023 0.023 0.023 0.014 0.014 0.011         Test Tube Number Cobalt Chloride Concentration    (mol/mL) Absorbance at 510 nm       1 0.000 0.000 2 0.009 0.042 3 0.018 0.92 4 0.027 0.139 5 6 0.036 0.045 0.190 0.236   7   (Unknown)   0.151
* You can keep extra decimal places through most of this problem, but round your final estimate to one decimal place* You wish to quantify your favourite compound in an experimental solution using spectrophotometry. First, you use standard solutions of your favourite compound to collect the following standard curve data. Calculate the slope of the line of best fit. Standard curve data Conc (mM) 0.300 0.600 0.900 1.200 1.500 Abs 0.150 0.300 0.450 0.600 0.750 Next, you prepare an experimental sample by mixing 0.50 mL of the compound solution with 9.50 mL of distilled water, and you read an absorbance of 0.625 for this sample. Calculate the concentration (mM) of the original solution of compound. Round this answer to one decimal place.
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